A computer image processing system for quantification of zebrafish behavior

Kato, Satoru; Nakagawa, Takashi; Ohkawa, Makio; Muramoto, Ken-ichiro; Oyama, Osamu; Watanabe, Akihito; Nakashima, Hiroshi; Nemoto, Tetsu; Sugitani, Kayo

doi:10.1016/j.jneumeth.2003.09.028

Cited by 90 publications

(59 citation statements)

References 11 publications

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“…27,31,[50][51][52][53][54][55][56][57][58] For example, zebrafish swimming can be quantified using video-tracking software assessing velocity, distance traveled, and time spent moving during the trial. 11,29,36,[59][60][61][62][63][64][65] Recently developed software tools enable automated quantification of complex zebrafish group behavior, such as shoaling. 11 Mounting evidence also supports the value of three-dimensional (3D) analysis of zebrafish behavior, demonstrating its high sensitivity to various experimental manipulations.…”

Section: Glossarymentioning

confidence: 99%

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

et al. 2013

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Zebrafish (Danio rerio) are rapidly gaining popularity in translational neuroscience and behavioral research. Physiological similarity to mammals, ease of genetic manipulations, sensitivity to pharmacological and genetic factors, robust behavior, low cost, and potential for high-throughput screening contribute to the growing utility of zebrafish models in this field. Understanding zebrafish behavioral phenotypes provides important insights into neural pathways, physiological biomarkers, and genetic underpinnings of normal and pathological brain function. Novel zebrafish paradigms continue to appear with an encouraging pace, thus necessitating a consistent terminology and improved understanding of the behavioral repertoire. What can zebrafish 'do', and how does their altered brain function translate into behavioral actions? To help address these questions, we have developed a detailed catalog of zebrafish behaviors (Zebrafish Behavior Catalog, ZBC) that covers both larval and adult models. Representing a beginning of creating a more comprehensive ethogram of zebrafish behavior, this effort will improve interpretation of published findings, foster cross-species behavioral modeling, and encourage new groups to apply zebrafish neurobehavioral paradigms in their research. In addition, this glossary creates a framework for developing a zebrafish neurobehavioral ontology, ultimately to become part of a unified animal neurobehavioral ontology, which collectively will contribute to better integration of biological data within and across species.

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Section: Glossarymentioning

confidence: 99%

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

et al. 2013

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“…Several platforms for automated quantitative analysis of behavior are now available [19,[58][59][60][61][62], making robust quantitation readily accessible without a large up-front investment in programming.…”

Section: Prospectivesmentioning

confidence: 99%

The neurogenetic frontier--lessons from misbehaving zebrafish

Burgess

Granato²

2008

Briefings in Functional Genomics and Proteomics

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One of the central questions in neuroscience is how refined patterns of connectivity in the brain generate and monitor behavior. Genetic mutations can influence neural circuits by disrupting differentiation or maintenance of component neuronal cells or by altering functional patterns of nervous system connectivity. Mutagenesis screens therefore have the potential to reveal not only the molecular underpinnings of brain development and function, but to illuminate the cellular basis of behavior. Practical considerations make the zebrafish an organism of choice for undertaking forward genetic analysis of behavior. The powerful array of experimental tools at the disposal of the zebrafish researcher makes it possible to link molecular function to neuronal properties that underlie behavior. This review focuses on specific challenges to isolating and analyzing behavioral mutants in zebrafish.

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“…To quantify the restoration of motor function, behavioral analysis was performed with a slight modification of previously described method (Kato et al, 1996;Kato et al, 2004;Kaneda et al, 2008). In brief, a single zebrafish was placed in a water tank (370 mm × 240 mm, water depth = 80 mm) and its spontaneous movement was captured by two video cameras (EVI-1011; Sony, Tokyo, Japan) placed both on the top and at the side of the tank for 5 min at 30 frames/sec.…”

Section: Behavioral Analysismentioning

confidence: 99%

“…In brief, a single zebrafish was placed in a water tank (370 mm × 240 mm, water depth = 80 mm) and its spontaneous movement was captured by two video cameras (EVI-1011; Sony, Tokyo, Japan) placed both on the top and at the side of the tank for 5 min at 30 frames/sec. The position of the fish in each frame was determined, and the three-dimensional coordination of the fish was then reconstructed by combining two images (from the top and side view) with previously developed software (Kato et al, 1996;Kato et al, 2004;Kaneda et al, 2008) on a computer (Precision 490; Dell, Round Rock, TX, USA). The total distance of swimming was calculated as described in Kato et al (1996).…”

Section: Behavioral Analysismentioning

confidence: 99%

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Upregulation of anti-apoptotic factors in upper motor neurons after spinal cord injury in adult zebrafish

Ogai

Hisano

Mawatari

et al. 2012

Neurochemistry International

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Unlike mammals, fish motor function can recover within 6-8 weeks after spinal cord injury (SCI). The motor function of zebrafish is regulated by dual control; the upper motor neurons of the brainstem and motor neurons of the spinal cord. In this study, we aimed to investigate the framework behind the regeneration of upper motor neurons in adult zebrafish after SCI. In particular, we investigated the cell survival of

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A computer image processing system for quantification of zebrafish behavior

Cited by 90 publications

References 11 publications

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

The neurogenetic frontier--lessons from misbehaving zebrafish

Upregulation of anti-apoptotic factors in upper motor neurons after spinal cord injury in adult zebrafish

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