A complement to DNA barcoding reference library for identification of fish from the Northeast Pacific

Abstract

The seas of the North Pacific Ocean are characterized by a large variety of fish fauna, including endemic species. Molecular genetic methods, often based on DNA barcoding approaches, have been recently used to determine species boundaries and identify cryptic diversity within these species. This study complements the DNA barcode library of fish from the Northeast Pacific area. A library based on 154 sequences of the mitochondrial <i>COI</i> gene from 44 species was assembled and analyzed. It was found that 39 species (89%) can be unambiguously identified by the clear thresholds forming a barcoding gap. Deviations from the standard 2% threshold value resulted in detection of the species <i>Enophrys lucasi </i>in the sample, which is not typical for the eastern part of the Bering Sea. This barcoding gap also made it possible to identify naturally occurring low values of interspecific divergence of eulittoral taxa <i>Aspidophoroides</i> and the deep-sea genus <i>Coryphaenoides</i>. Synonymy of the genus <i>Albatrossia</i> in favor of the genus <i>Coryphaenoides</i> is suggested based on both the original and previously published data.

Mating Season, Egg-Laying Season, and Internal Gametic Association in the Sympatrically Occurring Fluffy Sculpin (Oligocottus snyderi) and Rosy Sculpin (O. rubellio)

“…Gene tree topologies were constructed using MEGA version X (Stecher et al, 2020), with the maximum likelihood method with GTR+G+I model and 1,000 bootstrap replicates. For species identification, 11 CO1 sequences from ten species and 19 Cytb sequences from ten species were obtained from NCBI (Tables S1 and S2; Hastings and Burton, 2008;Buser and López 2015;Turanov and Kartavtsev, 2021;Awata et al, 2022). For both trees, Scorpaenichthys marmoratus was set as the outgroup.…”

Comparison of the Efficiency of Single-Locus Species Delimitation Methods: A Case Study of a Single Lake Fish Population in Comparison against the Barcodes from International Databases

“…The model group for studying biodiversity in continental water bodies is fish, a most important biological water resource for humanity. Barcoding of fish populations has been conducted in many regions of the planet [48][49][50][51][52]. Using the example of fish, a well-studied animal group, it is interesting to compare the efficiencies of different delimitation methods in specific river basins, or even single water bodies, with adequate knowledge of the species composition developed in the course of intensive previous morphological and genetic work.…”