A complex pattern of changes in polysomal mRNA populations is evident in the leaves of Arabidopsis thaliana (L.) Heynh. during photoperiodic induction of flowering

Lechner, Franz J.; Rau, W.

doi:10.1007/bf00198215

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…This light/dark difference in mRNA levels was similar in direction and magnitude to the observed changes in Arabidopsis HMGCoA reductase-1 mRNA levels (Learned, 1996). Multiple unidentified mRNAs have been shown to vary diurnally in Arabidopsis (Lechner and Rau, 1993), and other plant genes have been shown to be controlled by a circadian rhythm (Deng et al, 1990;Giuliano et aL, 1988;Kloppstech, 1985). Additional experiments will be required to determine whether UBQ3 is regulated by a circadian rhythm.…”

Section: Discussionsupporting

confidence: 54%

Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes

Sun

Callis²

1997

The Plant Journal

122

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SummaryThe highly conserved protein ubiquitin is encoded by five polyubiquitin genes in Arabidopsis thaliana ecotype Columbia that have been divided into two subtypes, the UBQ31UBQ4 subtype and the UBQ101UBQ111UBQ14 subtype. Northern analysis using gene-specific oligonucleotides as hybridization probes and enzyme activity measurements from transgenic plants expressing J~-glucuronidase (GUS) under the control of individual polyubiquitin 5' flanking regions were used to determine the developmental and environmental regulation of polyubiquitin transcription and mRNA accumulation. Polyubiquitin mRNA levels within and between subtypes were independently modulated. UBQ3 mRNA levels were threefold higher than UBQ4 mRNA levels in vegetative organs, but only two-thirds of the UBQ4 mRNA levels in flowers. UBQ3 mRNA was modulated by dark/light treatments, while mRNAs from UBQ4 and all members of the other subtype were unaffected. Similarly, within the UBQIO/ UBQ11/UBQ14 subtype, UBQ11/UBQ14 mRNAs were modulated differently in seedlings after a two-hour heatshock treatment. Among all the polyubiquitin genes, UBQIO mRNA level was the most constant in all organs and environmental conditions examined. Transgenic plants transformed with a UBQIO 5' flanking region::GUS gene contained higher levels of GUS activity than transgenic plants expressing GUS under the control of UBQ3 5' flanking regions. In conclusion, the relative abundance of different Arabidopsis polyubiquitin mRNAs, even those produced from highly similar genes within a subtype, appears to be modulated independently in response to developmental and environmental cues.

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Section: Discussionsupporting

confidence: 54%

Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes

Sun

Callis²

1997

The Plant Journal

122

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“…around or soon after the critical photoperiod of 12-14 hours (P6rilleux et al 1994). Similar observations were reported in S. alba (Cremer et al 1991) and A. thaliana (Lechner and Rau 1993).…”

Section: Four Major Conclusion Can Be Drawn From the Present Worksupporting

confidence: 90%

“…This is in line with previous similar findings in the SDP Pharbitis nil (Ono et al 1991) and the LDPs S. alba (Cremer et al 1991) and A. thaliana (Lechner and Rau 1993). All these works, the present one included, may suffer some methodological limitations: important molecules may not have been detected because their size was out of range in the commonly used 2D-PAGE conditions, e.g.…”

Section: Four Major Conclusion Can Be Drawn From the Present Worksupporting

confidence: 88%

“…Sinapis alba (Cremer et al 1991) and Arabidopsis thaliana (Lechner and Rau 1993). Sinapis alba can be induced to flower by a unique 22-h LD given at 150 pmol-m-2-s-~; Lechner and Rau (1993) reported quantitative changes in 39 polysomal RNAs when plants were transferred for several cycles in 15-h LDs or in continuous light at low fluence rate (ca.…”

Section: Discussionmentioning

confidence: 99%

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Changes in gene expression in the leaf of Lolium temulentum L. Ceres during the photoperiodic induction of flowering

