A Concise Review on Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Personalized Regenerative Medicine

Pushp, Pallavi; Rodrigues, Carlos Alberto Chirano; Ferreira, Frederico Castelo; Gupta, Mukesh Kumar

doi:10.1007/s12015-020-10061-2

Cited by 19 publications

(11 citation statements)

References 305 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PSCs are primarily embryonic stem cells (ESCs) that are derived from an inner-cell mass of the preimplanted blastocyst or from primordial germ cells of the early embryos, and induced pluripotent stem cells (iPSCs) that are derived from somatic cells by using reprogramming factors and resemble ESCs in many ways. Although use of these cells faces ethical and safety concerns [ 8 ], they are widely studied for their superior differentiation potential over that of adult stem cells, and can hopefully be used for various therapeutic purposes in regenerative medicine in the future [ 9 ]. However, as early work tried to obtain cardiomyocytes from PSCs via different methods, such as the use of exogenous cytokines, growth factors, and extracellular matrix [ 10 ], these pluripotent stem cell-derived cardiomyocytes (PSC-CMs) turned out to be significantly more immature compared to adult cardiomyocytes and exhibit a fetal-like phenotype [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Promoted by Mechanical Stretch

Zhou

2021

Med Sci Monit

View full text Add to dashboard Cite

Stem cells have significant potential use in tissue regeneration, especially for treating cardiac diseases because of their multi-directional differentiation capability. By mimicking the in vivo physiological environment of native cardiomyocytes during their development and maturation, researchers have been able to induce pluripotent stem cell-derived cardiomyocytes (PSC-CMs) at high purity. However, the phenotype of these PSC-CMs is immature compared with that of adult cardiomyocytes. Various strategies have been explored to improve the maturity of PSC-CMs, such as long-term culturing, mechanical stimuli, chemical stimuli, and combinations of these strategies. Among these strategies, mechanical stretch as a key mechanical stimulus plays an important role in PSC-CM maturation. In this review, the optimal parameters of mechanical stretch, the effects of mechanical stretch on maturation of PSC-CMs, underlying molecular mechanisms as well as existing problems are discussed. Mechanical stretch is a powerful approach to promote the maturation of SC-CMs in terms of morphology, structure, and functionality. Nonetheless, further research efforts are needed to reach a satisfactory standard for clinical applications of PSC-CMs in treating cardiac diseases.

show abstract

Section: Introductionmentioning

confidence: 99%

Recent Advances in Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Promoted by Mechanical Stretch

Zhou

2021

Med Sci Monit

View full text Add to dashboard Cite

show abstract

“…Mouse and human fibroblasts can be successfully converted into iPSCs by the overexpression of pluripotencyrelated transcription factors Oct4, Sox2, Klf4, and c-Myc [139,140] (Figure 3a). As iPSCs are generated from cells derived from patients' own body, iPSCs-derived cardiomyocytes (iPSCs-CM) are less prone to immune rejection, and there are no ethical concerns involved [141]. Under in vitro conditions, iPSCs can be pushed towards a cardiac fate the same way as ESCs, either through the formation of embryoid bodies or monolayer culture [141].…”

Section: Delivery Of De Novo Cardiomyocytes From Differentiated Escs/...mentioning

confidence: 99%

“…As iPSCs are generated from cells derived from patients' own body, iPSCs-derived cardiomyocytes (iPSCs-CM) are less prone to immune rejection, and there are no ethical concerns involved [141]. Under in vitro conditions, iPSCs can be pushed towards a cardiac fate the same way as ESCs, either through the formation of embryoid bodies or monolayer culture [141]. The use of small molecules has also been extensively exploited to increase the efficiency of cardiac differentiation.…”

Section: Delivery Of De Novo Cardiomyocytes From Differentiated Escs/...mentioning

confidence: 99%

Metabolic Determinants in Cardiomyocyte Function and Heart Regenerative Strategies

et al. 2022

View full text Add to dashboard Cite

Heart disease is the leading cause of mortality in developed countries. The associated pathology is characterized by a loss of cardiomyocytes that leads, eventually, to heart failure. In this context, several cardiac regenerative strategies have been developed, but they still lack clinical effectiveness. The mammalian neonatal heart is capable of substantial regeneration following injury, but this capacity is lost at postnatal stages when cardiomyocytes become terminally differentiated and transit to the fetal metabolic switch. Cardiomyocytes are metabolically versatile cells capable of using an array of fuel sources, and the metabolism of cardiomyocytes suffers extended reprogramming after injury. Apart from energetic sources, metabolites are emerging regulators of epigenetic programs driving cell pluripotency and differentiation. Thus, understanding the metabolic determinants that regulate cardiomyocyte maturation and function is key for unlocking future metabolic interventions for cardiac regeneration. In this review, we will discuss the emerging role of metabolism and nutrient signaling in cardiomyocyte function and repair, as well as whether exploiting this axis could potentiate current cellular regenerative strategies for the mammalian heart.

show abstract

“…As a result, a question about the experimental efficiency of iPSCs arises. Malignancies and oncogenes can also be induced in the host when employing viral vectors [23,30]. Maza et al found that lowering the Mbd3 gene can enable all cells to acquire pluripotency, which is a key hurdle to employing iPSCs in clinical practice [31].…”

Section: B Induced Pluripotent Stem Cells (Ipscs)mentioning

confidence: 99%

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Sharma

Dash

Govarthanan

et al. 2021

Cells

View full text Add to dashboard Cite

Myocardium Infarction (MI) is one of the foremost cardiovascular diseases (CVDs) causing death worldwide, and its case numbers are expected to continuously increase in the coming years. Pharmacological interventions have not been at the forefront in ameliorating MI-related morbidity and mortality. Stem cell-based tissue engineering approaches have been extensively explored for their regenerative potential in the infarcted myocardium. Recent studies on microfluidic devices employing stem cells under laboratory set-up have revealed meticulous events pertaining to the pathophysiology of MI occurring at the infarcted site. This discovery also underpins the appropriate conditions in the niche for differentiating stem cells into mature cardiomyocyte-like cells and leads to engineering of the scaffold via mimicking of native cardiac physiological conditions. However, the mode of stem cell-loaded engineered scaffolds delivered to the site of infarction is still a challenging mission, and yet to be translated to the clinical setting. In this review, we have elucidated the various strategies developed using a hydrogel-based system both as encapsulated stem cells and as biocompatible patches loaded with cells and applied at the site of infarction.

show abstract

A Concise Review on Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Personalized Regenerative Medicine

Cited by 19 publications

References 305 publications

Recent Advances in Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Promoted by Mechanical Stretch

Recent Advances in Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Promoted by Mechanical Stretch

Metabolic Determinants in Cardiomyocyte Function and Heart Regenerative Strategies

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Contact Info

Product

Resources

About