A Composite Regulatory Element in the First Intron of the Estrogen-Responsive Very Low Density Apolipoprotein II Gene

Shuler, Franklin D.; Chu, William W.; Wang, Shiyi; Evans, Morgan W.

doi:10.1089/dna.1998.17.689

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…The ␤-actin promoter was not affected by E 2 in the cotransfection assays. The apoVLDLII promoter was strongly induced, which is consistent with in vivo observations (39). The data generated in the cotransfection model were reproducible in a large number of experiments, demonstrating that the decrease in HL expression caused by E 2 is due to transcriptional repression.…”

Section: Discussionsupporting

confidence: 87%

“…1C). The chicken apoVLDLII promoter was also included as a control because the gene is highly inducible by estrogen in vivo (39). Basal activity of the apoVLDLII promoter was similar to that of the HL promoter, but it was increased approximately 5-fold in the presence of E 2 (Fig.…”

Section: Estrogen Represses Hl Gene Transcription In Transient Cotransfection Assays Of Hepg2 Cellsmentioning

confidence: 97%

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Estrogen receptor-mediated repression of human hepatic lipase gene transcription

Jones

Schmidt

Pickard

et al. 2002

Journal of Lipid Research

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Estrogen replacement therapy in women decreases hepatic lipase (HL) activity, which may account for the associated increase in HDL cholesterol. To investigate whether estrogen decreases HL transcription, transient cotransfection assays with HL promoter and estrogen receptor-␣ (ER ␣ ) expression constructs were performed in HepG2 cells. 17 ␤ -estradiol (E 2 ) decreased transcription driven by the ؊ 1557/ ؉ 41 human HL promoter by up to 50% at 10 ؊ 7 M. Mutation of ER ␣ by deletion of its transactivation domains or ligand-binding domain eliminated E 2 -induced repression of the promoter, whereas deletion of the DNA-binding domain of ER ␣ resulted in a 7-fold activation by E 2 . The E 2induced repression was maintained after mutation of a potential estrogen-response element in the promoter. The region of estrogen responsiveness was localized to ؊ 1557/ ؊ 1175 of the HL promoter by deletion analysis. Mutation of an AP-1 site at ؊ 1493 resulted in a partial loss of E 2induced repression, similar to that caused by deletion of nucleotides ؊ 1557 to ؊ 1366. Gel shift assays with nuclear extracts from E 2 -treated HepG2 cells stably expressing ER ␣ demonstrated an increase in binding to an AP-1 consensus oligonucleotide. The AP-1 activator, phorbol 12-myristate 13acetate, inhibited the HL promoter by greater than 50%. Collectively, the data suggest that estrogen represses the transcription of the HL gene, possibly through an AP-1 pathway. -

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Section: Discussionsupporting

confidence: 87%

Section: Estrogen Represses Hl Gene Transcription In Transient Cotransfection Assays Of Hepg2 Cellsmentioning

confidence: 97%

Estrogen receptor-mediated repression of human hepatic lipase gene transcription

Jones

Schmidt

Pickard

et al. 2002

Journal of Lipid Research

View full text Add to dashboard Cite

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“…I: aiso contains sequences within its first intron that increase transcription (Schippers et al,1994). By using in uitro DNAseI footprinting, 6 protein-binding sites were revealed throughout the first intron and those protein-binding sites affect expression of apoVLDL-I1 gene and other correlative gene (Shuler et al, 1998). However, up t o now, research about SNPs of the apoVLDL-I1 gene in White Muscovy have not been reported.…”

mentioning

confidence: 99%

Polymorphism in Intron-1 of ApoVLDL-II Gene and its Association with Production Traits and Biochemical Levels in White Muscovy (Cairina moschata)

Zhang

Chen²,

Xu³

et al. 2010

Journal of Applied Animal Research

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Thyroid Hormone Regulates Carnitine Palmitoyltransferase Iα Gene Expression through Elements in the Promoter and First Intron

Jansen¹,

Cook

Song

et al. 2000

Journal of Biological Chemistry

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A Composite Regulatory Element in the First Intron of the Estrogen-Responsive Very Low Density Apolipoprotein II Gene

Cited by 6 publications

References 35 publications

Estrogen receptor-mediated repression of human hepatic lipase gene transcription

Estrogen receptor-mediated repression of human hepatic lipase gene transcription

Polymorphism in Intron-1 of ApoVLDL-II Gene and its Association with Production Traits and Biochemical Levels in White Muscovy (Cairina moschata)

Thyroid Hormone Regulates Carnitine Palmitoyltransferase Iα Gene Expression through Elements in the Promoter and First Intron

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