A Comparison of Two Ultrasonic Methods for Detaching Biofilms from Natural Substrata

Mermillod‐Blondin, Florian; Fauvet, Guillaume; Chalamet, A.; Châtelliers, Michel Creuzé des

doi:10.1002/1522-2632(200106)86:3<349::aid-iroh349>3.3.co;2-2

Cited by 5 publications

(8 citation statements)

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“…, ; Mermillod‐Blondin et al . ) reported that the successful utilization of ultrasonic apparatus operating at a low frequency (<100 kHz) to dislodge biofilms. As seen from our results, in addition to intensity of acoustic energy and frequency, effectiveness of ultrasound treatment is dependent on the treatment time; extended treatments are generally more effective (Scherba et al .…”

Section: Resultsmentioning

confidence: 99%

Combined effect of benzalkonium chloride and ultrasound againstListeria monocytogenesbiofilm on plastic surface

Torlak

Sert

2013

Lett Appl Microbiol

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Significance and Impact of the Study: Listeria monocytogenes is capable of adhering and forming biofilm on inert surfaces such as plastics. Biofilms are more resistant to disinfectants compared to planktonic cells, and this makes their elimination from food processing facilities a big challenge. Moreover, the emergence of resistant to disinfectants bacteria clearly shows that new biofilm control strategies are required. Among new strategies, the ultrasound treatment attracts attention as a nonchemical and environmentally friendly technology. This study demonstrated that the combination of ultrasound and benzalkonium chloride is a feasible strategy for removal of L. monocytogenes biofilms from plastic food-contact surfaces. AbstractThis study was performed to evaluate the effectiveness of benzalkonium chloride combined with ultrasound in eliminating Listeria monocytogenes biofilm from polystyrene surface. The test strain of L. monocytogenes, previously classified as strong biofilm producer, was grown to form biofilm in tryptic soy broth at 20°C for 6 days in polystyrene specimen containers. The biofilms formed on surface of containers were treated with ultrasound alone, benzalkonium chloride (100 and 400 mg l À1 ) alone and their combination for three different exposure times (1, 5 and 15 min) at room temperature. Sonication was performed using an ultrasound bath at a constant ultrasound frequency of 35 kHz. After treatments, levels of biofilm biomass and viable cells in biofilm were determined using crystal violet staining and XTT assays, respectively. The combined treatment of ultrasound and benzalkonium chloride resulted in significant (P < 0Á05) more decrease in the level of viable cells in the L. monocytogenes biofilm compared to individual treatments of benzalkonium chloride. Our results suggest that the combination of benzalkonium chloride with ultrasound is useful approach for the elimination of L. monocytogenes biofilms from plastic surfaces.

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Section: Resultsmentioning

confidence: 99%

Combined effect of benzalkonium chloride and ultrasound againstListeria monocytogenesbiofilm on plastic surface

Torlak

Sert

2013

Lett Appl Microbiol

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“…Some evidence for this conjecture is provided by studies addressing the fate of test compounds. For these reasons, there is a strong need to develop further the artificial sediment by adding other sources of organic matter and/or a bacterial inoculum extracted from natural sediment (e.g., [41]) to obtain a closer resemblance with natural microbial communities (with regard to activity and composition). In experiments with artificial sediment, we have found that less than 1% of added 14 C-lindane was recovered as 14 CO 2 in CO 2 traps after 30 d (W. Goedkoop and A-L. Haglund, unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Microbial characterization of artificial sediment and comparisons with natural sediments—implication for toxicity testing

