A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma

Spadafora, Natasha D.; Cocetta, Giacomo; Cavaiuolo, Marina; Bulgari, Roberta; Dhorajiwala, Rakhee; Ferrante, António; Spinardi, A.; Rogers, Hilary J.; Müller, Carsten T.

doi:10.1038/s41598-019-39196-0

Cited by 27 publications

(16 citation statements)

References 45 publications

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“…Moreover, SPME is based on an equilibrium process, making quantitative analyses of VOC profile changes more challenging, and can suffer from saturation. In our work on fruit aroma 47 we have favoured the use of thermal desorption tubes for capturing VOCs directly from whole or minimally processed samples, and in peach this has also proven to be feasible 48 revealing important changes in VOC composition through storage.…”

Section: Introductionmentioning

confidence: 99%

Fruit volatilome profiling through GC × GC-ToF-MS and gene expression analyses reveal differences amongst peach cultivars in their response to cold storage

Muto

Müller

Bruno

et al. 2020

Sci Rep

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Peaches have a short shelf life and require chilling during storage and transport. Peach aroma is important for consumer preference and determined by underlying metabolic pathways and gene expression. Differences in aroma (profiles of volatile organic compounds, VOCs) have been widely reported across cultivars and in response to cold storage. However, few studies used intact peaches, or used equilibrium sampling methods subject to saturation. We analysed VOC profiles using TD-GC × GC-ToF-MS and expression of 12 key VOC pathway genes of intact fruit from six cultivars (three peaches, three nectarines) before and after storage at 1 °C for 7 days including 36 h shelf life storage at 20 °C. Two dimensional GC (GC × GC) significantly enhances discrimination of thermal desorption gas chromatography time-of-flight mass spectrometry (TD-GC-ToF-MS) and detected a total of 115 VOCs. A subset of 15 VOCs from analysis with Random Forest discriminated between cultivars. Another 16 VOCs correlated strongly with expression profiles of eleven key genes in the lipoxygenase pathway, and both expression profiles and VOCs discriminated amongst cultivars, peach versus nectarines and between treatments. The cultivar-specific response to cold storage underlines the need to understand more fully the genetic basis for VOC changes across cultivars.

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Section: Introductionmentioning

confidence: 99%

Fruit volatilome profiling through GC × GC-ToF-MS and gene expression analyses reveal differences amongst peach cultivars in their response to cold storage

Muto

Müller

Bruno

et al. 2020

Sci Rep

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“…In addition, there are two types of fruit ripening, climacteric and non-climacteric, which have distinct regulatory mechanisms in determining organoleptic properties (McMurchie et al 1972). Melon is an important species to study these differences because it presents both phenotypes, having climacteric cultivars that are generally more aromatic having esters as the most prominent volatiles, while the non- Spadafora et al 2019). However, this is the rst study that presents an integrative analysis of metabolomics and gene expression of biochemical pathways related to volatile compound synthesis of non-climacteric ('Yellow') and climacteric ('Gaúcho') melons.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analyses of Volatile Metabolites Associated With Gene Expression From Non-climacteric and Climacteric Melon.

Los

Schemberger

Reis

et al. 2021

Preprint

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Melon (Cucumis melo L.) is an important species in the cucurbits family with a large economic importance in the world. Two melon cultivars commercially important in Brazil are the cultivars ‘Yellow’ and ‘Gaúcho’. In addition to the economic importance, these two cultivars display phenotypic differences in aroma, a major trait determining fruit quality. Volatile organic compounds (VOC) impart the different aroma found in these fruit and its biosynthesis is associated with fatty acid and amino acid metabolism. Using SPME-GC–MS and RT-qPCR techniques, volatile production and expression of seven genes (CmLOX9, CmLOX18, CmBCAT1, CmArAT1, CmPDC1, CmADH1 and CmAAT1) were determined during maturation and ripening. The climacteric melon ‘Gaúcho’ had a greater number and higher concentration of volatiles than that in the non-climacteric ‘Yellow’ melon. 2-Methylallyl acetate, 4-amino-1-butanol, 2-methylbutanol and ethyl 2-methylpropanoate were found in high concentrations in ripe climacteric ‘Gaúcho’ melons and were major contributors to its strong fruity aroma, but high concentrations of these volatiles were not found in non-climacteric ‘Yellow’ melons. The lipid pathway played a strong role in determining aroma composition in non-climacteric ‘Yellow’ melons. Most volatiles decreased during maturation and ripening explaining the non-aromatic characteristic of this cultivar. In climacteric ‘Gaúcho’ melons, the amino acid pathway was the main one related to the biosynthesis of esters, which contribute to the aroma of this cultivar. Volatile products of the branched chain amino acid pathway correlated with CmADH1 and CmAAT1 expression demonstrating their role in volatile synthesis of this climacteric melon cultivar. In addition, CmPDC1 contributes to the formation of aldehydes at the beginning of this pathway.

