A composite microbial agent containing bacterial and fungal species: Optimization of the preparation process, analysis of characteristics, and use in the purification for volatile organic compounds

Cheng, Zhuowei; Lu, Lichao; Kennes, Christian; Ye, Jiexu; Yu, Jiaguo; Chen, Dongzhi; Chen, Jianmeng

doi:10.1016/j.biortech.2016.07.001

Cited by 17 publications

(9 citation statements)

References 33 publications

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“…Agent M1 increased the abundance of Firmicutes during the initial thermophilic period, promoting the decomposition of organic matter. In all four groups Actinobacteria showed increasing trend, consistent with the results of Wang et al [10], Cheng et al [15], and Zhao et al [13] During the composting process, the steady rise in Actinobacteria highlighted their degradation functions during the thermophilic and cooling phases, possibly due to their thermostability and the direct correlation with lignocellulose and humus contents [49-50]. Zimmermann et al [51] found that a diverse range of mesophilic and thermophilic actinomycetes in Actinobacteria could degrade lignocelluloses.…”

Section: Discussionsupporting

confidence: 90%

“…It is an environmental and economical way for treating garden wastes. While, applying the poor biodegradability of microbial community will lead to low composting efficiency [7,15]. In order to fasten compost maturation process and improve the quality of compost, adding exogenous microorganisms is an effective way.…”

Section: Discussionmentioning

confidence: 99%

“…Understanding such microbial interactions and dynamics could reveal the mechanisms driving material transformation and the overall process of compost maturation. Most studies have concluded that the effect of the addition of a single strain to compost is lower than that of mixed strains, because the degradation of organic matter requires a combination of many enzymes secreted by different microbes [14-15]. Nevertheless, few studies have added different microbial agents to individual compost piles, especially carefully screened agents, to compare the effects with natural compost on microbial communities.…”

Section: Introductionmentioning

confidence: 99%

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Effects of a novel thermophilic cellulose-degrading agent on the quality of compost and change in microbial community of garden waste

Fan

Jia

et al. 2019

Preprint

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12Knowledge about the microbial communities in composting has advanced, but definitive 13 knowledge concerning the application of actinomycetal communities in garden waste 14 composting is still lacking. In this study, we compared the effects of amending compost with 15 mixed agent M1 (five high-degradability strains) and other agents on the physicochemical 16 indices and microbial community succession. The results showed that Pile A (only applying 17 M1), exhibited a pH closer to neutral, the complete degradation of organic matter, and the 18 highest remaining levels of nitrogen, phosphorus, and potassium. The seed germination rate, 19 root length, and seed germination index values were significantly higher in piles amended 20 with M1 and/or commercially available agents than in piles without exogenous microbial 21 agents. Analyzing the microbial communities, these treatments were dominated by 22 Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes during composting. The amount 23 of Streptomyces was negatively correlated with the carbon/nitrogen ratio and positively 24 correlated with total phosphorus and total potassium. Adding M1 increased microbial 25 diversity, and the dominant microbial communities at the end of composting were similar to 26 those found in the commercial microbial inoculum. Overall, agent M1 can shorten the 27 composting process and increase the extent of degradation. This research provides additional 28 insights into the potential function of Actinomycetes in compost ecology. 29 30 31 thermophilic cellulose-degrading bacteria 32 33 34 35 36 37 38 39 40 41 42 43 45 Waste management is one of the main issues affecting all developing nations [1]. In 46 China, with the acceleration of urbanization, the amount of green area coverage is increasing, 47 but tons of organic solid waste are produced with no means for effective decomposition, 48 representing added stress to the environment [2]. The main components of garden waste, 49 lignin and cellulose, are recalcitrant to degradation, leaving only landfilling and direct 50 burning as traditional disposal methods. Traditional landfilling and incineration methods not 51 only use land resources, but also cause other problems, such as dust particle production, soil 52 and groundwater contamination, pathogenic bacteria growth, and air pollution [3]. To lessen 53 the effects of garden waste disposal on the environment and mitigate environmental pollution, 54 biological composting employs microbial action in waste piles to transform organic solid 55 waste into stable humus and organic fertilizer. Biological composting can directly transform 56 large amounts of organic waste for compost production; however, the unreliability of the 57 quantity and biodegradability of the indigenous functional microbial community in compost 58 often leads to low composting efficiency and undesirable compost quality [4]. 59 Many studies have shown that inoculation with exogenous microorganisms is an 60 effective method for the biodegradation of organic matter a...

