“…Previous studies investigating scaling and root planing have utilised dyes such as toluidine blue (Waerhaug 1975, Stambaugh et al 1981, Reinhardt et al 1985), malachite green (Schwarz et al 1993), methylene blue (Rabbani et al 1981, Eschler & Rapley 1991, Wylam et al 1993, Anderson et al 1996) and gentian violet (Poole et al 1984, Eaton et al 1985). In addition, a number of studies have used erythrosine to indicate the thoroughness of root surface debridement (Thornton & Garnick 1982, Eakle et al 1986, Walmsley et al 1988, Nosal et al 1988, Verdonschot et al 1990, Boyd et al 1992, Braun & Ciancio 1992). However, none of these studies have gone beyond macroscopic observations to analyse the nature of the stained material.…”