A Compartmentalized Nanoreactor Formed by Interfacial Hydrogelation for Cascade Enzyme Catalytic Therapy

Abstract

Some cellular enzymatic pathways are located within a single organelle, while most others involve enzymes that are located within multiple compartmentalized cellular organelles to realize the efficient multi‐step enzymatic process. Herein, bioinspired by enzyme‐mediated biosynthesis and biochemical defense, a compartmented nanoreactor (Burr‐NCs@GlSOD) was constructed through a self‐confined catalysis strategy with burr defect‐engineered molybdenum disulfide/Prussian blue analogues (MoS2/PBA) and an interfacial diffusion‐controlled hydrogel network. The specific catalytic mechanism of the laccase‐like superactivity induced hydrogelation and cascade enzyme catalytic therapy were explored. The confined hydrogelation strategy introduces a versatile means for nanointerface functionalization and provides insight into biological construction of simulated enzymes with comparable activity and also the specificity to natural enzymes.

Spatiotemporal Controlled Formation of Synthetic Nanoreactors in Single Living Cells

“…Compartmentalization is involved in diverse biological processes in eukaryotes . As a key feature of life, compartmentalization is widely used by cells for spatiotemporal control over various biological reactions, cellular signaling, and metabolic processes. − In living cells, subcellular compartments including phospholipid membrane-bound organelles or membraneless organelles provide a highly confined microenvironment, which enables highly efficient enzymatic reactions. , …”