A Comparison of Two Principles for Evaluating Corneal Endothelial Viability

Stocker, Frederick W.; King, Elizabeth H.; Lucas, David O.; Georgiade, Nicholas G.

doi:10.1001/archopht.1966.03850010838011

Cited by 11 publications

(4 citation statements)

References 5 publications

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“…Corneal endothelial viability can be evaluated in several ways: staining with trypan blue, 31 nitro‐blue tetrazolium, 32 or acridine orange/ethidium bromide; 33 bicarbonate flux measurement; 34 transmission electron microscopy; 35 SEM; 36 specular microscopy (SM); 3 , 37 –39 and nuclear magnetic resonance 40 . In the present study vital staining and SEM were utilized to evaluate the corneal endothelium.…”

Section: Discussionmentioning

confidence: 99%

“…Human corneas stored in Optisol-GS have been shown to have a high percentage of viable endothelial cells after 21 days of storage at 4 8C, but have 95±100% loss of viability after 67 days. 30 Corneal endothelial viability can be evaluated in several ways: staining with trypan blue, 31 36 specular microscopy (SM); 3,37±39 and nuclear magnetic resonance. 40 In the present study vital staining and SEM were utilized to evaluate the corneal endothelium.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Optisol‐GS and neomycin‐polymyxin B‐gramicidin ophthalmic solution for corneal storage in the dog

Andrew

Samuelson

Lewis

et al. 1999

Veterinary Ophthalmology

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The objective of the research was to compare the efficacy of Optisol-GS (OGS, Bausch & Lomb Surgical, Irvine, CA, USA) with triple antibiotic ophthalmic solution (neomycin-polymyxin B-gramicidin, NPG; Bausch & Lomb, Tampa, FL, USA) in preserving the viability of corneal endothelial cells. The study subjects were thirty young to middle-aged dogs with no gross corneal pathology that had been euthanized by pentobarbital overdose for reasons unrelated to this project. Corneal tissues were harvested, analyzed, and randomly assigned to treatment groups: one of two media (OGS or NPG), and one of five storage times (1, 7, 14, 21, or 35 days). Six corneas were stored in each medium for each time period. Corneal endothelial cell viability was evaluated pre- and poststorage by vital staining (trypan blue and alizarin red S), and endothelial cell morphology was evaluated with scanning electron microscopy. Storage in NPG caused significant loss (100%) of endothelial cells after all storage times. OGS storage maintained a high level of endothelial cell viability up to 21 days (98.9% +/- 1.3% viability). A significant decrease in percentage viability was also found for OGS-stored corneas between 21 and 35 days, when endothelial cell viability decreased to 61.4% +/- 45.9%. The conclusions are that NPG storage at -20 degrees C is a very poor choice of media for corneal tissue banking if graft clarity is the goal. Storage in Optisol-GS at 4 degrees C for up to 21 days resulted in significantly higher percentages of viable endothelial cells. Optisol-GS storage should facilitate corneal preservation for canine keratoplasty patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of Optisol‐GS and neomycin‐polymyxin B‐gramicidin ophthalmic solution for corneal storage in the dog

Andrew

Samuelson

Lewis

et al. 1999

Veterinary Ophthalmology

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“…Hence, a lot of cases showed unreliability because of the indirect results [8,9]. The use of trypan blue in the examination of donor corneas, after having been first advocated by Stocker et al [7] in 1966, did not become popular. The reason was the danger of contamination or damaging of the endothelial sheet during the different steps of the procedure [9,11,12].…”

Section: Discussionmentioning

confidence: 99%

“…Although a lot of methods have been proposed to determine the suitability of the tissue for transplantation, only few have been accepted by eye banks. Such methods are the slit-lamp examination, the specular microscopy, introduced by Maurice in 1968 [6] and trypan blue staining, first suggested by Stocker et al [7] in 1966. The histochemical method introduced by Kaufman and Robbins in 1965 [7] and the test of temperature reversal, first suggested by Sherrard [4] in 1973, are not used nowadays.…”

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confidence: 99%

The Evaluation of Corneal Graft Tissue by the Use of Trypan Blue

Georgiadis

Kardasopoulos²,

Bufidis³

1998

Ophthalmologica

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Background: The quality of the donor cornea, which basically depends on the condition of the endothelial sheet, is an important factor for the successful outcome of penetrating keratoplasty. Patients and Methods: In this retrospective study, we present our experience in using trypan blue staining for the examination and evaluation of 498 consecutive corneal grafts from 251 donors, concerning their suitability for penetrating keratoplasty, during the decade 1986–1995. Results: Forty-two grafts out of 498 (8.5%) were considered not suitable for surgery because of the great number of destroyed cells (cell density less than 1,500 cells/mm²) and the abnormal cell size and cell shape. Nine out of the 456 (1.97%) operated eyes exhibited primary donor failure and needed re-operation, probably because of boundary cell density of the graft (ranged in those cases from 1,600 to 1,800 cells/mm²). No infections of grafts were observed. Conclusion: Tissue evaluation of corneal grafts by the use of trypan blue staining is a simple, cost-effective, safe and accurate method that does not influence the vitality of the endothelial layer of the grafts.

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