A Comprehensive Study of Molecular Evolution at the Self-Incompatibility Locus of Rosaceae

Ashkani, Jahanshah; Rees, D. J. G.

doi:10.1007/s00239-015-9726-4

Cited by 21 publications

(18 citation statements)

References 111 publications

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“…In agreement with what was previously reported 36 , these models consist of α-helices and β-strands that are inter-connected by loops. The variation that we observed in the numbers, lengths and positions of secondary structure elements was similar to that reported for S-RNase proteins of Japanese pear and apple 19,37,38 but greater than what had been reported for almond 36 . This difference is likely due to the more comprehensive modelling processes used here, with several tools used to align protein sequences and identify mismatches, many models generated from slightly different alignments and final models selected based on optimisation and evaluation of binding energy for multiple stable low-energy models.…”

Section: Discussionsupporting

confidence: 86%

Variation among S-locus haplotypes and among stylar RNases in almond

Goonetilleke

Croxford

March

et al. 2020

Sci Rep

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in many plant species, self-incompatibility systems limit self-pollination and mating among relatives. this helps maintain genetic diversity in natural populations but imposes constraints in agriculture and plant breeding. in almond [Prunus dulcis (Mill.) D.A. Webb], the specificity of self-incompatibility is mainly determined by stylar ribonuclease (S-Rnase) and S-haplotype-specific F-box (SFB) proteins, both encoded within a complex locus, S. prior to this research, a nearly complete sequence was available for one S-locus haplotype. Here, we report complete sequences for four haplotypes and partial sequences for 11 haplotypes. Haplotypes vary in sequences of genes (particularly S-RNase and SFB), distances between genes and numbers and positions of long terminal repeat transposons. Haplotype variation outside of the S-RNase and SFB genes may help maintain functionally important associations between S-RNase and SFB alleles. fluorescence-based assays were developed to distinguish among some S-RNase alleles. With three-dimensional modelling of five S-RNase proteins, conserved active sites were identified and variation was observed in electrostatic potential and in the numbers, characteristics and positions of secondary structural elements, loop anchoring points and glycosylation sites. A hypervariable region on the protein surface and differences in the number, location and types of glycosylation sites may contribute to determining S-RNase specificity.

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Section: Discussionsupporting

confidence: 86%

Variation among S-locus haplotypes and among stylar RNases in almond

Goonetilleke

Croxford

March

et al. 2020

Sci Rep

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“…As described for other Rosaceae species [40–42], in some diploid roses self-incompatibility (SI) is caused by a gametophytic SI-locus. This locus is most probably composed of genes encoding S-RNAses and F-Box proteins, which represent the female and male specific components, respectively.…”

Section: Resultsmentioning

confidence: 99%

A high-quality sequence ofRosa chinensisto elucidate genome structure and ornamental traits

Hibrand

Ruttink

Hamama

et al. 2018

Preprint

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Rose is the world’s most important ornamental plant with economic, cultural and symbolic value. Roses are cultivated worldwide and sold as garden roses, cut flowers and potted plants. Rose has a complex genome with high heterozygosity and various ploidy levels. Our objectives were (i) to develop the first high-quality reference genome sequence for the genus Rosa by sequencing a doubled haploid, combining long and short read sequencing, and anchoring to a high-density genetic map and (ii) to study the genome structure and the genetic basis of major ornamental traits.We produced a haploid rose line from R. chinensis ‘Old Blush’ and generated the first rose genome sequence at the pseudo-molecule scale (512 Mbp with N50 of 3.4 Mb and L75 of 97). The sequence was validated using high-density diploid and tetraploid genetic maps. We delineated hallmark chromosomal features including the pericentromeric regions through annotation of TE families and positioned centromeric repeats using FISH. Genetic diversity was analysed by resequencing eight Rosa species. Combining genetic and genomic approaches, we identified potential genetic regulators of key ornamental traits, including prickle density and number of flower petals. A rose APETALA2 homologue is proposed to be the major regulator of petals number in rose. This reference sequence is an important resource for studying polyploidisation, meiosis and developmental processes as we demonstrated for flower and prickle development. This reference sequence will also accelerate breeding through the development of molecular markers linked to traits, the identification of the genes underlying them and the exploitation of synteny across Rosaceae.

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“…However, despite the fact that several models have been developed to explain how the two components of the S-locus interact, the mechanism by which SLF/SFB ( S-haplotype-specific F-box ) and S-RNase interact to trigger the SI reaction remains uncertain (Akagi et al, 2016; Ashkani and Rees, 2016). Moreover, the speculative GSI models based on SLF are often paradoxical.…”

Section: Introductionmentioning

confidence: 99%

PLC-Mediated Signaling Pathway in Pollen Tubes Regulates the Gametophytic Self-incompatibility of Pyrus Species

Guan

Wang

et al. 2017

Front. Plant Sci.

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Among the Rosaceae species, the gametophytic self-incompatibility (GSI) is controlled by a single multi-allelic S locus, which is composed of the pistil-S and pollen-S genes. The pistil-S gene encodes a polymorphic ribonuclease (S-RNase), which is essential for identifying self-pollen. However, the S-RNase system has not been fully characterized. In this study, the self-S-RNase inhibited the Ca2+-permeable channel activity at pollen tube apices and the selectively decreased phospholipase C (PLC) activity in the plasma membrane of Pyrus pyrifolia pollen tubes. Self-S-RNase decreased the Ca2+ influx through a PLC-mediated signaling pathway. Phosphatidylinositol-specific PLC has a 26-amino acid insertion in pollen tubes of the ‘Jinzhuili’ cultivar, which is a spontaneous self-compatible mutant of the ‘Yali’ cultivar. ‘Yali’ plants exhibit a typical S-RNase-based GSI. Upon self-pollination, PLC gene expression is significantly higher in ‘Jinzhuili’ pollen tubes than that in ‘Yali’ pollen tubes. Moreover, the PLC in pollen tubes can only interact with one of the two types of S-RNase from the style. In the Pyrus x bretschneideri Rehd, the PLC directly interacted with the S7-RNase in the pollen tube, but not with the S34-RNase. Collectively, our results reveal that the effects of S-RNase on PLC activity are required for S-specific pollen rejection, and that PLC-IP3 participates in the self-incompatibility reaction of Pyrus species.

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A Comprehensive Study of Molecular Evolution at the Self-Incompatibility Locus of Rosaceae

Cited by 21 publications

References 111 publications

Variation among S-locus haplotypes and among stylar RNases in almond

Variation among S-locus haplotypes and among stylar RNases in almond

A high-quality sequence ofRosa chinensisto elucidate genome structure and ornamental traits

PLC-Mediated Signaling Pathway in Pollen Tubes Regulates the Gametophytic Self-incompatibility of Pyrus Species

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