A field experiment was conducted to study the effect of different organic manures and their levels on nutrient status and soil microbial activity in rose at Hisar during 2020-21. The treatments comprised of 3 different types of organic manures viz., farm yard manures, vermicompost and poultry manure and 4 levels control, 4 kg/m2, 5 kg/m2 and 6 kg/m2. The experiment was carried out in the split plot design with three replications. Observation on organic carbon, dehydrogenase activity and alkaline phosphatase activity, N, P and K content in rose leaves and available N, P and K content in soil at the time of harvest were recorded. Organic carbon in soil was maximum (0.93%) with the application of vermicompost while dehydrogenase and alkaline phosphatase activity were highest with the application of farmyard manure and poultry manure, respectively. Nitrogen (2.17%) and phosphorus (0.27%) content were maximum in rose leaves at harvest stage with the application of poultry manure while Potassium (1.41%) content was maximum vermicompost. Same trend was observed in the nutrient status of soil with the application of different organic manures. It is concluded that the application of poultry manures had pronounced effect on microbial activity and nutrient status in rose.