A comprehensive study of glucose transfer in the human small intestine using an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane process

Gim-Krumm, Minghai; Donoso, Pablo J.; Zúñiga, Rommy N.; Estay, Humberto; Troncoso, Elizabeth

doi:10.1016/j.memsci.2018.07.080

Cited by 9 publications

(30 citation statements)

References 44 publications

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“…Therefore, in recent years, there has been a development of dynamic in vitro models capable of simulating the physiology of the stomach and small intestine, which can predict in vivo behavior, such as decomposition of solids and nutrient absorption. These models can be used as a valuable research tool for the study and understanding of changes and interactions as well as the bioaccessibility and bioavailability of nutrients, drugs and non-nutrient compounds [ 5 , 6 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…To simulate in vitro absorption, artificial or biological membrane systems or assays based on biological cell monolayers have been used [ 24 , 25 , 26 ]. For in vitro intestinal systems, semipermeable membranes are often used, which act as a selective barrier to keep the enzymes separated from their digestion products, avoiding the inhibition of enzymatic hydrolysis [ 18 ]. Recently, Dupont et al [ 5 ] reviewed the validation of eight dynamic in vitro models against in vivo data, namely DGM, HGS, ARCOL, DIDGI ® , TIM, SHIME ® , ESIN and simgi ® .…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, the TIM and ESIN models use a dialysis hollow fiber membrane to mimic the passive absorption of water and hydrolytic products from digestion in the small intestine. A description of other more established models based on membrane systems can be found in Gim et al [ 18 ], where the advantages and limitations of existing approaches are briefly discussed. For example, in 2010, the in vitro small intestine model (SIM) [ 27 ] was fabricated to study glucose absorption using semi-permeable dialysis membranes, also mimicking intestinal peristalsis and segmentation motion; however, fluids secretion, pH and body temperature were not considered.…”

Section: Introductionmentioning

confidence: 99%

“…The dialysis process is the only membrane process capable of transferring solutes with high molecular weight by diffusive mass transfer, due to a concentration difference. Considering this last point, in a previous work performed by our research team [ 18 ], we fabricated an in vitro small intestine model ( i -IDS) where a dialysis membrane system was used as an artificial small intestine, due to its ability to simulate the phenomena of diffusive mass transfer and absorption occurring in the small intestine and the arrangement of the large transfer area of the membrane module. The system allowed glucose transfer and absorption to be studied.…”

Section: Introductionmentioning

confidence: 99%

“…Based on the above, the objective of this work is to deepen our previous study [ 18 ], using the same in vitro digestion system, namely the i -IDS ( Figure 1 ). In this system, a dialysis membrane was used to simulate the absorption process of glucose molecules in the small intestine.…”

Section: Introductionmentioning

confidence: 99%

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Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process

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This work deepens our understanding of starch digestion and the consequent absorption of hydrolytic products generated in the human small intestine. Gelatinized starch dispersions were digested with α-amylase in an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane process. This study innovates with respect to the existing literature, because it considers the impact of simultaneous digestion and absorption processes occurring during the intestinal digestion of starchy foods and adopts phenomenological models that deal in a more realistic manner with the behavior found in the small intestine. Operating the i-IDS at different flow/dialysate flow ratios resulted in distinct generation and transfer curves of reducing sugars mass. This indicates that the operating conditions affected the mass transfer by diffusion and convection. However, the transfer process was also affected by membrane fouling, a dynamic phenomenon that occurred in the i-IDS. The experimental results were extrapolated to the human small intestine, where the times reached to transfer the hydrolytic products ranged between 30 and 64 min, according to the flow ratio used. We consider that the i-IDS is a versatile system that can be used for assessing and/or comparing digestion and absorption behaviors of different starch-based food matrices as found in the human small intestine, but the formation and interpretation of membrane fouling requires further studies for a better understanding at physiological level. In addition, further studies with the i-IDS are required if food matrices based on fat, proteins or more complex carbohydrates are of interest for testing. Moreover, a next improvement step of the i-IDS must include the simulation of some physiological events (e.g., electrolytes addition, enzyme activities, bile, dilution and pH) occurring in the human small intestine, in order to improve the comparison with in vivo data.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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This study aims to design a smart closed reactor of vermicomposting to convert sewage sludge and any organic waste to high-quality vermicompost. In this reactor design, all aspects of growth and reproduction of Eisenia Fetida worms, such as aeration, temperature, light, and moisture, were considered. We analyzed the physicochemical, bacterial, and microstructural of produced vermicompost and growth rate of worms in a substrate of 70% sewage sludge, 20% cow manure, and 10% sugarcane bagasse in a container and the smart reactor. The results show that vermicomposting in the smart reactor took 50% less time and 30% more worm growth rate to produce the same quality as in a container. After vermicomposting in the reactor, the parameters of pH, fecal coliform, phosphorus, organic matter, and C/N decreased whereas the parameters of carbon, nitrogen, nitrate, ammonia nitrate, and EC increased, slightly. Although, the EC amount of the reactor production is more than the container one, the amount of moisture, phosphorus, and organic matter of the vermicompost in the container is more than the reactor one.Based on the odor absorption and leachate elimination of this reactor, we recommend that it be utilized for vermicompost production, including out of smelly organic wastes such as sewage sludge, even in any public zone and personal houses.

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A dialysis membrane process for the in vitro simulation of bile acids absorption in the small intestine

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A comprehensive study of glucose transfer in the human small intestine using an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane process

Cited by 9 publications

References 44 publications

Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process

Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process

Vermicomposting Smart Closed Reactor Design and Performance Assessment by Using Sewage Sludge

A dialysis membrane process for the in vitro simulation of bile acids absorption in the small intestine

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