A comprehensive study of lipase production by<i>Yarrowia lipolytica</i>CECT 1240 (ATCC 18942): from shake flask to continuous bioreactor

Deive, Francisco J.; Sanromán, M.A.; Longo, María A.

doi:10.1002/jctb.2301

Cited by 30 publications

(25 citation statements)

References 44 publications

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“…Generally, the serine residue occurs in the pentapeptide chain of Gly-Xaa-Ser-Xaa-Gly, except in the Bacillus lipases where the first glycine is replaced by an alanine (Tyndall et al 2002). Enhancing the lipase production during the cultivation process is an important step in industrial application of lipases (Silva et al 2005;Deive et al 2010). Different environmental factors affecting lipase production, such as carbon sources, inducer type and concentration, pH, and temperature for various microbial sources have been extensively studied to increase lipase productivity (Sztajer et al 1988;Cruz et al 1993;Hou 1994;Shidhu et al 1998;Abdel-Fattah 2002;Aravindan and Viruthagiri 2007;Griebeler et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Optimization and Modeling of Process Parameters for Lipase Production by Bacillus brevis

Aravindan

Viruthagiri

2010

Food Bioprocess Technol

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Statistical evaluation of fermentation conditions and nutritional factors by Plackett-Burman two-level factorial design followed by optimization of significant parameters using response surface methodology for lipase production by Bacillus brevis was performed in submerged batch fermentation. Temperature, glucose, and olive oil were found to be the significant factors affecting lipase production. Maximum lipase activity of 5.

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Section: Introductionmentioning

confidence: 99%

Optimization and Modeling of Process Parameters for Lipase Production by Bacillus brevis

Aravindan

Viruthagiri

2010

Food Bioprocess Technol

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“…In order to gain a deeper knowledge of the characteristics of the enzyme produced, a study of enzyme distribution was performed. As reported by Deive et al , this type of study is an important step in determining adequate downstream strategies to purify the enzymes obtained. From the data depicted in Fig.…”

Section: Resultsmentioning

confidence: 95%

“…The search for more efficient bioprocesses at an industrial scale has prompted the investigation of a number of small‐scale bioreactor systems, which tended to focus on stirred tank or airlift designs because of their geometrical similarity with the large‐scale bioreactors most likely to be used for industrial manufacture . Besides, these configurations allow evaluating the importance of different decisive engineering variables such as agitation and aeration on the production of chitinolytic enzymes.…”

Section: Resultsmentioning

confidence: 99%

Efficient biosynthesis of a chitinase from Halobacterium salinarum expressed in Escherichia coli

Moscoso

Sieira

Domínguez

et al. 2013

J of Chemical Tech & Biotech

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BACKGROUND Chitinases have been widely studied and can be produced by various groups of microorganisms, in particular, marine bacteria. The exploration of extremophilic marine microorganisms bears advantageous and unique biotechnological implications and the scaling up of the production process is a decisive stage to be researched. RESULTS A chitinase gene from Halobacterium salinarum was expressed in Escherichia coli and the protein production process was performed at different scales. In order to determine the most suitable bioreactor, two configurations were investigated: stirred‐tank and airlift. It was demonstrated that the stirred‐tank configuration led to the highest chitinase activities. The effect of stirrer speed (range 150–400 rpm) and aeration rate (range 0.6–2 vvm) was investigated, and maximum activity levels of 20.99 U L‐1 were detected at 300 rpm and 0.66 vvm CONCLUSION Chitinase from H. salinarum expressed in E. coli was successfully produced in a mechanically stirred bioreactor, and the optimum conditions of agitation and aeration were ascertained. The chitinase obtained turned out to possess a mass weight of 66 kDa, as determined by SDS‐PAGE. The system was suitably characterized with known mathematical models. © 2013 Society of Chemical Industry

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“…as well (Makri et al, 2010;Economou et al, 2011;Tsigie et al, 2013). Y. lipolytica has been cultivated in various conditions and bioreactors types (stirred or gas-lift bioreactors, using the batch, fed-batch or continuous regime), but the engineering aspects useful for scaling-up, namely effects of operating conditions on broths hydrodynamics and cells mechanical integrity, rates of mass and heat transfers, etc., have not been completely elucidated (Deive et al, 2010). Among the three main types of pneumatic bioreactors, namely bubble columns, gas-lift, and with fluidization ones, the gas-lift bioreactors are the most attractive especially for industrial-scale applications.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Mixing Efficiency in a Split-Cylinder Gas-Lift Bioreactor with ImmobilizedYarrowia LipolyticaCells Used for Olive Oil Mill Wastewater Treatment

Matran

Galaction

Blaga

et al. 2015

Chemical Engineering Communications

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The distribution of mixing in a split-cylinder gas-lift bioreactor has been investigated for suspensions of immobilized Y. lipolytica cells with particles diameter varying between 3 and 4.2 mm. The results indicated important variation of mixing time on the height of riser or downcomer, as well as different behavior of suspensions flows in these two regions. Therefore, for the riser, the mixing efficiency increases from its bottom to the top, the biocatalyst particles with intermediary size (3.6 mm diameter) allowing reaching the most intense circulation of suspension. The analysis of the suspension flow in the downcomer region revealed that the intermediary positions are associated with the highest mixing mainly for the largest immobilized yeast particles (4.2 mm diameter). In both cases, the influence of aeration on turbulence extent is positive only for air superficial velocity up to 1.05 -1.35x10 -3 m/s, the magnitude of this effect being correlated with the biocatalyst size and position on the riser or downcomer. By means of the experimental data, mathematical correlations for mixing time have been proposed for each circulation region, taking into consideration both the operational parameters and the Downloaded by [University of Otago] at 10:37 05 October 2015 2 distance from the bioreactor bottom. These equations offer a good concordance with the experiment, the average deviation being of 5.82 for the riser and 6.06% for the downcomer zone.

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A comprehensive study of lipase production byYarrowia lipolyticaCECT 1240 (ATCC 18942): from shake flask to continuous bioreactor

Cited by 30 publications

References 44 publications

Optimization and Modeling of Process Parameters for Lipase Production by Bacillus brevis

Optimization and Modeling of Process Parameters for Lipase Production by Bacillus brevis

Efficient biosynthesis of a chitinase from Halobacterium salinarum expressed in Escherichia coli

Distribution of Mixing Efficiency in a Split-Cylinder Gas-Lift Bioreactor with ImmobilizedYarrowia LipolyticaCells Used for Olive Oil Mill Wastewater Treatment

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