Ozone (O3) pollution is a serious issue in China, posing a significant threat to people’s health. Traffic emissions are the main pollutant source in urban areas. NOX and volatile organic compounds (VOCs) from traffic emissions are the main precursors of O3. Thus, it is crucial to investigate the relationship between traffic conditions and O3 pollution. This study focused on the potential relationship between O3 concentration and traffic conditions at a roadside and urban background in Guangzhou, one of the largest cities in China. The results demonstrated that no significant difference in the O3 concentration was observed between roadside and urban background environments. However, the O3 concentration was 2 to 3 times higher on sunny days (above 90 μg/m3) than on cloudy days due to meteorological conditions. The results confirmed that limiting traffic emissions may increase O3 concentrations in Guangzhou. Therefore, the focus should be on industrial, energy, and transportation emission mitigation and the influence of meteorological conditions to minimize O3 pollution. The results in this study provide some theoretical basis for mitigation emission policies in China.