The selection of tribopairs, including mating materials, plays a crucial role in controlling material loss under rubbing conditions. In a fast breeder nuclear reactor, almost all moving components have been provided with a hardfaced coating at the mating surfaces for their longer life. Though, in most cases, the hardfaced surfaces come into contact with the components having the same coating; in some instances, they are allowed to rub against other materials. In the present investigations, an effort was made to study the wear behaviour of a Ni-based hardfaced coating made on 316LN stainless steel under similar and dissimilar mating conditions. Prior to wear tests, a defect-free hardface coating of Ni-based hardfacing alloy was made on 316LN stainless steel using plasma transferred arc welding process and subsequently characterized for its microstructure and hardness. Wear tests under dry sliding conditions were carried out at ambient temperature. The specific wear rate for dissimilar mating conditions was found to be one order of magnitude higher than the same rate obtained from similar mating conditions. Moreover, adhesive wear was the dominant wear mechanism in both mating conditions. It is clear from the study that similar material combinations should be given preference in the design of tribopairs instead of dissimilar material combinations to avoid significantly higher material losses for materials with lower hardness. The reported results will provide insight for carefully designing the tribopairs for critical applications.