A comprehensive quality control evaluation for standard decoction of Smilax glabra Roxb based on HPLC-MS-UV/CAD methods combined with chemometrics analysis and network pharmacology

Shi, Wenqing; Wu, Zhenhua; Wu, Jiangping; Jia, Mengqi; Yang, Chen; Feng, Jianfang; Lou, Yuefen; Fan, Guorong

doi:10.1016/j.foodchem.2022.135371

Cited by 17 publications

(11 citation statements)

References 34 publications

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“…Among them, the isopentenyl and ketone groups are the main components that ensure the pharmacological activities of NIA and neoastilbin, such as lowering uric acid, and anti-inflammatory and analgesic activities. [ 43 ] Calycosin is composed of a flavonoid core and multiple hydroxyl and methoxy groups. The benzene ring and two isoprene units that constitute the flavonoid core also ensure multiple pharmacological activities of calycosin.…”

Section: Resultsmentioning

confidence: 99%

Exploring effect of herbal monomers in treating gouty arthritis based on nuclear factor-kappa B signaling: A review

Guo,

Ye,

Tang

et al. 2024

Medicine

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Gouty arthritis (GA) is an inflammatory disease caused by disorders of the purine metabolism. Although increasing number of drugs have been used to treat GA with the deepening of relevant research, GA still cannot be cured by simple drug therapy. The nuclear factor-kappa B (NF-κB) signaling pathway plays a key role in the pathogenesis of GA. A considerable number of Chinese herbal medicines have emerged as new drugs for the treatment of GA. This article collected relevant research on traditional Chinese medicine monomers in the treatment of GA using NF-κB, GA, etc. as keywords; and conducted a systematic search of relevant published articles using the PubMed database. In this study, we analyzed the therapeutic effects of traditional Chinese medicine monomers on GA in the existing literature through in vivo and in vitro experiments using animal and cell models. Based on this review, we believe that traditional Chinese medicine monomers that can treat GA through the NF-κB signaling pathway are potential new drug development targets. This study provides research ideas for the development and application of new drugs for GA.

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Section: Resultsmentioning

confidence: 99%

Exploring effect of herbal monomers in treating gouty arthritis based on nuclear factor-kappa B signaling: A review

Guo,

Ye,

Tang

et al. 2024

Medicine

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“…Chemometrics, also known as chemical statistics, is a branch of chemistry that combines mathematics, statistics, computer science, and chemistry, which the most important feature of chemistry is the introduction of multivariate analysis methods into chemical research and the multivariate processing and analysis of chemical measurement data. Chemometrics include measurement tests, chemical pattern recognition, regression analysis, and multivariate correction [ 15 , 16 ]. PCA is an unsupervised pattern recognition analysis which uses the idea of reducing the dimension of the data matrix to convert the original indicators into several comprehensive indicators through a linear transformation under the premise of losing a small amount of information, so as to simplify datasets and visualize differences between samples [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics

Wu,

Lv,

Tang

et al. 2024

International Journal of Analytical Chemistry

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Radix Dipsaci (RD) is the dry root of the Dipsacus asper Wall. ex DC., which is commonly used for tonifying the kidney and strengthening bone. The purpose of this study was to analyze the difference between raw and salt-processed RD from the chemical composition comprehensively. The fingerprints of raw and salt-processed RD were established by HPLC-DAD to determine the contents of loganin (LN), asperosaponin VI (AVI), caffeic acid (CaA), dipsanoside A (DA), dipsanoside B (DB), chlorogenic acid (CA), loganic acid (LA), isochlorogenic acid A (IA), isochlorogenic acid B (IB), and isochlorogenic acid C (IC). The results showed that after processing with salt, the components with increased contents were LA, CaA, DA, and AVI, and the components with decreased contents were CA, LN, IB, IA, IC, and DB. Then, the chemometric methods such as principal component analysis (PCA) and fisher discriminant analysis (FDA) were used to evaluate the quality of raw and salt-processed RD. In the classification of raw and salt-processed RD, the order of importance of each chemical component was LA > DB > IA > IC > IB > LN > CA > DA > AVI > CaA. These integrated methods successfully assessed the quality of raw and salt-processed RD, which will provide guidance for the development of RD as a clinical medication.

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“…(SGR) is a traditional Chinese botanical drug, referred to as tufuling in Chinese medicine. Approximately 190 SGR compounds have been isolated and identified, including phenylpropanoids, flavonoids, sterols, organic acids, naphtha, and seven other categories ( Shi et al, 2023 ). SGR has obvious pharmacological activity, especially in anti-inflammation and treating immune system diseases and tumors ( Abaidullah et al, 2023 ; Zhao et al, 2020 ; Wu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the molecular mechanism of Smilax glabra Roxb. in treating hypertension based on proteomics and bioinformatics

Yang,

Qian,

Yang

et al. 2024

Front. Pharmacol.

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BackgroundSmilax glabra Roxb. (named tufuling in Chinese, SGR) has both medicinal and edible value. SGR has obvious pharmacological activity, especially in anti-inflammation and treating immune system diseases. This study investigated differential protein expression and its relationship with immune infiltration in hypertension treated with SGR using proteomics and bioinformatics.MethodsN-Nitro L-arginine methyl ester (L-NAME) was used to replicate the hypertension model, with SGR administered by gavage for 4 weeks, and the systolic and diastolic blood pressure in each group of rats was measured using the tail-cuff method every 7 days. Furthermore, enzyme-linked immunosorbent assay (ELISA) was used to determine the serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) expressions in each group, followed by the detection of protein expression in rat liver samples using the tandem mass tag (TMT) technique. Additionally, hub targets were output using Cytoscape 3.9.1 software, and ALDH2 expression in the liver and serum in each group of rats was detected by ELISA. Moreover, R4.3.0 software was used to evaluate the relationship between acetaldehyde dehydrogenase 2 (ALDH2) and immune cells, and ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) was performed to identify the components of SGR. Furthermore, the association between components of SGR and ALDH2 was analyzed with molecular docking and LigPlot1.4.5 software.ResultsCompared with the model group (L-NAME), SGR at high and medium doses reduced systolic and diastolic blood pressure while reducing TC, TG, and LDL-C levels and increasing HDL-C levels in hypertensive rats (p < 0.05). Moreover, 92 differentially expressed proteins (DEPs) were identified using TMT. These DEPs participated in peroxisome functioning, fatty acid degradation, and other signaling pathways, with ALDH2 being the core target and correlated with various immune cells. In addition, 18 components were determined in SGR, with 8 compounds binding to ALDH2. Molecular docking was performed to confirm that SGR played a role in hypertension based on the combined action of multiple components.ConclusionIn conclusion, SGR has an antihypertensive effect on L-NAME-induced hypertension, with ALDH2 as its hub target. SGR may regulate neutrophil, regulatory T cell, and other cells’ infiltration by targeting ALDH2, thereby contributing to the treatment of hypertension.

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A comprehensive quality control evaluation for standard decoction of Smilax glabra Roxb based on HPLC-MS-UV/CAD methods combined with chemometrics analysis and network pharmacology

Cited by 17 publications

References 34 publications

Exploring effect of herbal monomers in treating gouty arthritis based on nuclear factor-kappa B signaling: A review

Exploring effect of herbal monomers in treating gouty arthritis based on nuclear factor-kappa B signaling: A review

Analysis of Quality Differences in Radix Dipsaci before and after Processing with Salt Based on Quantitative Control of HPLC Multi-Indicator Components Combined with Chemometrics

Investigation of the molecular mechanism of Smilax glabra Roxb. in treating hypertension based on proteomics and bioinformatics

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