A comprehensive review on carbon source effect of microalgae lipid accumulation for biofuel production

Ma, Xiangmeng; Mi, Yuwei; Zhao, Chen; Wei, Qun

doi:10.1016/j.scitotenv.2021.151387

Cited by 100 publications

(31 citation statements)

References 162 publications

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“…The TCA cycle, the main way to obtain energy for cell metabolism, is a common destination of the complete oxidation of starch, lipid and protein products [ 37 ]. Studies have shown that carbon sources can affect the energy supply due to the availability of carbon sources [ 47 ]. Glucose, as a direct carbon source, can provide more energy in energy metabolism in microalgae compared with sodium acetate [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Mechanisms of Sodium-Acetate-Induced DHA Accumulation in a DHA-Producing Microalga, Crypthecodinium sp. SUN

Tian

et al. 2022

Marine Drugs

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Docosahexaenoic acid (DHA) is an omega-3 polyunsaturated fatty acid (PUFA) that is critical for the intelligence and visual development of infants. Crypthecodinium is the first microalga approved by the Food and Drug Administration for DHA production, but its relatively high intracellular starch content restricts fatty acid accumulation. In this study, different carbon sources, including glucose (G), sodium acetate (S) and mixed carbon (M), were used to investigate the regulatory mechanisms of intracellular organic carbon distribution in Crypthecodinium sp. SUN. Results show that glucose favored cell growth and starch accumulation. Sodium acetate limited glucose utilization and starch accumulation but caused a significant increase in total fatty acid (TFA) accumulation and the DHA percentage. Thus, the DHA content in the S group was highest among three groups and reached a maximum (10.65% of DW) at 96 h that was 2.92-fold and 2.24-fold of that in the G and M groups, respectively. Comparative transcriptome analysis showed that rather than the expression of key genes in fatty acids biosynthesis, increased intracellular acetyl-CoA content appeared to be the key regulatory factor for TFA accumulation. Additionally, metabolome analysis showed that the accumulated DHA-rich metabolites of lipid biosynthesis might be the reason for the higher TFA content and DHA percentage of the S group. The present study provides valuable insights to guide further research in DHA production.

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Section: Resultsmentioning

confidence: 99%

Mechanisms of Sodium-Acetate-Induced DHA Accumulation in a DHA-Producing Microalga, Crypthecodinium sp. SUN

Tian

et al. 2022

Marine Drugs

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“…Among them, the YR-1 mutant showed the highest growth rate, which was about two times faster than the wild-type strain ( Figure 3B ). Microalgae are typically useful candidates for CO 2 fixation due to their rapid growth rate and high photosynthetic efficiency ( Ma et al, 2022 ). According to our results, the YR-1 mutant shows a growth rate similar to that of some microalgae, such as Chlorella vulgaris , Nannochloropsis oculata , under autotrophic conditions supplied with 10% of CO 2 ( Chiu et al, 2009 ; Lakshmikandan et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Regulation of Reactive Oxygen Species Promotes Growth and Carotenoid Production Under Autotrophic Conditions in Rhodobacter sphaeroides

Lee

et al. 2022

Front. Microbiol.

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Industrial demand for capture and utilization using microorganisms to reduce CO2, a major cause of global warming, is significantly increasing. Rhodobacter sphaeroides is a suitable strain for the process of converting CO2 into high-value materials because it can accept CO2 and has various metabolic pathways. However, it has been mainly studied for heterotrophic growth that uses sugars and organic acids as carbon sources, not autotrophic growth. Here, we report that the regulation of reactive oxygen species is critical for growth when using CO2 as a sole carbon source in R. sphaeroides. In general, the growth rate is much slower under autotrophic conditions compared to heterotrophic conditions. To improve this, we performed random mutagenesis using N-methyl-N’-nitro-N-nitrosoguanidine (NTG). As a result, we selected the YR-1 strain with a maximum specific growth rate (μ) 1.44 day–1 in the early growth phase, which has a 110% faster growth rate compared to the wild-type. Based on the transcriptome analysis, it was confirmed that the growth was more sensitive to reactive oxygen species under autotrophic conditions. In the YR-1 mutant, the endogenous contents of H2O2 levels and oxidative damage were reduced by 33.3 and 42.7% in the cells, respectively. Furthermore, we measured that concentrations of carotenoids, which are important antioxidants. The total carotenoid is produced 9.63 g/L in the YR-1 mutant, suggesting that the production is 1.7-fold higher than wild-type. Taken together, our observations indicate that controlling ROS promotes cell growth and carotenoid production under autotrophic conditions.

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“…The biological activity of microalgae is highly dependent on the culture conditions, and nutrients, carbon source, salinity, light, temperature, and metal ions all impact the lipid accumulation (Ma et al, 2022).…”

Section: Lipid Accumulationmentioning

confidence: 99%

A review on biodiesel production from microalgae: Influencing parameters and recent advanced technologies

Zhang

et al. 2022

Front. Microbiol.

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Microalgae are the important part of carbon cycle in the nature, and they could utilize the carbon resource in water and soil efficiently. The abilities of microalgae to mitigate CO2 emission and produce oil with a high productivity have been proven. Hence, this third-generation biodiesel should be popularized. This review firstly introduce the basic characteristics and application fields of microalgae. Then, the influencing parameters and recent advanced technologies for the microalgae biodiesel production have been discussed. In influencing parameters for biodiesel production section, the factors of microalgae cultivation, lipid accumulation, microalgae harvesting, and lipid extraction have been summarized. In recent advanced technologies for biodiesel production section, the microalgae cultivation systems, lipid induction technologies, microalgae harvesting technologies, and lipid extraction technologies have been reviewed. This review aims to provide useful information to help future development of efficient and commercially viable technology for microalgae-based biodiesel production.

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A comprehensive review on carbon source effect of microalgae lipid accumulation for biofuel production

Cited by 100 publications

References 162 publications

Mechanisms of Sodium-Acetate-Induced DHA Accumulation in a DHA-Producing Microalga, Crypthecodinium sp. SUN

Mechanisms of Sodium-Acetate-Induced DHA Accumulation in a DHA-Producing Microalga, Crypthecodinium sp. SUN

Regulation of Reactive Oxygen Species Promotes Growth and Carotenoid Production Under Autotrophic Conditions in Rhodobacter sphaeroides

A review on biodiesel production from microalgae: Influencing parameters and recent advanced technologies

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