Goedkoop

Widenfalk

Haglund

et al. 2005

Enviro Toxic and Chemistry

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The development and activity of microbiota in artificial sediment may have strong implications for the fate of test compounds and the outcome of toxicity tests. In this study, we compare a number of microbial variables in the artificial sediment commonly used in toxicity testing with that of natural sediments. Bacterial abundance of artificial sediment ranged 5.7 to 7.1 x 10(8) cells/g wet weight, which is about two orders of magnitude lower than values commonly reported for natural sediments. Similarly, alternative estimates of microbial biomass (sum of phospholipid fatty acid, ergosterol, adenosine triphosphate) were several times lower for artificial sediment compared with natural sediment. Bacterial activity (3H-thymidine incorporation) ranged 4.0 to 7.4 pmol g(-1) h(-1) (or 0.062-0.113 microg C g(-1) h(-1)) in artificial sediments, which is low compared with values commonly reported for freshwater sediments. Community respiration in artificial sediment was 34 to 93 microg CO2 g(-1) d(-1). Bacterial community composition assessed by terminal restriction fragment length polymorphism of polymerase chain reaction amplified 16S rRNA genes demonstrated that natural bacterial communities appear to be more diverse than their counterparts in artificial sediment. The average similarity of the microbial communities obtained by this method was less than 40%, and different operational taxonomic units appeared to dominate the artificial and natural sediment, respectively. These results and supporting data from previous studies in natural sediments suggest that the artificial sediment has a poorly developed microbial component that differs substantially from that in natural sediments.

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“…A Monte Carlo permutation test for forward selection indicated that ultrasonication significantly associated with the community composition (P < 0.05), but agitation did not (P > 0.05). The CCA plot indicated that ultrasonication was positively correlated with the abundances of Ohtaekwangia (1, the number in Table 2), Ferruginibacter (3), Flavobacterium (14), Novosphingobium (16), Stigmatella (19), Nannocystis (22), Anaeromyxobacter (24) and Phenylobacterium (26). Agitation seemed to have a positive effect on the abundances of Rhodobacter (2), Hyphomicrobium (5), Kofleria (8), Haliscomenobacter (10), Dongia (11) and Niastella (18).…”

Section: Effects Of Ultrasonication On Bacterial Communitymentioning

confidence: 99%

“…[10,22,23] Ultrasonication is broadly used to recover microbes and biofilms from various substrata, including activated carbon. [10,13,24] For instance, Magic-Knezev and van der Kooij [10] reported that ultrasonic powers were positively correlated with the recovered microbial cells from GAC, although they were damaged through the process. The main objective of this study was to determine effects of ultrasonication with or without agitation on bacterial DNA extraction from BAC with regard to the quantity and composition of the recovered DNA.…”

Section: Introductionmentioning

confidence: 99%

Effects of ultrasonic pretreatment on quantity and composition of bacterial DNA recovered from granular activated carbon used for drinking water treatment

Kim

Cho

2014

Journal of Environmental Science and Health, Part A

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Effects of ultrasonic pretreatment on bacterial DNA recovery from granular activated carbon (GAC) were investigated. GAC (Calgon F400), biologically activated, was sampled from an actual drinking water plant. Different ultrasonic energy densities (0-400 J·cm(-3)) were applied with agitation (250 rpm for 30 min), and recovered bacterial DNA was quantified using quantitative PCR. Energy density was linearly correlated with the concentration of carbon fines produced from GAC during ultrasonication. Ultrasonication alone had no effect on DNA recovery at ≤60 J·cm(-3), but a strongly adverse effect at >67 J·cm(-3) due to the produced carbon fines. Agitation along with ultrasonication strongly enhanced the bacterial DNA recovery when ≤40 J·cm(-3) was applied, although it did not affect the production of carbon fines. Ribosomal tag pyrosequencing was used to compare recovered bacterial communities (0, 20 and 30 J·cm(-3) with or without agitation). Ultrasonication allowed for obtaining a more diverse and richer bacterial community from GAC, compared with the control. Agitation did not show a positive effect on community organization (richness and diversity). Consistently, canonical correspondence analysis indicated that the energy density was associated with the relative abundances of particular bacterial members (P < 0.05), while agitation did not. Correspondence analysis revealed that the recovered bacterial communities were grouped according to the applied energy densities. In conclusion, ultrasonication and agitation influence the recovered DNA in quality and quantity, respectively, and carbon fines as a by-product by ultrasonication interfere with the DNA recovery.

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A Comparison of Two Ultrasonic Methods for Detaching Biofilms from Natural Substrata

Cited by 5 publications

References 0 publications

Combined effect of benzalkonium chloride and ultrasound againstListeria monocytogenesbiofilm on plastic surface

Combined effect of benzalkonium chloride and ultrasound againstListeria monocytogenesbiofilm on plastic surface

Microbial characterization of artificial sediment and comparisons with natural sediments—implication for toxicity testing

Effects of ultrasonic pretreatment on quantity and composition of bacterial DNA recovered from granular activated carbon used for drinking water treatment

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