A Competitive Inhibitor That Reduces Recruitment of Androgen Receptor to Androgen-responsive Genes

Cherian, Milu T.; Wilson, Elizabeth M.; Shapiro, David J.

doi:10.1074/jbc.m112.344671

Cited by 21 publications

(19 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Luciferase assay were as previously described (Cherian et al, 2012). Hela cells were plated (300,000 cells/well in 1 ml) in 6-well plates in 10% CD-CS 3 days before transfection.…”

Section: Methodsmentioning

confidence: 99%

Anticipatory activation of the unfolded protein response by epidermal growth factor is required for immediate early gene expression and cell proliferation

Andruska

Zheng

et al. 2016

Molecular and Cellular Endocrinology

Self Cite

View full text Add to dashboard Cite

The onco-protein epidermal growth factor (EGF) initiates a cascade that includes activation of the ERK and AKT signaling pathways and alters gene expression. We describe a new action of EGF–EGF receptor (EGFR), rapid anticipatory activation of the endoplasmic reticulum stress sensor, the unfolded protein response (UPR). Within 2 min, EGF elicits EGFR dependent activation of phospholipase C γ (PLCγ), producing inositol triphosphate (IP3), which binds to IP3 receptor (IP3R), opening the endoplasmic reticulum IP3R Ca2+ channels, resulting in increased intracellular Ca2+. This calcium release leads to transient and moderate activation of the IRE1α and ATF6α arms of the UPR, resulting in induction of BiP chaperone. Knockdown or inhibition of EGFR, PLCγ or IP3R blocks the increase in intracellular Ca2+. While blocking the increase in intracellular Ca2+ by locking the IP3R calcium channel with 2-APB had no effect on EGF activation of the ERK or AKT signaling pathways, it abolished the rapid EGF-mediated induction and repression of gene expression. Knockdown of ATF6α or XBP1, which regulate UPR-induced chaperone production, inhibited EGF stimulated cell proliferation. Supporting biological relevance, increased levels of EGF receptor during tumor progression were correlated with increased expression of the UPR gene signature. Anticipatory activation of the UPR is a new role for EGF. Since UPR activation occurs in <2 min, it is an initial cell response when EGF binds EGFR.

show abstract

“…Luciferase assay were as previously described (Cherian et al, 2012). Hela cells were plated (300,000 cells/well in 1 ml) in 6-well plates in 10% CD-CS 3 days before transfection.…”

Section: Methodsmentioning

confidence: 99%

Anticipatory activation of the unfolded protein response by epidermal growth factor is required for immediate early gene expression and cell proliferation

Andruska

Zheng

et al. 2016

Molecular and Cellular Endocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Human cervical carcinoma HeLa and HeLa-AR3A-PSA-ARE4 cells that stably express human AR (39) were propagated in minimal essential medium containing 10% fetal bovine serum. Monkey kidney CV1 and COS1 cells were maintained in Dulbecco's modified essential medium with 10% bovine calf serum and 20 mM Hepes (pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

Proto-oncogene Activity of Melanoma Antigen-A11 (MAGE-A11) Regulates Retinoblastoma-related p107 and E2F1 Proteins

Minges

Grossman

et al. 2013

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background: Primate-specific melanoma antigen-A11 (MAGE-A11) increases steroid receptor transcriptional activity and enhances prostate cancer cell growth. Results: MAGE-A11 activates E2F1 by interacting with retinoblastoma-related protein p107. Conclusion: MAGE-A11 influences cell cycle regulatory pathways in a molecular hub for transcription. Significance: MAGE-A11 is a proto-oncogene whose increased expression impacts multiple signaling mechanisms that contribute to prostate cancer growth and progression.

show abstract

“…Chromatin immunoprecipitation (ChIP) assays were performed as described previously (Cherian et al, 2012). Briefly, human hepatocytes in six-well plates were maintained for 3 to 4 days in Williams' E medium containing Primary Hepatocyte Maintenance Supplement (Life Technologies), with daily media changes.…”

Section: Methodsmentioning

confidence: 99%

CINPA1 Is an Inhibitor of Constitutive Androstane Receptor That Does Not Activate Pregnane X Receptor

Cherian

Lin

et al. 2015

Molecular Pharmacology

View full text Add to dashboard Cite

show abstract

A Competitive Inhibitor That Reduces Recruitment of Androgen Receptor to Androgen-responsive Genes

Cited by 21 publications

References 48 publications

Anticipatory activation of the unfolded protein response by epidermal growth factor is required for immediate early gene expression and cell proliferation

Anticipatory activation of the unfolded protein response by epidermal growth factor is required for immediate early gene expression and cell proliferation

Proto-oncogene Activity of Melanoma Antigen-A11 (MAGE-A11) Regulates Retinoblastoma-related p107 and E2F1 Proteins

CINPA1 Is an Inhibitor of Constitutive Androstane Receptor That Does Not Activate Pregnane X Receptor

Contact Info

Product

Resources

About