A compounds annotation strategy using targeted molecular networking for offline two-dimensional liquid chromatography-mass spectrometry analysis: Yupingfeng as a case study

Zhu, Haodong; He, Liangliang; Duan, Huifang; Chen, Jiali; Xiao, Qiang; Lin, Pei; Qin, Z. H.; Dai, Yi; Wu, Wanying; Hu, Linlin; Yao, Zhihong

doi:10.1016/j.chroma.2023.464045

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article3

Book1

Relationship

Self Cite0

Independent4

Authors

Journals

Cited by 4 publications

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Metabolic fate of the natural anticancer agent cucurbitacin B: an LC–MS/MS-enabled profiling of its major phase I and II conjugates in vivo

Liu,

Xu,

Meng

et al. 2024

Anal Bioanal Chem

View full text Add to dashboard Cite

Metabolic fate of the natural anticancer agent cucurbitacin B: an LC–MS/MS-enabled profiling of its major phase I and II conjugates in vivo

Liu,

Xu,

Meng

et al. 2024

Anal Bioanal Chem

View full text Add to dashboard Cite

Innovative orthogonal two-dimensional reversed-phase liquid chromatography × supercritical fluid chromatography with a phenyl/tetrazole stationary phase for the preparative isolation of diarylheptanoids

Dang,

Tong,

Wang

et al. 2024

Journal of Chromatography A

View full text Add to dashboard Cite

Systematic Characterization of Sesquiterpenes from Dendrobium nobile through Offline Two-Dimensional Chromatography Tandem Mass Spectrometry and Target Isolation

Wang,

Li,

Yao

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Dendrobium nobile is a species of the genus Dendrobium that can be used as both a medicinal herb and healthy food. The sesquiterpenes in D. nobile have attracted extensive attention in recent years. In this study, Amide × RP offline two-dimensional chromatography separation tandem high-resolution mass spectrometry combined with GNPS (Global Natural Product Social Molecular Networking) was developed for the characterization of sesquiterpenes in D. nobile. After first-dimensional amide separation, the 70% ethanol extract of D. nobile was divided into 40 fractions, which were analyzed by second-dimensional reverse-phase system separation and LTQ-Orbitrap detection. The raw data was imported into the GNPS, resulting in the efficient clustering of similar substances. Finally, 594 sesquiterpene compounds were characterized, and 25 compounds were isolated based on molecular network analysis, including six new compounds. In vitro bioassays, the isolated compounds decreased NO production in the LPS-induced microglial BV-2 cell model and the content of MDA in PC12 cells, demonstrating neuroprotective activity. These findings unraveled the underlying material and provided valuable insights into the quality control of D. nobile.

show abstract

A compounds annotation strategy using targeted molecular networking for offline two-dimensional liquid chromatography-mass spectrometry analysis: Yupingfeng as a case study

Cited by 4 publications

References 42 publications

Metabolic fate of the natural anticancer agent cucurbitacin B: an LC–MS/MS-enabled profiling of its major phase I and II conjugates in vivo

Metabolic fate of the natural anticancer agent cucurbitacin B: an LC–MS/MS-enabled profiling of its major phase I and II conjugates in vivo

Innovative orthogonal two-dimensional reversed-phase liquid chromatography × supercritical fluid chromatography with a phenyl/tetrazole stationary phase for the preparative isolation of diarylheptanoids

Systematic Characterization of Sesquiterpenes from Dendrobium nobile through Offline Two-Dimensional Chromatography Tandem Mass Spectrometry and Target Isolation

Contact Info

Product

Resources

About