A Comprehensive Review of the Mycelial Pellet: Research Status, Applications, and Future Prospects

Li, Lixin; Liang, Taojie; Liu, Wanmeng; Liu, Yan; Ma, Fang

doi:10.1021/acs.iecr.0c01325

Cited by 28 publications

(13 citation statements)

References 116 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nutrients in the fermentation medium have a significant impact on the formation, growth, and function of the mycelial pellet. Carbon sources and nitrogen sources are the most essential nutrients for mycelial pellets and product formation [47]. Concerning the use of carbon sources, the carbon sources of the various micellar pellets are different.…”

Section: Effect Of Nitrogen Sources On Enzyme Fermentationmentioning

confidence: 99%

Effect of process parameters and microparticle addition on polygalacturonase activity and fungal morphology of Aspergillus sojae

Germeç

Karahalil

Yatmaz

et al. 2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

In this study, the effects of process parameters (initial glucose concentrations, inoculation rates, and nitrogen sources including yeast extract, beef extract, and ammonium nitrate instead of peptone) on polygalacturonase production by Aspergillus sojae in the shaking incubator were examined. Subsequently, the effect of talcum microparticles on activity and morphology was investigated. Based on the results, the highest activity and lowest pellet diameter were 41.91 U/mL and 1411.9 µm when initial glucose concentration and inoculation size were 20 g/L and 12% (v/v), respectively. Besides, as inoculation rate increased, maximum specific growth rate and saturation constant decreased from 0.39 to 0.27 day −1 and 21.26 to 3.50 g/L, respectively. It was determined that highest activity and lowest pellet diameter were found as 18.53 U/mL and 2953.0 µm when medium was supplemented with 2.5 g/L yeast extract. It was also found that as yeast extract and beef extract concentrations increased, pellet diameter decreased. Additionally, medium was supplemented with talcum as a microparticle (0-25 g/L), and maximum activity was 26.59 U/mL (pellet diameter was 2756.3 µm) when talcum concentration was 5 g/L. In addition, as talcum concentration increases from 0.1 to 5 g/L, the biomass concentration increased relatively, but the enzyme activity increased significantly. However, although talcum concentration in the medium is increased until 20 g/L, while the biomass concentration increased, the activity decreased. Compared to control fermentation (without talcum), the activity increased ninefold. Polygalacturonase was also partially purified via ultrafiltration with the purification fold of 1.84. Consequently, fungal morphology in submerged fermentation can be controlled by microparticle addition fermentation, and thus the enzyme activity can be increased.

show abstract

Section: Effect Of Nitrogen Sources On Enzyme Fermentationmentioning

confidence: 99%

Effect of process parameters and microparticle addition on polygalacturonase activity and fungal morphology of Aspergillus sojae

Germeç

Karahalil

Yatmaz

et al. 2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

show abstract

“…Mycelial pellet formation or mycelial aggregation phenomenon is observed in some fungal species and influenced by certain cultivation parameter/conditions. Pellet forming fungi has applications in industrial fermentation, metal absorptions, dye absorption, micro‐algal biomass separation, as a biological carrier, and so forth (L. Li et al, 2020; Papagianni, 2004). In submerged fermentation, dispersed mycelial growth increases the broth viscosity which results in lower mass‐transfer or blocks the nutrient transport which consequently decreases its performance.…”

Section: Introductionmentioning

confidence: 99%

“…Another benefit of pellet form is that it can be easily separated and reused further. Energy consumption is one of the major cost factors which get reduced in case of pellet growth morphology fermentation (L. Li et al, 2020; Yu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Morphologically favorable mutant of Trichoderma reesei for low viscosity cellulase production

Adsul

Dixit

Saini

et al. 2022

Biotech & Bioengineering

View full text Add to dashboard Cite

Metabolite production by filamentous fungi hampered because of high viscosity generated during growth. Low viscosity fermentation by mold is one of the preferred ways of large scale enzymes production. Cellulolytic enzymes play a key role during the process of lignocellulosic biomass conversion. In this study, a mutant RC‐23‐1 was isolated through mutagenesis (diethyl sulfate followed by UV) of Trichoderma reesei RUT‐C30. RCRC‐23‐1 not only gave higher cellulase production but also generated lower viscosity during enzyme production. Viscosity of mutant growth was more than three times lower than parent strain. RC‐23‐1 shows unique, yeast‐like colony morphology on solid media and small pellet‐like growth in liquid media. This mutant did not spread like mold on solid media. This mutant produces cellulases constitutively when grown in sugars. Using only glucose, the cellulase production was 4.1 FPU/ml. Among polysaccharides (avicel, xylan, and pectin), avicel gave maximum of 6.2 FPU/ml and pretreated biomass (rice straw, wheat straw and sugarcane bagasse) produced 5.1–5.8 FPU/ml. At 7 L scale reactor, fed‐batch process was designed for cellulase production using different carbon and nitrogen sources. Maximum yield of cellulases was 182 FPU/g of lactose consumed was observed in fed‐batch process. The produced enzyme used for hydrolysis of acid pretreated rice straw (20% solid loading) and maximum of 60% glucan conversion was observed. RC‐23‐1 mutant is good candidate for large scale cellulase production and could be a model strain to study mold to yeast‐like transformation.

show abstract

“…However, the decentralized, intermittent, and constantly fluctuating energy supply from solar irradiation limits its inefficiency and scalability. Biomass energy, which originates from solar energy, has attracted great attention, because of the following aspects: − The sustainability of the biomass makes it an inexhaustible source worldwide. The biomass with a carbon component only from CO 2 in air results in a carbon-free emissions system. The diversity of biomass supply makes it possible to obtain different products through the transformation from bio-oil (gasoline, kerosene, and diesel) to various platform chemicals. There are almost no emissions of SO 2 , NO x , and soot, compared with conventional fossil fuels. …”

mentioning

confidence: 99%

“…To date, the conversion from the biomass to useful fuels and chemicals still remains a great challenge. As we know, the biomass sources are diverse and can mainly be classified into three categories: lignin, cellulose, and plant oils/animal fats. − Lignocellulosic biomass is the most abundant and least-expensive carbon source, which exists in wood, grass, and agricultural waste. The main compounds of the lignocellulosic biomass are complex and diverse both for compounds and chemical groups .…”

mentioning

confidence: 99%

The Future of Biomass Utilization Technologies Special Issue Editorial

Zhang

Ren

2020

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

show abstract

A Comprehensive Review of the Mycelial Pellet: Research Status, Applications, and Future Prospects

Cited by 28 publications

References 116 publications

Effect of process parameters and microparticle addition on polygalacturonase activity and fungal morphology of Aspergillus sojae

Effect of process parameters and microparticle addition on polygalacturonase activity and fungal morphology of Aspergillus sojae

Morphologically favorable mutant of Trichoderma reesei for low viscosity cellulase production

The Future of Biomass Utilization Technologies Special Issue Editorial

Contact Info

Product

Resources

About