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“…Pathway biosynthesis of volatile aromas begins from 5 DAA (Day After Anthezise) to 35 (DAA) and the Cm-AAT gene is expressed very strong at the end of fruit development (Guo et al 2017;Yano et al 2018). The expression of Cm-AAT1 and Cm-AAT2 genes was reported to decrease with storage time (Spadafora et al 2019) and this was observed in the 'Hikadi' CmAAT1 gene, while in 'Hikapel' the CmAAT1 gene expression is still increased until the 10th day during storage (Figure 3). This indicates a delay in the process of biosynthesis of ester compounds so that the aroma of the 'Hikadi' melon dominates qualitatively at the time of measurement.…”

Section: Analysis Of Cm-aat1 Gene Expression From Melon Cultivars 'Himentioning

confidence: 99%

“…The expression of the Cm-AAT1 gene in melons was higher than the three genes of its family (Cm-AAT2, Cm-AAT3, and Cm-AAT4) and it was also known that the expression of the Cm-AAT3 gene was much lower than the others (Sharkawy et al 2005). Spadafora et al (2019) have also used the qRT-PCR method to look at the expression of CmAAT1 and CmAAT2 genes in melons with several treatment interactions between pre-harvest and post-harvest.…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

2020

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Abstract. Wibowo WA, Fatkhurohman MI, Daryono BS. 2020. Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’. Biodiversitas 21: 3041-3046. Melon (Cucumis melo L.) is one of the horticulture commodities that have high economic value and its needs increase continuously. Many new melon cultivars have been assembled to produce a higher quality melon. Melon 'Hikapel' developed by the Laboratory of Genetics and Breeding, Faculty of Biology UGM has distinctive character in the form of a strong aroma. This aroma is a complex mixture of various kinds of volatile compound. One of the main determinant compounds is a volatile ester, synthesized by the alcohol acyl-transferase enzyme encoded by the Cm-AAT1 gene. Characterization of Cm-AAT1 began with isolation of melon rinds to get total RNAs. Synthesis cDNA was conducted with oligo-dT primer, followed by detection of Cm-AAT1 using specific primers. A specific band was sequenced to perform phylogenetic tree. Gene expression from 4 melon cultivars, ‘Hikapel’, ‘Hikadi’, ‘Sun Lady’, and ‘Luna’ analysis was performed using relative quantitative Real-Time PCR. The results of this study showed that Cm-AAT1 owned not only by aromatic cultivars ‘Hikapel’ and ‘Hikadi’, but also owned by non-aromatic cultivars ‘Sun Lady’ and ‘Luna’. Phylogenetic analysis shows a high similarity between Cm-AAT1 on 'Hikapel' and 'Hikadi'. Gene expression analysis on 'Hikapel' increases as the process of fruit ripening during the storage period and it is in contrast to 'Hikadi' at decrease when the fruit began to enter the decay process on day 7th. Expression of Cm-AAT1 on ‘Hikapel’ was higher than ‘Hikadi’ at the peak of fruit maturity.

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A complex interaction between pre-harvest and post-harvest factors determines fresh-cut melon quality and aroma

Cited by 27 publications

References 45 publications

Fruit volatilome profiling through GC × GC-ToF-MS and gene expression analyses reveal differences amongst peach cultivars in their response to cold storage

Fruit volatilome profiling through GC × GC-ToF-MS and gene expression analyses reveal differences amongst peach cultivars in their response to cold storage

Comparative Analyses of Volatile Metabolites Associated With Gene Expression From Non-climacteric and Climacteric Melon.

Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

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