A Compendium of the Principal Stationary Phases Used in Hydrophilic Interaction Chromatography: Where Have We Arrived?

Guarducci, Maria A.; Fochetti, Andrea; Ciogli, Alessia; Mazzoccanti, Giulia

doi:10.3390/separations10010022

Cited by 9 publications

(5 citation statements)

References 86 publications

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“…Thus, before the crossover point, water acts as a typical normal phase modifier, weakening the polar interactions established between fragments of ITC and polar active sites of the polysaccharide-type selector. This trend is typical of an HILIC retentive mechanism [40][41][42][43][44][45][46]. Beyond the critical mobile phase composition, the increase in water in the mobile phase triggers an increase in retention typical of the reversed-phase (RP) mechanism.…”

Section: Enantioseparations With Ethanol-based Eluentsmentioning

confidence: 93%

Green HPLC Enantioseparation of Chemopreventive Chiral Isothiocyanates Homologs on an Immobilized Chiral Stationary Phase Based on Amylose tris-[(S)-α-Methylbenzylcarbamate]

Mammone,

Panusa,

Risoluti

et al. 2024

Molecules

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Sulforaphane is a chiral phytochemical with chemopreventive properties. The presence of a stereogenic sulfur atom is responsible for the chirality of the natural isothiocyanate. The key role of sulfur chirality in biological activity is underscored by studies of the efficacy of individual enantiomers as chemoprotective agents. The predominant native (R) enantiomer is active, whereas the (S) antipode is inactive or has little or no biological activity. Here we provide an enantioselective high-performance liquid chromatography (HPLC) protocol for the direct and complete resolution of sulforaphane and its chiral natural homologs with different aliphatic chain lengths between the sulfinyl sulfur and isothiocyanate group, namely iberin, alyssin, and hesperin. The chromatographic separations were carried out on the immobilized-type CHIRALPAK IH-3 chiral stationary phase with amylose tris-[(S)-methylbenzylcarbamate] as a chiral selector. The effects of different mobile phases consisting of pure alcoholic solvents and hydroalcoholic mixtures on enantiomer retention and enantioselectivity were carefully investigated. Simple and environmentally friendly enantioselective conditions for the resolution of all chiral ITCs were found. In particular, pure ethanol and highly aqueous mobile phases gave excellent enantioseparations. The retention factors of the enantiomers were recorded as the water content in the aqueous-organic modifier (methanol, ethanol, or acetonitrile) mobile phases progressively varied. U-shaped retention maps were generated, indicating a dual and competitive hydrophilic interaction liquid chromatography (HILIC) and reversed-phase liquid chromatography retention mechanism on the CHIRALPAK IH-3 chiral stationary phase. Finally, experimental chiroptical studies performed in ethanol solution showed that the (R) enantiomers were eluted before the (S) counterpart under all eluent conditions investigated.

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Section: Enantioseparations With Ethanol-based Eluentsmentioning

confidence: 93%

Green HPLC Enantioseparation of Chemopreventive Chiral Isothiocyanates Homologs on an Immobilized Chiral Stationary Phase Based on Amylose tris-[(S)-α-Methylbenzylcarbamate]

Mammone,

Panusa,

Risoluti

et al. 2024

Molecules

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“…Recently, thanks to advances in HPLC stationary phase technology, several amino phases are available on the market that overcome these drawbacks: either replacing silica gel with a polymer support or by using a carbamoylor an amide-groups (BEH, ethylene bridged hybrid) as stationary phases [33]. Stationary phases for HILIC have been reviewed by Guarducci et al [34]. In the last decade, HILIC on poly-hydroxyl stationary phases was used for analysis, separation, and determination of saccharides [35][36][37][38][39].…”

Section: Hilic-elsdmentioning

confidence: 99%

HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

Crha,

Odedina,

Pazourek

2024

Separations

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This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, and lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative to an MS detector, and the separation system’s selectivity allows the separation of anomers of monosaccharides. The study also includes a rapid method for determining isomaltulose (Palatinose), which was validated and applied to food supplement samples available in the Czech market, even those with high protein content. Additionally, isomaltulose was separated from sucrose in just 13 min.

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“…There have been many chemistries developed for HILIC [44,45]. The amide-based chemistry has shown the best separation of N-glycans.…”

Section: Lc Separation: Hilic Coupled To Fld and Ms Detectionmentioning

confidence: 99%

Updates of the current strategies of labeling for N-glycan analysis

Helali,

Delporte

2024

Journal of Chromatography B

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A Compendium of the Principal Stationary Phases Used in Hydrophilic Interaction Chromatography: Where Have We Arrived?

Cited by 9 publications

References 86 publications

Green HPLC Enantioseparation of Chemopreventive Chiral Isothiocyanates Homologs on an Immobilized Chiral Stationary Phase Based on Amylose tris-[(S)-α-Methylbenzylcarbamate]

Green HPLC Enantioseparation of Chemopreventive Chiral Isothiocyanates Homologs on an Immobilized Chiral Stationary Phase Based on Amylose tris-[(S)-α-Methylbenzylcarbamate]

HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

Updates of the current strategies of labeling for N-glycan analysis

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