A Complex Relationship between Visfatin and Resistin and microRNA: An In Vitro Study on Human Chondrocyte Cultures

Abstract

Growing evidence indicates the important role of adipokines and microRNA (miRNA) in osteoarthritis (OA) pathogenesis. The purpose of the present study was to investigate the effect of visfatin and resistin on some miRNA (34a, 140, 146a, 155, 181a, let-7e), metalloproteinases (MMPs), and collagen type II alpha 1 chain (Col2a1) in human OA chondrocytes and in the T/C-28a2 cell line. The implication of nuclear factor (NF)-κB in response to adipokines was also assessed. Chondrocytes were stimulated with visfatin (5 or 10 μg/mL) and resistin (50 or 100 ng/mL) with or without NF-κB inhibitor (BAY-11-7082, 1 μM) for 24 h. Viability and apoptosis were detected by MMT and cytometry, miRNA, MMP-1, MMP-13, and Col2a1 by qRT-PCR and NF-κB activation by immunofluorescence. Visfatin and resistin significantly reduced viability, induced apoptosis, increased miR-34a, miR-155, miR-181a, and miR-let7e, and reduced miR-140 and miR-146a gene expression in OA chondrocytes. MMP-1, MMP-13, and Col2a1 were significantly modulated by treatment of OA chondrocytes with adipokines. Visfatin and resistin significantly increased NF-κB activation, while the co-treatment with BAY11-7082 did not change MMPs or Col2a1 levels beyond that caused by single treatment. Visfatin and resistin regulate the expression levels of some miRNA involved in OA pathogenesis and exert catabolic functions in chondrocytes via the NF-κB pathway. These data confirm the complex relationship between adipokines and miRNA.

Hydrostatic Pressure Regulates Oxidative Stress through microRNA in Human Osteoarthritic Chondrocytes

“…The negative controls were obtained by omitting the primary antibody. Immunoreactivity of β-catenin was semi-quantified as the mean densitometric area of protein signal into the nucleus and into the cytoplasm, by AxioVision 4.6 software measure program (Zeiss, Oberkochen, Germany) [81]. At least 100 chondrocytes from each group were evaluated.…”

Osteoarthritis and Cartilage Regeneration: Focus on Pathophysiology and Molecular Mechanisms

“…In the last decade, numerous microRNAs (miRNAs) have been identified as regulators of chondrocyte signaling pathways and OA initiation and progression. Cheleschi and colleagues investigated the interaction between adipokines and miRNAs known to be involved in OA [35]. The adipokines visfatin and resistin increased apoptosis, MMP-1 and MMP-13 gene expression, while reduced COL2A1 expression in human OA chondrocytes.…”

A comprehensive analysis to understand the mechanism of action of balneotherapy: why, how, and where they can be used? Evidence from in vitro studies performed on human and animal samples

“…In vitro studies were normally used to evaluate the effects and/or the mechanism of action of mechanical (such as hydrostatic pressure, mechanical compression, ultrasound, magnetic or electromagnetic fields) or chemical factors (cytokine, growth factors, adipokines, drugs, mineral elements, etc.) on cell or tissue morphology and metabolism (Cheleschi et al 2015;Cheleschi et al 2018;Cheleschi et al 2019a;Cheleschi et al 2019b;Collodel et al 2013;Fioravanti et al 2010;Kloesch et al 2011;Marrazzo et al 2019;Shams et al 2018).…”