A Comprehensive Quality Evaluation Method Based on C30-HPLC and an Analytic Hierarchy Process for the Chinese Herbal Formula, Erzhiwan

Abstract

The quantitative analysis of multiple indexes remains an important quality evaluation method of traditional Chinese medicine (TCM) herbal formulas. The Chinese Pharmacopoeia 2015 only stipulates the content of a single component, specnuezhenide, in Erzhiwan composed of the Fructus Ligustri Lucidi (FLL) powder and aqueous extracts of Herba Ecliptae (HE). To generalize the intrinsic quality of Erzhiwan, a novel C30-HPLC method with good precision, accuracy, and reproducibility was developed for the simultaneous determination of six compounds, including two isomers, and then an analytic hierarchy process was further applied to integrate and discriminate the quality of four samples prepared via different methods. The results of the analysis were in agreement with the antioxidant tests in vitro. This comprehensive strategy could provide a reference and suggestions for the improvement of the quality evaluation method of TCM herbal formulas.

Enrichment of flavonoid‐rich extract from Bidens bipinnata L. by macroporous resin using response surface methodology, UHPLC–Q‐TOF MS/MS‐assisted characterization and comprehensive evaluation of its bioactivities by analytical hierarchy process

“…It aids in decision‐making by prioritizing factors to be considered when making decisions, especially in complex situations where several factors are involved. Recently, the AHP method has been widely used in evaluation of optimization of extraction processes of bioactive compounds, assessment of diseases risk and quality research in Chinese medicine, as well as comprehensive comparison of multi‐bioactivities (Ma, Bao, Meng, & Wang, 2015; Nie & Yao, 2018). In this study, the goal of the model was set as evaluation of the multi‐bioactivities of BBCE and BBTF.…”

A Network-Based Approach to Explore the Mechanism and Bioactive Compounds of Erzhi Pill against Metabolic Dysfunction-Associated Fatty Liver Disease

“…This is illustrated in Table 1 . Some compounds that were removed after ADME screening have been identified as the main compounds of EZP in previous studies [ 33 , 34 ]. Therefore, oleanolic acid, salidroside, and specnuezhenide were identified as bioactive compounds.…”

Systematic Elucidation of the Potential Mechanism of Erzhi Pill against Drug‐Induced Liver Injury via Network Pharmacology Approach

“…After ADME screening by OB ≥ 30% and DL ≥ 0.18, 20 compounds, 13 compounds from LLF and 9 compounds from EH with two repeated compounds (luteolin and quercetin), were identified as bioactive compounds of EZP (Figures 2(a) and 2(b)). Furthermore, some compounds (oleanolic acid, salidroside, and specnuezhenide) were removed by ADME screening, but these ingredients were identified as the main constituents of EZP in previous studies [17,18]. At last, 23 compounds were identified as potential bioactive molecules for further study.…”