A comprehensive screening method for investigating the potential binding targets of doxorubicin based on protein microarray

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

“…In various studies, commercially available microarrays, such as the Explorer Antibody Microarray (ASB6000, Full Moon Biosystems, Sunnyvale, CA, USA) with 656 antibodies [ 95 ], VaxArray Coronavirus SeroAssay kit (#VXCV-5100, InDevR, Boulder, CO, USA) [ 96 ], Human Cytokine Antibody Microarray slides (AAH-CYT-G4000 kit, RayBiotech, Peachtree Corners, GA, USA) [ 97 ], Biotin-labeled Human antibody microarray (RayBio Biotech, Guangzhou, China) [ 98 ], Protein Microarray Slides (Grace Bio-Labs, Bend, OR, USA) [ 99 ], HuProt human proteome microarrays (CDI laboratories, Mayaguez, PR, USA) [ 100 ], SBC Mouse ceRNA microarray V1.0 (Shanghai Biotechnology Corporation, Shanghai, China) [ 101 ], and Agilent Whole Mouse 44 K ver. 2.0 microarray (Agilent technologies, Santa Clara, CA, USA) [ 102 ], were used.…”

“…This chemistry is used not only to immobilize the target biomolecule but also to conjugate fluorescence dye molecules. In the case of microarrays, several fluorescent dyes were conjugated with streptavidin (avidin-Cy3 [ 66 , 97 ], streptavidin-FITC [ 99 ], streptavidin–Alexa Fluor [ 68 , 98 ], streptavidin–fluorescent dye CF555 or CF647 [ 78 , 79 ], and streptavidin-Cy5 [ 100 ] conjugates) and are used to detect the biotin-labeled biomolecules present on the surface of a substrate.…”

“…A HuProt human protein microarray [ 100 ], in combination with a bioinformatics analysis, was used to screen and identify potential doxorubicin (Dox; an anti-tumor drug)-binding protein targets. A total of 27 proteins were shortlisted, and studies on one of the proteins, HRAS (Harvey rat sarcoma viral oncogene homolog), revealed that Dox promotes HRAS-RAF complex formation.…”