Fruit and vegetable waste is an important kind of kitchen waste, its production can not be underestimated. The volatile fatty acids (VFAs) produced can be used as an added carbon source for microbial fermentation. This paper studied the effect of volatile fatty acids in the process of spring anaerobic hydrolysis, and investigated the concentration and composition of VFAs and the conversion rate of VS (volatile solid) over time. The results showed that in the intermittent mode of pH 6 and temperature of 35℃, the acid production of fruit and vegetable waste in spring was good, and the conversion rate of VFAs was 0.36g ·(gVfed ) -1.