A computer-designed scaffold for bone regeneration within cranial defect using human dental pulp stem cells

Kwon, Doo Yeon; Kwon, Jin Seon; Park, Seung Hun; Park, Ji Hun; Jang, So Hee; Yin, Xiang Yun; Yun, Jeong‐Ho; Kim, Jae Ho; Min, Byoung Hyun; Lee, Jun Hee; Kim, Wan-Doo; Kim, Moon Suk

doi:10.1038/srep12721

Cited by 56 publications

(32 citation statements)

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“…This multipotency, in addition to their relative accessibility, made DPSCs an appealing source of cells for application in regenerative medicine [ 15 – 18 ]. In fact, several papers have proved their superiority in different aspects, including osteogenic differentiation [ 19 , 20 ], which supported their use for regeneration of craniofacial defects [ 21 , 22 ], as well as alveolar bone defects [ 23 , 24 ]. Additionally, the similar embryonic origins of dental pulp cells and periodontal cells [ 25 ] and their presence within protective layers of tooth structure have encouraged their use for periodontal tissue regeneration [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Osteogenic differentiation of dental pulp stem cells under the influence of three different materials

et al. 2015

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BackgroundRegeneration of periodontal tissues is a major goal of periodontal therapy. Dental pulp stem cells (DPSCs) show mesenchymal cell properties with the potential for dental tissue engineering. Enamel matrix derivative (EMD) and platelet-derived growth factor (PDGF) are examples of materials that act as signaling molecules to enhance periodontal regeneration. Mineral trioxide aggregate (MTA) has been proven to be biocompatible and appears to have some osteoconductive properties. The objective of this study was to evaluate the effects of EMD, MTA, and PDGF on DPSC osteogenic differentiation.MethodsHuman DPSCs were cultured in medium containing EMD, MTA, or PDGF. Control groups were also established. Evaluation of the achieved osteogenesis was carried out by computer analysis of alkaline phosphatase (ALP)-stained chambers, and spectrophotometric analysis of alizarin red S-stained mineralized nodules.ResultsEMD significantly increased the amounts of ALP expression and mineralization compared with all other groups (P < 0.05). Meanwhile, MTA gave variable results with slight increases in certain differentiation parameters, and PDGF showed no significant increase in the achieved differentiation.ConclusionsEMD showed a very strong osteogenic ability compared with PDGF and MTA, and the present results provide support for its use in periodontal regeneration.

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Section: Introductionmentioning

confidence: 99%

Osteogenic differentiation of dental pulp stem cells under the influence of three different materials

et al. 2015

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“…Our previous work demonstrated the controllable biodegradability of PCLA over a period ranging from days to a few weeks [13,14]. The degradation can be controlled by varying attributes such as the molecular weight and composition of polyesters.…”

Section: Resultsmentioning

confidence: 99%

“…In previously published work, we reported the controllable biodegradability and excellent biocompatibility of a poly( ε -caprolactone-co- l -lactide) (PCLA) random copolymer [13,14,15]. The PCLA can ideally degrade over a period ranging from days to a few weeks [13].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System

Lee

Park

et al. 2016

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Recently, electrospinning technology has been widely used as a processing method to make nanofiber sheets (NS) for biomedical applications because of its unique features, such as ease of fabrication and high surface area. To develop a sustained dexamethasone (Dex) delivery system, in this work, poly(ε-caprolactone-co-l-lactide) (PCLA) copolymer with controllable biodegradability was synthesized and further utilized to prepare electrospun Dex-loaded NS using water-insoluble Dex (Dex(b)) or water-soluble Dex (Dex(s)). The Dex-NS obtained by electrospinning exhibited randomly oriented and interconnected fibrillar structures. The in vitro and in vivo degradation of Dex-NS was confirmed over a period of a few weeks by gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). The evaluation of in vitro and in vivo Dex(b) and Dex(s) release from Dex-NS showed an initial burst of Dex(b) at day 1 and, thereafter, almost the same amount of release as Dex(b) for up to 28 days. In contrast, Dex(s)-NS exhibited a small initial burst of Dex(s) and a first-order releasing profile from Dex-NS. In conclusion, Dex-NS exhibited sustained in vitro and in vivo Dex(s) release for a prolonged period, as well as controlled biodegradation of the NS over a defined treatment period.

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“…To study the local bone formation using hDPSCs and SHED, cranial defects were created in rats on 11 manuscri-pts, 25 , 35 , 40 , 41 , 44 , 48 , 49 , 51 , 55 , 56 in mice on 4 manuscripts, 17 , 42 , 59 , 72 and in only one manuscript they were made on rabbits. 61 Furthermore, 10 studies created maxillary and mandibular bone defects 29 , 45 , 46 , 52 , 53 , 58 , 60 , 62 , 65 , 69 to assess the potential of hDPSCs for bone tissue engineering.…”

Section: The Experimental Designsmentioning

confidence: 99%

The use of human dental pulp stem cells for in vivo bone tissue engineering: A systematic review

et al. 2018

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Dental pulp represents a promising and easily accessible source of mesenchymal stem cells for clinical applications. Many studies have investigated the use of human dental pulp stem cells and stem cells isolated from the dental pulp of human exfoliated deciduous teeth for bone tissue engineering in vivo. However, the type of scaffold used to support the proliferation and differentiation of dental stem cells, the animal model, the type of bone defect created, and the methods for evaluation of results were extremely heterogeneous among these studies conducted. With this issue in mind, the main objective of this study is to present and summarize, through a systematic review of the literature, in vivo studies in which the efficacy of human dental pulp stem cells and stem cells from human exfoliated deciduous teeth (SHED) for bone regeneration was evaluated. The article search was conducted in PubMed/MEDLINE and Web of Science databases. Original research articles assessing potential of human dental pulp stem cells and SHED for in vivo bone tissue engineering, published from 1984 to November 2017, were selected and evaluated in this review according to the following eligibility criteria: published in English, assessing dental stem cells of human origin and evaluating in vivo bone tissue formation in animal models or in humans. From the initial 1576 potentially relevant articles identified, 128 were excluded due to the fact that they were duplicates and 1392 were considered ineligible as they did not meet the inclusion criteria. As a result, 56 articles remained and were fully analyzed in this systematic review. The results obtained in this systematic review open new avenues to perform bone tissue engineering for patients with bone defects and emphasize the importance of using human dental pulp stem cells and SHED to repair actual bone defects in an appropriate animal model.

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A computer-designed scaffold for bone regeneration within cranial defect using human dental pulp stem cells

Cited by 56 publications

References 50 publications

Osteogenic differentiation of dental pulp stem cells under the influence of three different materials

Osteogenic differentiation of dental pulp stem cells under the influence of three different materials

Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System

The use of human dental pulp stem cells for in vivo bone tissue engineering: A systematic review

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