A Comparison of Two Methods for the Preparation Cefquinome-Loaded Gelatin Microspheres for Lung Targeting

Qu, Shaoqi; Dai, Cunchun; Yang, Fenfang; Huang, Tingting; Xu, Tianli; Zhao, Li; Li, Yuwen; Hao, Zhihui

doi:10.1007/s11095-018-2342-4

Cited by 15 publications

(10 citation statements)

References 28 publications

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“…For light microscopy examination of histological sections, the right kidneys from 5 rats in each group were processed and fixed in 10% neutral buffered formalin. The formalin fixed tissue was embedded in paraffin, sliced into 4-m tissue sections and stained with hematoxylin-eosin (H&E) (48,49). Five coded sections from each group were evaluated blindly by a pathologist who was not revealed of the treatment scheme implemented as described previously (50).…”

Section: Methodsmentioning

confidence: 99%

Rutin Attenuates Vancomycin-Induced Nephrotoxicity by Ameliorating Oxidative Stress, Apoptosis, and Inflammation in Rats

Dai

Lang

et al. 2019

Antimicrob Agents Chemother

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Nephrotoxicity is the major limiting factor for the clinical use of vancomycin (VCM) for treatment of serious infections caused by multiresistant Gram-positive bacteria. This study investigated the renal protective activity of rutin in a rat model of VCM-induced kidney injury in male Wistar rats. VCM administered intraperitoneally at 200 mg/kg twice daily for 7 successive days resulted in significant elevation of blood urea nitrogen and creatinine, as well as urinary N-acetyl-β-D-glucosaminidase. Coadministration of VCM with oral rutin at 150 mg/kg significantly reduced these markers of kidney damage. Rutin also significantly attenuated VCM-induced oxidative stress, inflammatory cell infiltration, apoptosis, and decreased interleukin-1β and tumor necrosis factor alpha levels (all P < 0.05 or 0.01) in kidneys. Renal recovery from VCM injury was achieved by rutin through increases in Nrf2 and HO-1 and a decrease in NF-κB expression. Our results demonstrated a protective effect of rutin on VCM-induced kidney injury through suppression of oxidative stress, apoptosis, and downregulation of the inflammatory response. This study highlights a role for oral rutin as an effective intervention to ameliorate nephrotoxicity in patients undergoing VCM therapy.

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Section: Methodsmentioning

confidence: 99%

Rutin Attenuates Vancomycin-Induced Nephrotoxicity by Ameliorating Oxidative Stress, Apoptosis, and Inflammation in Rats

Dai

Lang

et al. 2019

Antimicrob Agents Chemother

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“…This passive lung targeting strategy is currently approved in nuclear medicine for determining lung perfusion in humans with 99m Tc-labeled macroaggregated albumin (MAA) (Pulmolite, Pharmalucence, MA, USA; DraxImage, Draxis Health, Montreal, Canada), where only 0.5–0.7% of healthy lung capillaries are embolized after IV injection of a standard dose of MAA ( i.e. , 200,000–700,000 MAA particles). − Passive lung targeting was also recently investigated in preclinical work for the treatment of lung tumors (with cisplatin), tuberculosis (with rifampicin), and pulmonary infections (with erythromycin, azithromycin, ofloxacin, and cefquinome , ). However, previous works that evaluated particles for IV treatment of pulmonary infection displayed a particle size distribution in the range of 3–50 μm.…”

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confidence: 99%

Monosized Polymeric Microspheres Designed for Passive Lung Targeting: Biodistribution and Pharmacokinetics after Intravenous Administration

et al. 2020

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Local as well as systemic therapy is often used to treat bacterial lung infections. Delivery of antibiotics to the vascular side of infected lung tissue using lung-targeting microspheres (MS) is a good alternative to conventional administration routes, allowing for localized high levels of antibiotics. This delivery route can also complement inhaled antibiotic therapy, especially in the case of compromised lung function. We prepared and characterized monodisperse poly(lactic-co-glycolic acid) (PLGA) MS loaded with levofloxacin using a flow-focusing glass microfluidic chip. In vitro characterization showed that the encapsulated LVX displayed a biphasic controlled release during 5 days and preserved its antibacterial activity. The MS degradation was investigated in vitro by cross-sectioning the MS using a focused ion beam scanning electron microscope and in vivo by histological examination of lung tissue from mice intravenously administered with the MS. The MS showed changes in the surface morphology and internal matrix, whereas the degradation in vivo was 3 times faster than that in vitro. No effect on the viability of endothelial and lung epithelial cells or hemolytic activity was observed. To evaluate the pharmacokinetics and biodistribution of the MS, complete quantitative imaging of the 111indium-labeled PLGA MS was performed in vivo with single-photon emission computed tomography imaging over 10 days. The PLGA MS distributed homogeneously in the lung capillaries. Overall, intravenous administration of 12 μm PLGA MS is suitable for passive lung targeting and pulmonary therapy.

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“…The samples were dewaxed in xylene and dehydrated in serial dilutions of alcohol and then processed in paraffin. These samples were then cut at 4 μm thicknesses using a microtome and counter‐stained with hematoxylin and eosin (H and E) for histopathological analysis by light microscopy (Qu et al, 2017, 2018). The slides were examined by a pathologist‐blinded to the treatment groups and a semiquantitative assessment of renal histological damage was performed and a semi‐quantitative score (SQS) was used as described (Roberts et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

Section: Histopathological Examinationmentioning

confidence: 99%

Chlorogenic acid prevents vancomycin‐induced nephrotoxicity without compromising vancomycin antibacterial properties

Dai

Hao

et al. 2020

Phytotherapy Research

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Vancomycin (VCM) is an effective chemotherapeutic agent commonly used against gram-positive microorganisms but has serious nephrotoxic side effects that limit its effectiveness. New therapeutics and strategies are urgently needed to combat VCM associated nephrotoxicity. In this study, we determined the protective effect of chlorogenic acid (CA) in a rat model of VCM-induced nephrotoxicity. VCM administration led to markedly elevated blood urea nitrogen and serum creatinine levels that could be prevented with CA co-administration. VCM-mediated oxidative stress was also significantly attenuated by CA as reflected by decreased malondialdehyde and nitric oxide in VCM-treated kidneys. CA administration also prevented the VCM-mediated decrease in the renal antioxidative enzyme activities of glutathione reductase, glutathione peroxidase, and catalase and led to increased levels of reduced glutathione that had been depleted by VCM. Moreover, CA administration clearly inhibited VCM-induced expression of nuclear factor-kappa B, inducible nitric oxide synthase and the downstream pro-inflammatory mediators tumor necrosis factor-α and interleukins 1β and 6. Apoptotic markers were also markedly down-regulated with CA. Overall, CA treatment mitigated VCM-induced oxidative and nitrosative stresses and countered the apoptotic and inflammatory effects of VCM. Notably, CA did not affect the antibacterial activity of VCM in vitro.

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A Comparison of Two Methods for the Preparation Cefquinome-Loaded Gelatin Microspheres for Lung Targeting

Cited by 15 publications

References 28 publications

Rutin Attenuates Vancomycin-Induced Nephrotoxicity by Ameliorating Oxidative Stress, Apoptosis, and Inflammation in Rats

Rutin Attenuates Vancomycin-Induced Nephrotoxicity by Ameliorating Oxidative Stress, Apoptosis, and Inflammation in Rats

Monosized Polymeric Microspheres Designed for Passive Lung Targeting: Biodistribution and Pharmacokinetics after Intravenous Administration

Chlorogenic acid prevents vancomycin‐induced nephrotoxicity without compromising vancomycin antibacterial properties

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