A comprehensive study on bisphenol A degradation by newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12

Abstract

Bisphenol A (BPA) is an endocrine disrupting chemical. Its extensive use has led to the wide occurrence of BPA in various environmental ecosystems, at levels that may cause negative effects to the ecosystem and public health. Although there are many bacteria able to BPA utilization, only a few of them have a strong capacity for its biodegradation. Therefore, it is important to search for new bacteria strains, investigate their BPA biodegradation ability and potential effect of pH and other organic compounds on the process. These tasks have become the object of the present study. The results of our research show that for the newly isolated strains Acinetobacter sp. K1MN and Pseudomonas sp. BG12 after 15 days, with an initial BPA concentration of 100 mg L− 1, the highest BPA removal was achieved at pH 8, while sodium glutamate as a biostimulant best accelerated BPA degradation. Kinetic data for BPA biodegradation by both strains best fitted the Monod model. The specific degradation rate and the half saturation constant were estimated respectively as 8.75 mg L− 1 day− 1 and 111.27 mg L− 1 for Acinetobacter sp. K1MN, and 8.6 mg L− 1 day− 1 and 135.79 mg L− 1 for Pseudomonas sp. BG12. The half-maximal effective concentration (EC50) of BPA for Acinetobacter sp. K1MN was 120 mg L− 1 and for Pseudomonas sp. BG12 it was 123 mg L− 1. The toxicity bioassay (Microtox test) showed that elimination of BPA by both strains is accompanied by reduction of its toxic effect. The ability of tested strains to degrade BPA combined with their high resistance to this xenobiotic indicates that Acinetobacter sp. K1MN and Pseudomonas sp. BG12 are potential tools for BPA removal during wastewater treatment plant.

Bioremediation of a salty petrochemical wastewater containing bisphenol A by a novel indigenous Pseudomonas pseudoalcaligenes

“…29,37 Up to now, many bacteria with BPA-biodegradation activity have been isolated from different environments, but only a few of them have a strong capacity for its biodegradation. [3][4][5]8,9,11,[16][17][18][19][20][21][22]24,[26][27][28][29]31 However, strains that can quickly and completely degrade and utilize BPA as sole carbon source without accumulation of intermediate compounds in the medium are more desirable. Pseudomonas sp.…”

“…3,5,9,12–15 Therefore, providing an efficient eco-friendly solution for its removal from exposed natural environments is so necessary. According to the results of several studies 3,5,6,8,9,11,16–24 as well as our previous research, 25 bioremediation using BPA-degrading bacteria has been proved to be an effective way to remove this compound. Up to now, many BPA-degrading bacteria have been isolated from soil, sediment, water and petrochemical wastewater and BPA-biodegradation pathways have been recognized in most of them.…”

“…Up to now, many BPA-degrading bacteria have been isolated from soil, sediment, water and petrochemical wastewater and BPA-biodegradation pathways have been recognized in most of them. [3][4][5][6][7][8][9]11,[16][17][18][19][20][21][22][23][24][25][26][27][28][29] Lobos et al 19 and Spivack et al 30 were the rst to describe two major and minor pathways for BPAbiodegradation by Sphingomonas sp. strain MV1.…”

Lysinibacillus sp. GG242 from Cattle Slurries Degrades 17β-Estradiol and Possible 2 Transformation Routes

“…pH, temperature, and salinity are frequently mentioned influencing factors. Strong alkali or acid affect the activity of microorganisms [32,33], and the degradation rate of strain GG242 also decreases at high or low pH. Therefore, appropriate pH is one of the keys for bacteria to function, in addition to affecting microorganisms, pH affects the removal of TCP (2,4,6trichlorophenol) by ferrate [34].…”

A Chemical Invasion on Waters and Aquatic Organisms: Bisphenol A

“…(Kolvenbach et al, 2007;Oshiman et al, 2007;Sakai et al, 2007;Yamanaka et al, 2008;Matsumura et al, 2009;Jia et al, 2020;Gao et al, 2022) Pseudomonas sp. (Kang & Kondo, 2002;Zhang et al, 2007;Fischer et al, 2010;Him et al, 2017;Kučić Grgić et al, 2019;Eltoukhy et al, 2020;Noszczyńska et al, 2021) Achromobacter sp (Zhang et al, 2007;Fischer et al, Streptomyces sp. (Kang vd., 2004;Matsumura et al, 2009;Saiyood et al, 2010;Kamaraj et al, 2014;Das et al, 2018;Kučić Grgić et al, 2019) Bacillus sp.…”