A comprehensive gas chromatographic scheme for the estimation of antiepileptic drugs

Gardner‐Thorpe, Christopher; Parsonage, M. J.; Smethurst, P. F.; Toothill, C.

doi:10.1016/0009-8981(72)90181-7

Cited by 28 publications

(10 citation statements)

References 10 publications

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“…Specificity may, however, be improved by the use of other detection procedures ( Gardner-Thorpe et al 1971). Although only four drugs have been examined in this investigation, it has been shown that the GC system can be applied to 18 antiepileptic drugs (Gardner- Thorpe et al 1972). This is also true with regard to the range of drug concentrations, all obtained with GC, which are discussed below with respect to each drug.…”

Section: Thin-layer and Gas Chroniatographymentioning

confidence: 94%

“…Plasma concentrations of four drugs were estimated by thin-layer chromatography (Gardner- Thorpe et al 1971) and gas chromatography (Gardner- Thorpe et al 1972). Plasma concentrations of four drugs were estimated by thin-layer chromatography (Gardner- Thorpe et al 1971) and gas chromatography (Gardner- Thorpe et al 1972).…”

Section: Methodsmentioning

confidence: 99%

“…These have already been reviewed briefly (Gardner-Thorpe et al 1971, 1972. These have already been reviewed briefly (Gardner-Thorpe et al 1971, 1972.…”

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confidence: 99%

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ANTIEPILEPTIC DRUG CONCENTRATIONS IN PLASMA:Clinical Evaluation of 321 Estimations in 237 Unselected Patients

Gardner‐Thorpe¹,

Parsonage²,

Smethurst³

et al. 1972

Acta Neurologica Scandinavica

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Two chromatographic methods of estimating plasma concentrations of antiepileptic drugs, designed by the authors and described elsewhere, have been used to examine 321 samples of plasma. The methods have bcen shown to be simple and to fit satisfactorily into a busy laboratory programme. Results obtained with both methods have been found to agree with those of other workers who used a wide variety of techniques. Ranges of plasma concentrations of four commonly-used drugs have bccn established. Plasma concentrations do not correlate well with drug doses in individual patients. Negligible plasma concentrations have been found in some patients. Possihle metahnlic interactions have been revealed.14'

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Section: Thin-layer and Gas Chroniatographymentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

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ANTIEPILEPTIC DRUG CONCENTRATIONS IN PLASMA:Clinical Evaluation of 321 Estimations in 237 Unselected Patients

Gardner‐Thorpe¹,

Parsonage²,

Smethurst³

et al. 1972

Acta Neurologica Scandinavica

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“…Gardner-Thorpe el al. (2) used TLC and examination under UV light to estimate the presence of some anticonvulsant succinimides in blood.…”

Section: Methsuximide (N2-dimethyl-2-phenylsuccinimide I)mentioning

confidence: 99%

NMR Analysis of Pharmaceuticals X: Determination of Methsuximide and Phensuximide in Capsules

Turczan

Goldwitz

1973

Journal of Pharmaceutical Sciences

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“…The determination of beclamide in body fluids is hampered by the low molar-absorptivity (e = 190) of the compound and like many amides, it presents difficulty when gas chromatography is used for analysis. A gas chromatographic method for the estimation of plasma concentrations of some anti-epileptic drugs has previously been described by Gardner-Thorpe, Parsonage, Smethurst & Toothill (1972) but the method has generally proved to lack sensitivity and reproducibility in the determination of beclamide.In an attempt to quantify the amount of beclamide in solvent extracts of plasma using gas chromatography, most of the common stationary phases (e.g. SE 30, OV-1, 0-17, Apiezon-L and OV-225) were found to give two peaks on the chromatogram.…”

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A simple method for the determination of beclamide in plasma by gas chromatography.

Jones¹,

Leach²,

Luscombe³

et al. 1975

Brit J Clinical Pharma

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. The determination of beclamide in body fluids is hampered by the low molar-absorptivity (e = 190) of the compound and like many amides, it presents difficulty when gas chromatography is used for analysis. A gas chromatographic method for the estimation of plasma concentrations of some anti-epileptic drugs has previously been described by Gardner-Thorpe, Parsonage, Smethurst & Toothill (1972) but the method has generally proved to lack sensitivity and reproducibility in the determination of beclamide.In an attempt to quantify the amount of beclamide in solvent extracts of plasma using gas chromatography, most of the common stationary phases (e.g. SE 30, OV-1, 0-17, Apiezon-L and OV-225) were found to give two peaks on the chromatogram. Although this enabled semiquantitative data to be obtained, it was apparent that further development of the method was required to ensure greater analytical precision. Following the use of a variety of stationary phases at different loadings, it was found that a polyamide stationary phase (Poly-A 103) gave single symmetrical peaks and therefore extraction of beclamide from plasma was followed by gas chromatography on this stationary phase.Plasma (2 ml) and 1.0 M phosphate buffer (I ml) at pH 7.4 were introduced into a 50 ml glass stoppered centrifuge tube, followed by 0.4 ml of the internal standard (10 mg/100 ml barbitone). To each tube, chloroform (45 ml) was added and the stoppered tubes were mixed on a rotary mixer for 0.5 hours. After centrifugation, the aqueous layer was removed as completely as possible and traces of water were removed with anhydrous sodium sulphate (about 0.5 g). The solvent was decanted into a beaker and evaporated to dryness in a vacumn desiccator or on a low heat. The residue was dissolved in a little chloroform and washed into a small tapered tube. After evaporation to dryness, carbon disulphide (0.2 ml) was added and 1-2 ,ul was injected into a Pye senres 104 gas chromatograph fitted with a flame ionization detector. Columns were 1.5 m borosilicate glass with an outside

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A comprehensive gas chromatographic scheme for the estimation of antiepileptic drugs

Cited by 28 publications

References 10 publications

ANTIEPILEPTIC DRUG CONCENTRATIONS IN PLASMA:Clinical Evaluation of 321 Estimations in 237 Unselected Patients

ANTIEPILEPTIC DRUG CONCENTRATIONS IN PLASMA:Clinical Evaluation of 321 Estimations in 237 Unselected Patients

NMR Analysis of Pharmaceuticals X: Determination of Methsuximide and Phensuximide in Capsules

A simple method for the determination of beclamide in plasma by gas chromatography.

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