A comprehensively prognostic and immunological analysis of chloride intracellular channel protein 5 (CLIC5) in pan-cancer and identification in ovarian cancer

Huang, Qiaoling; Lv, Q.; Tang, Waner; Pan, Yunfeng; Xing, Yue; He, Min; Wu, Han; Huang, Junpeng; Che, Huang; Lan, Haifeng; Chen, Jingqi; Xiao, Guohong

doi:10.1007/s00432-023-04927-4

Cited by 3 publications

(4 citation statements)

References 44 publications

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“…Chloride channel, CLIC1, CLIC4 are excessively expressed in PAH and contribute to mitochondrial dysfunction and energy metabolism in endothelium [33,34]. CLIC5 encodes actin-based cytoskeletal protein, and has been thought to play signi cant roles in human cancers [35]. Enrichment result suggested that CLIC5 might participate in obsolete peroxidase reaction and was associated with PAH.…”

Section: Discussionmentioning

confidence: 99%

Integration of transcriptomics, proteomics, phosphoproteomics analysis for characterization of pulmonary arterial hypertension in Chinese people

liu,

Zhou,

Wang

et al. 2024

Preprint

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Background Pulmonary arterial hypertension (PAH), a fatal disease, is characterized by pulmonary vascular remodeling and vascular resistance. However, the molecular mechanisms underlying the pathogenesis of PAH remained to be incompletely understood. Methods RNA-seq, 4D Lable-free proteomics and phosphoproteomics were used to detect the levels of mRNA, proteins, and phosphoproteins in lung tissues from PAH patients, respectively. Parallel reaction monitoring (PRM) was carried out to verify the expression of the differentially expressed proteins. Results Totally, 967 differentially expressed genes (|log2FoldChange|>1 and p < 0.05), 764 differentially expressed proteins and 411 phosphoproteins were observed after data filtering (|log2FoldChange|>1 and p < 0.05) in lung tissues of PAH patients as compared with the control group. Integrated analysis of the three omic measures revealed that the biological processes involving inflammation, ion channel and metabolism were closely associated with PAH. Several signaling pathways, such as ferroptosis, HIF-1, PI3K-AKT, and Rap1 might be related to the development of PAH. Conclusions This study combined multi-omics characteristic profiling to find out the changed genes or proteins that contributed to a detailed pathogenic of PAH. It would have the benefit of looking for the novel and effective treatment targets and therapeutic drugs to PAH patients.

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Section: Discussionmentioning

confidence: 99%

Integration of transcriptomics, proteomics, phosphoproteomics analysis for characterization of pulmonary arterial hypertension in Chinese people

liu,

Zhou,

Wang

et al. 2024

Preprint

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“…Authors suggested that CLIC5 might be a scaffold for EZR and PODXL proteins, and this complex collectively plays a role in invasion and migration of HCC cells. High CLIC5 protein expression was correlated with increased infiltration of CD163+ M2 macrophages and decreased infiltration of CD8+ T cells in the ovarian cancer TME [10]. Finally, Neveu et al reported that CLIC5 could be the ETV6 target gene in childhood ALL and hypothesized that CLIC5A overexpression generates a permissive environment for consecutive mutations leading to leukemic transformation [64].…”

Section: Knockdown Of Clic1 In Escc Tissues Inhibited Cells' Prolifer...mentioning

confidence: 98%

“…On the other hand, Peng et al reported CLIC4 protein overexpression in bloodsecreted exosomes and ovarian cancer tissues, proposing CLIC4 protein as a potential epithelial ovarian carcinoma blood biomarker [18]. Different patterns of CLIC5 expression were found in childhood acute lymphoblastic leukemia (ALL), lung adenocarcinoma, HCC, and ovarian cancer [10,60,61,64]. Bian et al reported decreased CLIC5 gene expression in lung adenocarcinoma tissues, which was associated with poor overall survival [60].…”

Section: Knockdown Of Clic1 In Escc Tissues Inhibited Cells' Prolifer...mentioning

confidence: 99%

“…CLICs expression is deregulated in various types of cancers, as they are involved in carcinogenetic processes on the molecular level [9]. Several papers reported a significant role of CLICs in the TME, including correlation with immune cells infiltration, taking part in progression and metastasis, and CLIC1 secretion into interstitial fluid (10)(11)(12)(13). Cancer cells secrete CLIC proteins into blood, enabling a potentially feasible approach to monitor their level by the conception of liquid biopsy [11,[14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Chloride intracellular channels in oncology as potential novel biomarkers and personalized therapy targets: a systematic review

Wojtera,

Ostrowska,

Szewczyk

et al. 2024

Rep Pract Oncol Radiother.

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Dachsous cadherin related 1 (DCHS1) is a novel biomarker for immune infiltration and epithelial-mesenchymal transition in endometrial cancer via pan-cancer analysis

Meijuan,

Fang,

qian

2024

J Ovarian Res

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Background Dachsous cadherin related 1 (DCHS1) is one of calcium-dependent adhesion membrane proteins and is mainly involved in the development of mammalian tissues. There is a lack of more detailed research on the biological function of DCHS1 in pan-cancer. Materials and methods We evaluated the expression, the prognostic value, the diagnostic value and genomic alterations of DCHS1 by using the databases, including TCGA, UALCAN, HPA, GEPIA2.0 and GSCA. We employed the databases of UCSC, TIMER2.0, TISIDB, GSCA to analyze the association between DCHS1 expression and the immune microenvironment, stemness, TMB, MSI and anticancer drug sensitivity. BioGRID, STRING and GEPIA2.0 were used to perform protein interaction and functional enrichment analysis. Real-time quantitative PCR, CCK8, Transwell assay and Western blot were performed to determine the function of DCHS1 in UCEC. Results DCHS1 is differentially expressed in many cancers and its expression is significantly associated with tumor prognosis and diagnosis. DCHS1 expression was significantly correlated with the infiltration of cancer-associated fibroblasts (CAFs), Endothelial cell (ECs), and Hematopoietic stem cell in most cancers. In addition, DCHS1 was significantly associated with sensitivity to many antitumor drugs. Functional enrichment analysis revealed that DCHS1-related proteins were involved in Focal adhesion, Endometrial cancer and Wnt signaling pathway. GSEA results showed that DCHS1 was related to epithelial-mesenchymal transition (EMT) in many cancers. In vitro experiments in UCEC showed that DCHS1 regulated cell proliferation, migration and EMT. Conclusions Our findings indicated that DCHS1 might be a novel prognostic and diagnostic biomarker and immunotherapy target, and plays an important role in the proliferation, migration and EMT in UCEC.

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A comprehensively prognostic and immunological analysis of chloride intracellular channel protein 5 (CLIC5) in pan-cancer and identification in ovarian cancer

Cited by 3 publications

References 44 publications

Integration of transcriptomics, proteomics, phosphoproteomics analysis for characterization of pulmonary arterial hypertension in Chinese people

Integration of transcriptomics, proteomics, phosphoproteomics analysis for characterization of pulmonary arterial hypertension in Chinese people

Chloride intracellular channels in oncology as potential novel biomarkers and personalized therapy targets: a systematic review

Dachsous cadherin related 1 (DCHS1) is a novel biomarker for immune infiltration and epithelial-mesenchymal transition in endometrial cancer via pan-cancer analysis

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