A condensates-to-VPS41-associated phagic vacuoles conversion pathway controls autophagy degradation in plants

Jiang, Dong; He, Yilin; Li, Hailin; Dai, Liufeng; Sun, Bingyan; Yang, Lianming; Pang, Lei; Cao, Zhiran; Liu, Yu; Gao, Jiayang; Zhang, Yi; Jiang, Liwen; Li, Ruixi

doi:10.1016/j.devcel.2024.07.010

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2024

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Cited by 3 publications

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HOPping to the vacuole: Autophagosome and late endosomes combine to control plant autophagosome degradation

Dragwidge,

Bernard

2024

Developmental Cell

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HOPping to the vacuole: Autophagosome and late endosomes combine to control plant autophagosome degradation

Dragwidge,

Bernard

2024

Developmental Cell

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An interplay between biomolecular condensates and SNARE proteins regulates plant autophagy

Li,

Pang

2024

Autophagy

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Biomolecular condensation of ERC1 recruits ATG8 and NBR1 to drive autophagosome formation for plant heat tolerance

Chung,

Zhao,

Law

et al. 2024

Preprint

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Macroautophagy (hereafter autophagy) is essential for cells to respond to nutrient stress by delivering cytosolic contents to vacuoles for degradation via the formation of a multi-layer vesicle named autophagosome. A set of autophagy-related (ATG) regulators are recruited to the phagophore assembly site for the initiation of phagophore, as well as its expansion and closure and subsequent delivery into the vacuole. However, it remains elusive that how the phagophore assembly is regulated under different stress conditions. Here, we described an unknownArabidopsis (Arabidopsis thaliana)cytosolic ATG8-interaction protein family (ERC1/2), that binds ATG8 and NBR1 to promote autophagy. ERC1 proteins translocate to the phagophore membrane and develop into classical ring-like autophagosomes upon autophagic induction. However, ERC1 proteins form large droplets together with ATG8e proteins when in the absence of ATG8 lipidation activity. We described the property of these structures as phase-separated membraneless condensates by solving thein vivoorganization with spatial and temporal resolution. Moreover, ERC1 condensates elicits a strong recruitment of the autophagic receptor NBR1. Loss of ERC1 suppressed NBR1 turnover and attenuated plant tolerance to heat stress condition. This work provides novel insights into the mechanical principle of phagophore initiation via an unreported ERC1-mediated biomolecular condensation for heat tolerance inArabidopsis.

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A condensates-to-VPS41-associated phagic vacuoles conversion pathway controls autophagy degradation in plants

Cited by 3 publications

References 61 publications

HOPping to the vacuole: Autophagosome and late endosomes combine to control plant autophagosome degradation

HOPping to the vacuole: Autophagosome and late endosomes combine to control plant autophagosome degradation

An interplay between biomolecular condensates and SNARE proteins regulates plant autophagy

Biomolecular condensation of ERC1 recruits ATG8 and NBR1 to drive autophagosome formation for plant heat tolerance

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