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of a novel thermophilic cellulose-degrading agent on the quality of compost and change in microbial community of garden waste

Fan

Jia

et al. 2019

Preprint

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“…There are very few studies on the bacterial population composition in the full-scale biofilter units used for the treatment of waste gas emissions [21][22][23]. Inoculation of specialized (adapted) microorganisms in bioreactors may also be useful in some applications [24], as prolonged sustainability of microbial activity is a significant problem.…”

Section: Introductionmentioning

confidence: 99%

Identifying Bacterial Communities in a Full-Scale Wood Chip Biofilter

Çelikten¹,

Saral²,

Kuzu³

2019

Pol. J. Environ. Stud.

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Although biofilters have an important role in the purification of VOC mixtures or inorganic waste gas compounds and despite their widespread use, the microbial community characterization is not fully known, particularly in full-scale biofiltering processes. Maintaining microbial activity and sustainability are major factors in biological processes such as biofilters, in which the contaminating compounds are catalyzed by the microbial community. In this study, bacterial diversity was investigated in a full-scale biofilter unit, which is operated to treat odorous gases, collected from a composting process. Bacterial strains composition was determined by the DNA isolation of samples taken from wood chips. The wood chips were processed by amplification of 16S rDNA by PCR (polymerase chain reaction) and sequencing. The determined nucleotide and translated amino acid sequences were compared with those deposited in the National Center for Biotechnology Information (NCBI) database by a BLASTN search. Bacillaceae (Solibacillus silvestris, Bacillus licheniformis, Bacillus cereus, Bacillus paralicheniformis) and Enterobacteriaceae (Enterobacter cloacae, Enterobacter ludwigii, Serratia marcescens) dominated the bacterial family in the packing material by 53% and 47%, respectively.

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“…Stabilization of anaerobic bacteria that are capable of assimilating single carbon gases, such as C . ljungdahlii , and other clostridia, could prove to be highly beneficial in developing bioprocess-oriented large scale gas treatment technologies [5–7]. …”

Section: Introductionmentioning

confidence: 99%

A technique for lyopreservation of Clostridium ljungdahlii in a biocomposite matrix for CO absorption

Schulte¹,

Solocinski²,

Wang³

et al. 2017

PLoS ONE

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A system capable of biocatalytic conversion of distributed sources of single carbon gases such as carbon monoxide into hydrocarbons can be highly beneficial for developing commercially viable biotechnology applications in alternative energy. Several anaerobic bacterial strains can be used for such conversion. The anaerobic carbon monoxide-fixing bacteria Clostridium ljungdahlii OTA1 is a model CO assimilating microorganism that currently requires cryogenic temperature for storage of the viable strains. If these organisms can be stabilized and concentrated in thin films in advanced porous materials, it will enable development of high gas fraction, biocomposite absorbers with elevated carbon monoxide (CO) mass transfer rate, that require minimal power input and liquid, and demonstrate elevated substrate consumption rate compared to conventional suspended cell bioreactors. We report development of a technique for dry-stabilization of C. ljungdahlii OTA1 on a paper biocomposite. Bacterial samples coated onto paper were desiccated in the presence of trehalose using convective drying and stored at 4°C. Optimal dryness was ~1g H2O per gram of dry weight (gDW). CO uptake directly following biocomposite rehydration steadily increases over time indicating immediate cellular metabolic recovery. A high-resolution Raman microspectroscopic hyperspectral imaging technique was employed to spatially quantify the residual moisture content. We have demonstrated for the first time that convectively dried and stored C. ljungdahlii strains were stabilized in a desiccated state for over 38 days without a loss in CO absorbing reactivity. The Raman hyperspectral imaging technique described here is a non-invasive characterization tool to support development of dry-stabilization techniques for microorganisms on inexpensive porous support materials. The present study successfully extends and implements the principles of dry-stabilization for preservation of strictly anaerobic bacteria as an alternative to lyophilization or spray drying that could enable centralized biocomposite biocatalyst fabrication and decentralized bioprocessing of CO to liquid fuels or chemicals.

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A composite microbial agent containing bacterial and fungal species: Optimization of the preparation process, analysis of characteristics, and use in the purification for volatile organic compounds

Cited by 17 publications

References 33 publications

Effects of a novel thermophilic cellulose-degrading agent on the quality of compost and change in microbial community of garden waste

Effects of a novel thermophilic cellulose-degrading agent on the quality of compost and change in microbial community of garden waste

Identifying Bacterial Communities in a Full-Scale Wood Chip Biofilter

A technique for lyopreservation of Clostridium ljungdahlii in a biocomposite matrix for CO absorption

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