A comprehensive feasibility study of effectiveness and environmental impact of PAH bioremediation using an indigenous microbial degrader consortium and a novel strain Stenotrophomonas maltophilia CPHE1 isolated from an industrial polluted soil

Lara-Moreno, Alba; Morillo, E.; Merchán, Francisco; Villaverde, Jaime

doi:10.1016/j.jenvman.2021.112512

Cited by 27 publications

(18 citation statements)

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“…The PHE-degrading microorganism selected, S. indicatrix CPHE1, corresponds to S. maltophilia CPHE1 (NCBI number: MT138842) published by Lara-Moreno et al (2021) . Subsequent studies indicated greater phylogenetic closeness with the species S. indicatrix rather than S. maltophilia ( Lara-Moreno et al, 2023 ) .…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, bacterial strains from Stenotrophomonas genus have been described as PHE degraders ( Kumari et al, 2017 ; Elufisan et al, 2020 ), such as S. maltophilia ( Gao et al, 2013 ; Zafra et al, 2014 ; Xiao et al, 2021 ). However, as far as we know, the study of Lara-Moreno et al (2021) was the first which demonstrated the ability of S. indicatrix to degrade PHE in solution. Stenotrophomonas indicatrix is a Gram-negative species that can be isolated from sunflower roots ( Adeleke et al, 2021 ), soils ( Lara-Moreno et al, 2021 ; Lozano-Andrade et al, 2021 ), oligotrophic water ponds ( Friedrich et al, 2021 ), or from food-associated sources ( Weber et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, as far as we know, the study of Lara-Moreno et al (2021) was the first which demonstrated the ability of S. indicatrix to degrade PHE in solution. Stenotrophomonas indicatrix is a Gram-negative species that can be isolated from sunflower roots ( Adeleke et al, 2021 ), soils ( Lara-Moreno et al, 2021 ; Lozano-Andrade et al, 2021 ), oligotrophic water ponds ( Friedrich et al, 2021 ), or from food-associated sources ( Weber et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…It has been recommended as the most suitable alternative to be used in bioremediation ( Nwankwegu et al, 2022 ), using bacterial strains isolated from large-scale polluted areas where endogenous microbiota is generally adapted ( Mrozik and Piotrowska-Seget, 2010 ). For this reason, in the present study, a bacterial strain isolated in our lab from highly contaminated industrial soil was selected for its capacity to degrade and mineralize PHE in an aqueous solution ( Lara-Moreno et al, 2021 ). In the previous paper, the isolated bacterial strain was classified as Stenotrophomonas maltophilia CPHE1 (NCBI number: MT138842), but later, a more in-depth genetic study revealed that its classification corresponded to S. indicatrix ( Lara-Moreno et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Genome analysis for the identification of genes involved in phenanthrene biodegradation pathway in Stenotrophomonas indicatrix CPHE1. Phenanthrene mineralization in soils assisted by integrated approaches

Lara-Moreno

Merchán

Morillo

et al. 2023

Front. Bioeng. Biotechnol.

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Phenanthrene (PHE) is a highly toxic compound, widely present in soils. For this reason, it is essential to remove PHE from the environment. Stenotrophomonas indicatrix CPHE1 was isolated from an industrial soil contaminated by polycyclic aromatic hydrocarbons (PAHs) and was sequenced to identify the PHE degrading genes. Dioxygenase, monooxygenase, and dehydrogenase gene products annotated in S. indicatrix CPHE1 genome were clustered into different trees with reference proteins. Moreover, S. indicatrix CPHE1 whole-genome sequences were compared to genes of PAHs-degrading bacteria retrieved from databases and literature. On these basis, reverse transcriptase-polymerase chain reaction (RT-PCR) analysis pointed out that cysteine dioxygenase (cysDO), biphenyl-2,3-diol 1,2-dioxygenase (bphC), and aldolase hydratase (phdG) were expressed only in the presence of PHE. Therefore, different techniques have been designed to improve the PHE mineralization process in five PHE artificially contaminated soils (50 mg kg−1), including biostimulation, adding a nutrient solution (NS), bioaugmentation, inoculating S. indicatrix CPHE1 which was selected for its PHE-degrading genes, and the use of 2-hydroxypropyl-β-cyclodextrin (HPBCD) as a bioavailability enhancer. High percentages of PHE mineralization were achieved for the studied soils. Depending on the soil, different treatments resulted to be successful; in the case of a clay loam soil, the best strategy was the inoculation of S. indicatrix CPHE1 and NS (59.9% mineralized after 120 days). In sandy soils (CR and R soils) the highest percentage of mineralization was achieved in presence of HPBCD and NS (87.3% and 61.3%, respectively). However, the combination of CPHE1 strain, HPBCD, and NS showed to be the most efficient strategy for sandy and sandy loam soils (LL and ALC soils showed 35% and 74.6%, respectively). The results indicated a high degree of correlation between gene expression and the rates of mineralization.

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Section: Methodsmentioning

confidence: 99%