A Comprehensive Review of Drugs Determined by Spectrophotometry using Vanillin as a Chromogenic Reagent in the Past Decade

Abstract

Many analytical techniques have been used in quality control, such as spectrophotometry, spectrofluorimetry, HPLC, and other hyphenated techniques. Among them, spectrophotometry is considered to be one of the most commonly used simple techniques. Drugs that lack chromogenic groups can be readily determined by using the chromogenic reagents, which react with the functional groups present in the drugs and produce a chromogenic group that can be detected in the visible region using a spectrophotometer. Chromogenic reagents play a vital role in the estimation of such types of drugs. Vanillin is one of the chromogenic reagents that possess a carbonyl group that reacts with the drugs that possess amine moiety and results in the formation of Schiff’s base, which is a yellow-colored compound that can be detected by spectrophotometry. The present review gives insights into the reaction conditions and applications of the drugs that are estimated by using vanillin as a chromogenic label.