Périlleux¹,

Ongena²,

Bernier³

1996

Planta

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Abstract. Unifoliated plants of Lolium temulentum L.Ceres were induced to flower by a unique 24-h long day (LD) consisting of the extension of the regular 8-h short day (SD) (400 I.tmol photons-m-2-s 1, fluorescence + incandescence) with incandescence at 10-15 gmolphotons. m-2.s-~. The polyadenylated-RNA complement of leaf blade tissues was analysed at 4-h intervals during the photoperiod extension in LD vs. SD, by using two-dimensional polyacrylamide gel electrophoresis to resolve in-vitro-translated products. Of the 991 spots that were analysed, none appeared or disappeared during the inductive cycle, i.e. no qualitative effect of floral induction was detected, at any time. Sixty-eight spots were found whose intensity was influenced by lengthening of the photoperiod; 50 of them, i.e. ca. 5% of the population analysed, were affected before the end of the extension period and were thus potentially related to floral induction. Many of these RNAs were not quantitatively constant during a 24-h cycle in SD. Seven of them oscillated according to the 'light-on' and the 'light-off" signals, among which three seemed to be controlled by phytochrome since their relative amount increased under the standard light conditions but decreased under incandescence even faster than in darkness. The large majority of other RNAs varied with a timing that was not clearly driven by the alternation of light and darkness, indicating that genes related to the biological clock may be especially sensitive to the lengthening of the photoperiod. Furthermore, seven spots were observed that underwent a phase-shift in LD, which consisted, for six of them, of a phase advance of 4-8 h. The steady-state level of CAB mRNA was analysed because the CAB gene family (encoding the chlorophyll a/b-binding proteins of the light-harvesting complexes) is known to be controlled both by the biological clock and phytochrome. In SD, the level was high in the light and low in Abbreviations: CAB = chlorophyll a/b-binding proteins of the light-harvesting complexes; 2D = two-dimensional; LD(s)= long day(s); LDP(s) = long day plant(s); SD(s) = short day(s); SDP(s) = short day plant(s) Correspondence to: C. P6rilleux; FAX: 32 (41) 66 38 31; E-mail: cperille@vm 1.ulg.ac.be darkness; the fluctuation was conducted by a circadian rhythm. When plants were exposed to the inductive LD, the peak of mRNA accumulation that was expected according to the endogenous rhythmicity was abolished, possibly because of the change in light quality during the LD extension.

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“…Genetic and molecular techniques are currently used by many to investigate the transition to flowering in Arabidopsis and its close relative Sinapis alba (white mustard) (Melzer et al 1990; Coen and Meyerowitz 1991 ;Mandel et al 1992;Weigel et al 1992;Lechner and Rau 1993;Shannon and Meeks-Wagner 1993). Numerous Arabidopsis transition-to-flowering mutants are now available (Koornneef et al 1991;Schultz and Haughn 1991;Shannon and Meeks-Wagner 1991;Sung et al 1992;Zagotta et al 1992), some of which flower either early or late, and some of which exhibit morphological changes indicative of an altered transition.…”

Section: Introductionmentioning

confidence: 99%

Bi-directional inflorescence development in Arabidopsis thaliana: Acropetal initiation of flowers and basipetal initiation of paraclades

Hempel

Feldman

1994

Planta

117

163

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In this study we investigated Arabidopsis thaliana (L.) Heynh. inflorescence development by characterizing morphological changes at the shoot apex during the transition to flowering. Sixteen-hour photoperiods were used to synchronously induce flowering in vegetative plants grown for 30 d in non-inductive 8-h photoperiods. During the first inductive cycle, the shoot apical meristem ceased producing leaf primordia and began to produce flower primordia. The differentiation of paraclades (axillary flowering shoots), however, did not occur until after the initiation of multiple flower primordia from the shoot apical meristem. Paraclades were produced by the basipetal activation of buds from the axils of leaf primordia which had been initiated prior to photoperiodic induction. Concurrent with the activation of paraclades was the partial suppression of paraclade-associated leaf primordia, which became bract leaves. The suppression of bract-leaf primordia and the abrupt initiation of flower primordia during the first inductive photoperiod is indicative of a single phase change during the transition to flowering in photoperiodically induced Arabidopsis. Morphogenetic changes characteristic of the transition to flowering in plants grown continuously in 16-h photoperiods were qualitatively equivalent to the changes observed in plants which were photoperiodically induced after 30 d. These results suggest that Arabidopsis has only two phases of development, a vegetative phase and a reproductive phase; and that the production of flower primordia, the differentiation of paraclades from the axils of pre-existing leaf primordia and the elongation of internodes all occur during the reproductive phase.

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A complex pattern of changes in polysomal mRNA populations is evident in the leaves of Arabidopsis thaliana (L.) Heynh. during photoperiodic induction of flowering

Cited by 8 publications

References 31 publications

Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes

Independent modulation of Arabidopsis thaliana polyubiquitin mRNAs in different organs and in response to environmental changes

Changes in gene expression in the leaf of Lolium temulentum L. Ceres during the photoperiodic induction of flowering

Bi-directional inflorescence development in Arabidopsis thaliana: Acropetal initiation of flowers and basipetal initiation of paraclades

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