A Competitive Enzyme-Linked Immunosorbent Assay-like Method for the Measurement of Urinary Hyaluronan

Maéda, Hiroshi; Fujita, Hiroshi; Sakura, Yoshiyuki; Miyazaki, Kyosuke; Goto, Makoto

doi:10.1271/bbb.63.892

Cited by 21 publications

(11 citation statements)

References 14 publications

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“…WS has been formerly inadvertently classified as a new group of hereditary mucopolysaccharidosis (44)(45)(46). Hyaluronan elevation either from urine and serum has been believed as a biomarker of normal aging and progeroid syndromes such as WS and progeria (46)(47)(48)(49) and the International Registry of Werner syndrome included hyaluronuria for diagnosing WS (http://www. pathology.…”

Section: Brief History Of Clinical Characterization Of Werner Syndromementioning

confidence: 99%

Werner syndrome: A changing pattern of clinical manifestations in Japan (1917-2008)

et al. 2013

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Section: Brief History Of Clinical Characterization Of Werner Syndromementioning

confidence: 99%

Werner syndrome: A changing pattern of clinical manifestations in Japan (1917-2008)

et al. 2013

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“…Hyaluronan was measured by using a competitive ELISA-like method, as published previously by Lokeshwar et al 24 and by Maeda et al 25 Hyaluronic acid (ICN Biochemicals, Aurora, OH) at 0.2 mg/mL was coated on microtiter plates for binding to a biotinylated hyaluronic acid-binding peptide (bHABP) (Seikagaku, Tokyo, Japan). Samples and standards were digested with protease overnight at 37°C, to remove any potential interfering proteins.…”

Section: Assay For Hyaluronanmentioning

confidence: 99%

Inflammatory Markers and Mediators in Tracheal Fluid of Premature Infants Treated With Inhaled Nitric Oxide

et al. 2007

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“…Urine HA was measured by a competitive ELISA-like method (CEL method), utilizing competitive reaction of free HA in the sample and biotin-labeled HA for their binding to HABP (hyaluronic acid binding protein) in the solid phase [19]. Briefly, HABP solution was added to the wells of microtiter plates (Nalge-Nunc), left overnight at 4°C and washed 3 times with PBS.…”

Section: Measurement Of Urine Ha Concentrationmentioning

confidence: 99%

Elevation of Serum Hyaluronan Level in Werner’s Syndrome

Tanabe¹,

Goto²

2001

Gerontology

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Background: There is a well-established association between Werner’s syndrome (WS) and hyperhyaluronic aciduria; however, to date hyaluronan (HA) in the serum has not been statistically linked with WS. Recently, the gene that causes WS has been defined as a DNA helicase on chromosome 8, and 19 different mutations in WS patients have been identified. It is not known whether the mutation type of the Werner helicase gene affects the levels of serum and urine HA in WS patients. Objective: To evaluate the association of serum HA with WS, and the relationship of the serum and urine HA levels to the mutation type. Methods: HA both in the serum and the urine was measured in 40 patients with WS and 114 normal controls. The serum and urine HA were quantified by sandwich binding protein assay and competitive ELISA-like method, respectively. The muation on WS helicase gene was analyzed by mutant-allele-specific amplification and oligomer ligation assay. Results: WS patients showed significantly higher levels of HA in the serum (mean ± SD: 115.7 ± 119.8 ng/ml, p < 0.001) and urine (1,040.8 ± 777.3 ng/mg creatinine, Cre, p < 0.001) than age-matched controls (serum HA: 15.8 ± 14.2 ng/ml, urine HA: 379.7 ± 124.2 ng/mg Cre). The serum and urine HA levels of WS patients are almost equal to those of normal controls over 80 years (serum HA: 118.5 ± 108.4 ng/ml, urine HA: 914.5 ± 712.1 ng/mg Cre). There was a significant correlation between serum and urine HA levels in WS patients (r = 0.42, p = 0.007). Analysis of the mutation on the helicase gene in 22 WS patients showed that among 44 chromosomes, 3 (6.8%) chromosomes had type 1 mutation, 22 (50.0%) had type 4 mutation, 14 (31.8%) had type 6 mutation, and the rest had other mutations. The serum and urine levels of HA did not show any significant association with the mutation type. Conclusion: The hyperhyaluronic aciduria in WS reflects the high level of serum HA. The serum and urine HA levels are useful biochemical markers for WS irrespective of the muation type of the Werner helicase gene.

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A Competitive Enzyme-Linked Immunosorbent Assay-like Method for the Measurement of Urinary Hyaluronan

Cited by 21 publications

References 14 publications

Werner syndrome: A changing pattern of clinical manifestations in Japan (1917-2008)

Werner syndrome: A changing pattern of clinical manifestations in Japan (1917-2008)

Inflammatory Markers and Mediators in Tracheal Fluid of Premature Infants Treated With Inhaled Nitric Oxide

Elevation of Serum Hyaluronan Level in Werner’s Syndrome

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