A computational study of the interaction between dopamine and DNA/RNA nucleosides

Skúpa, Katarína; Melicherčík, Milan; Urban, Ján

doi:10.1007/s00894-015-2788-9

Cited by 5 publications

(2 citation statements)

References 56 publications

(65 reference statements)

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“…36 Many physical, chemical, and computational studies have reported Dopamine can bind to a double helical linear DNA molecule. [37][38][39] Therefore, based on the above literature reports, the properties of DNA tetrahedral nanocages as blood-brain barrier crossing nanoparticles 15,34 and their excellent drug delivery efficacy 23 and neurotherapeutic efficiencies 15,35 , we hypothesize that DNA tetrahedron can be a viable delivery system for effective delivery of Dopamine. The Dopamine-loaded DNA tetrahedron can be potential therapeutics in treating Parkinson's disease.…”

Section: Introductionmentioning

confidence: 97%

DNA Tetrahedral Nanocages as a Promising Nanocarrier for Dopamine Delivery in Neurological Disorders

Singh,

Kansara,

Mandal

et al. 2023

Preprint

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Dopamine is a neurotransmitter in the central nervous system that is essential for many bodily and mental processes, and a lack of it can cause Parkinson’s disease. DNA tetrahedral (TD) nanocages are promising in bio-nanotechnology, especially as a nanocarrier. TD is highly programmable, biocompatible, and capable of cell differentiation and proliferation. It also has tissue and blood-brain barrier permeability, making it a powerful tool that could overcome potential barriers in treating neurological disorders. In this study, we used DNA-TD as a carrier for Dopamine in cells and zebrafish embryos. We investigated the mechanism of complexation between TD and dopamine hydrochloride using gel electrophoresis, fluorescence and circular dichroism (CD) spectroscopy, atomic force microscopy (AFM), and molecular dynamic (MD) simulation tools. Further, we demonstrate these Dopamine-loaded DNA tetrahedral nanostructures’ cellular uptake and differentiation ability in SH-SY5Y neuroblastoma cells. Furthermore, we extended the study to zebrafish embryos as a model organism to examine survival and uptake. The research provides valuable insights into the complexation mechanism and cellular uptake of dopamine-loaded DNA tetrahedral nanostructures, paving the way for further advancements in nanomedicine for Parkinson’s disease and other neurological disorders.

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Section: Introductionmentioning

confidence: 97%

DNA Tetrahedral Nanocages as a Promising Nanocarrier for Dopamine Delivery in Neurological Disorders

Singh,

Kansara,

Mandal

et al. 2023

Preprint

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“…Based on this principle, we designed a cationic polycatechol, poly( N , N′ -bis(acryloyl)cystamine- co -dopamine) (PBD), featuring catechol groups as the side chain. Catechol groups can bind mRNA nucleobases by hydrogen bonding interactions. ,, The dual interactions (electrostatic interaction and hydrogen bonding interaction) allow the polymer to form much denser and hydrophobic particles with mRNA against hydrolysis to reach excellent storage stability ( Q S ) (Scheme ). The disulfide bond on the main chain enables the polymer to degrade in response to glutathione (GSH) in cells and then release mRNA ( Q R ).…”

Section: Introductionmentioning

confidence: 99%

Rational Design of Polymeric mRNA Delivery Vectors to Achieve Excellent Room-Temperature Storage Stability and Delivery Efficiency

Shi,

Yin,

Sun

et al. 2024

Chem. Mater.

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Delivery vectors play an important role in delivering mRNA. However, it is an important challenge to enhance roomtemperature storage stability and balance the conflicting requirements for excellent in vivo stability and mRNA release performance. In response to the above problems, we propose a "4Q" principle for designing mRNA delivery vectors. The overall delivery efficiency (Q) of mRNA is related to the stability of the delivery vector itself (Q S , including storage stability and in vivo stability which was rarely considered previously), the performance of diffusion to the target cells (Q D ), the ability to enter the cytoplasm through the cell membrane (Q I ), and the performance of the intracellular release of mRNA (Q R ). The efficiencies of each step are crucial to high overall delivery efficiency of intramuscular injection. Based on this "4Q" principle, we designed a new cationic polycatechol, poly(N,N′-bis(acryloyl)cystamine-co-dopamine) (PBD). The PBD/mRNA polyplexes are stable at room temperature for more than 2 weeks (Q S ↑). The in vivo transfection fluorescence intensity of polyplexes was 2 orders of magnitude higher than the commercially available mRNA delivery system (e.g., jetPEI/mRNA). Overall, this new "4Q" principle will be helpful for designing clinically transformative mRNA vectors with excellent storage stability and delivery efficiency.

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Experimental and theoretical study on the interactions between dopamine hydrochloride and nicotinamide

Zhai

Peng

Liu

et al. 2019

Journal of Molecular Structure

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A computational study of the interaction between dopamine and DNA/RNA nucleosides

Cited by 5 publications

References 56 publications

DNA Tetrahedral Nanocages as a Promising Nanocarrier for Dopamine Delivery in Neurological Disorders

DNA Tetrahedral Nanocages as a Promising Nanocarrier for Dopamine Delivery in Neurological Disorders

Rational Design of Polymeric mRNA Delivery Vectors to Achieve Excellent Room-Temperature Storage Stability and Delivery Efficiency

Experimental and theoretical study on the interactions between dopamine hydrochloride and nicotinamide

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