A concise review of applications of micellar liquid chromatography to study biologically active compounds

Stępnik, Katarzyna

doi:10.1002/bmc.3741

Cited by 21 publications

(14 citation statements)

References 113 publications

(75 reference statements)

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“…Surfactant based mobile phase are considered a green non‐organic mobile phase because of their lower cost and toxicity and higher biodegradability with respect to hydro‐organic mobile phases. Literature revealed reviews (i) on theoretical treatment, optimization strategy and applications of MLC to the determination of different groups of drug substances, [106] and (ii) on specific examples of application of MLC as a method for in vitro determination of biological activity, [107] and a report on HPLC analysis of seven tricyclic anti‐depressants (TCAs), and propranolol and alprenolol to determine retention behavior, selectivity, and resolution capability of the column in a chromatographic separation; [108] these reports, including the literature cited therein, did not show any attempt for enantioseparation of β‐adrenolytics using MLC.…”

Section: Enantioseparation By Indirect Approachmentioning

confidence: 99%

′Ab Ovo′ Chiral Phases and Chiral Reagents for Liquid Chromatographic Separation and Isolation of Enantiomers

Bhushan

2022

The Chemical Record

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The de-novo approach of mixing chirally pure reagents or Cu(II)-L-amino acid complexes in the slurry of silica gel for preparing TLC plates was reported from author's laboratory and was successful for separation and isolation of enantiomers. Using high molar absorptivity molecules, e. g., 1,5-difluoro-2,4-dinitrobenzene and cyanuric chloride, more than 38 new chiral derivatizing reagents were synthesized in our laboratory by straightforward nucleophilic substitution with simple chiral auxiliaries. Besides, (S)-naproxen, (S)-ketoprofen, and (S)-levofloxacin were used as chiral platforms. A conceptual approach using both achiral phases in chromatography for enantioseparation was also adopted. 1 H NMR and DFT based software were used to explain structures of non-covalent and covalent diastereomeric pairs and determination of configuration and separation mechanism. The methods can be easily used to determine and control enantiomeric purity with advantages over a variety of commercial chiral phases.

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Section: Enantioseparation By Indirect Approachmentioning

confidence: 99%

′Ab Ovo′ Chiral Phases and Chiral Reagents for Liquid Chromatographic Separation and Isolation of Enantiomers

Bhushan

2022

The Chemical Record

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“…For their analysis, the authors used the mobile phase consisting of SDS, propanol‐1 and triethylamine. The pH of the mobile phase in both cases was set at 3.5, the separation of the analyzed compounds was carried out on C18 columns using fluorescence detection [4–69].…”

Section: Micellar Liquid Chromatography Applicationsmentioning

confidence: 99%

“…The most widely used stationary phase in MLC is the alkyl-bonded C18 column, but other columns like C8 or cyanopropyl may be selected. The surfactants SDS, CTAB, Brij-35 or Tween-20 very strongly modifies the alkyl-bonded phase when incorporated into the mobile phase [40,55]. They adsorb on the porous RPLC packing and drastically affect the chromatographic retention, which leads to change in various surface properties of the stationary phase like structure, polarity, surface area and pore volume.…”

Section: Micellar Liquid Chromatography Applicationsmentioning

confidence: 99%

Analytical capabilities of micellar liquid chromatography and application to residue and contaminant analysis: A review

Patyra

Kwiatek

2021

J of Separation Science

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Green chemistry is the use of chemistry to reduce or eliminate the use of generation of feedstocks, products, by‐products, solvents, reagents, etc. that are hazardous to human health or the environment. One of the branches of green chemistry is micellar liquid chromatography. Micellar liquid chromatography is a reversed‐phase liquid chromatographic mode with mobile phases containing a surfactant above its critical micellar concentration. The applications of micellar liquid chromatography for the determination of numerous compounds in pharmaceutical formulation, biological samples, food, environmental samples, and feeds have been growing rapidly. Micellar liquid chromatography technique has several advantages over other chromatographic techniques. Its main advantage is the small amount of organic modifiers used such as acetonitrile and methanol and the safety and recyclability of the mobile phase. In our work, we discuss the development of “green chemistry” and present what micellar liquid chromatography is. This article presents application methods with the use of micellar liquid chromatography for analysis on antibacterial substances, melamine, biogenic amines, plant protection products, flavonoids, as well as peptides in biological matrices such as milk, eggs, tissues, honey, and feed.

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“…The surfactant‐based mobile phase in chromatography allows moderate interactions between the column and the analyte during separation, and these interactions can be controlled by varying the concentration of the surfactant. Earlier, combinations of a single or mixed surfactant‐based aqueous solution with organic solvents in chromatography were used to establish a variety of biological and organic product samples (Alwera & Bhushan, 2017a; Esteve‐Romero, Carda‐Broch, Gil‐Agustí, Capella‐Peiró, & Bose, 2005; Ruiz‐Angel, Peris‐García, & García‐Alvarez‐Coque, 2015; Stępnik, 2017). Surfactants are eco‐friendly, nontoxic, nonvolatile, and economical, and their aqueous solution as an eluent in LC favors green development.…”

Section: Introductionmentioning

confidence: 99%

Micellar liquid chromatographic green enantioseparation of racemic amino alcohols and determination of elution order

Alwera

Sehlangia

Alwera

2020

Biomedical Chromatography

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A micellar liquid chromatographic method was developed for the green enantioseparation of racemic amino alcohols using an aqueous solution of the mixed surfactants as an alternative for organic solvents. In this study, the derivatives of the amino alcohols were synthesized using highly reactive chiral esters of (S)‐levofloxacin (Lfx) under microwave conditions, and an aqueous solution of the surfactants (Brij‐35 and SDS) was used for the enantioseparation of the synthesized diastereomeric derivatives (DDs) of amino alcohols using reversed‐phase HPLC. The activated ester of Lfx was synthesized by reacting with N‐hydroxybenzotriazole and characterized using UV, IR, 1H NMR, high‐resolution mass spectrometry, and elemental analysis. The DDs of racemic amino alcohols were separated on a C18 column using micellar LC. Chromatographic conditions were optimized by varying the concentration of the surfactants in aqueous solution and by varying the concentration and pH of the buffer. The green assessment score was calculated for the developed method (score: 82, an excellent green method). In addition, the density functional theory calculations were performed to develop the lowest energy‐optimized structures of DDs. The method was validated according to the International Conference of Harmonization guidelines, and the retention factor (k), selectivity factor (α), resolution factor (RS), limit of detection (0.198 ng mL−1 or 0.291 pM mL−1), and limit of quantification (0.594 ng mL−1 or 0.873 pM mL−1) were calculated.

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A concise review of applications of micellar liquid chromatography to study biologically active compounds

Cited by 21 publications

References 113 publications

′Ab Ovo′ Chiral Phases and Chiral Reagents for Liquid Chromatographic Separation and Isolation of Enantiomers

′Ab Ovo′ Chiral Phases and Chiral Reagents for Liquid Chromatographic Separation and Isolation of Enantiomers

Analytical capabilities of micellar liquid chromatography and application to residue and contaminant analysis: A review

Micellar liquid chromatographic green enantioseparation of racemic amino alcohols and determination of elution order

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