A conditional female lethal system for genetic suppression of the global fruit crop pest <scp><i>Drosophila suzukii</i></scp>

Li, Fang; Yamamoto, Akihiko; Belikoff, Esther J.; Berger, Amy; Griffith, Emily H.; Scott, Maxwell J.

doi:10.1002/ps.6530

Cited by 21 publications

(22 citation statements)

References 51 publications

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“…In this study, the FL19 transgene reported recently (Li et al, 2021) was remobilized to new locations on the third and X chromosomes. As expected, the third chromosome jumps were relatively short with the most distant transgene less than 70 kb a Total count of offspring from three independent vials of flies except for the homozygous chromosome 3 and X + FL19 lines where the data is from the productivity experiment shown in from the original FL19 location.…”

Section: Discussionmentioning

confidence: 95%

“…The relative humidity in the lab was between 20% and 50% and the lights were on for about 12 h on most days. The original wild-type colony was established from infested fruit collected from a field in North Carolina in 2011 ( Burrack et al, 2013 ) and the initial FL19 strain was previously made by piggyBac -mediated germline transformation of this wild type strain ( Li et al, 2021 ). The wild type colony was periodically genetically augmented with flies collected in North Carolina ( Elsensohn et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The original FL19 male-only strain was made by piggyBac transposase-mediated germline transformation (Li et al, 2021).…”

Section: Transposition Of the Fl19 Transgene To New Chromosomal Locat...mentioning

confidence: 99%

“…The female-specific tTA overexpression systems were functional in D. melanogaster , indicating that the screwworm and C. capitata tra introns were correctly spliced in D. melanogaster ( Fu et al, 2007 ; Li et al, 2014 ). We recently developed the FL19 D. suzukii fsRIDL strain that had a female-specific tTA overexpression gene and a tTA activated hid gene in a single construct ( Li et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Subsequently, repeated releases of an excess of fertile fsRIDL males led to eradication of the populations ( Ant et al, 2012 ; Leftwich et al, 2014 ; Harvey-Samuel et al, 2015 ). With D. suzukii , repeated releases of FL19 males (approximately 10–13:1 ratio) led to a sharp reduction in egg production in the first month and by 8 weeks the test cages had stopped laying eggs ( Li et al, 2021 ). Males from a fsRIDL strain of the diamondback moth, Plutella xylostella , have been tested in cages ( Harvey-Samuel et al, 2015 ) and in the open field ( Shelton et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Additional Drosophila suzukii Male-Only Strains Generated Through Remobilization of an FL19 Transgene

Yamamoto

Yadav

Scott

2022

Front. Bioeng. Biotechnol.

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Drosophila suzukii (D. suzukii) (Matsumura, 1931; Diptera: Drosophilidae), also known as spotted wing Drosophila, is a worldwide pest of fruits with soft skins such as blueberries and cherries. Originally from Asia, D. suzukii is now present in the Americas and Europe and has become a significant economic pest. Growers largely rely on insecticides for the control of D. suzukii. Genetic strategies offer a species-specific environmentally friendly way for suppression of D. suzukii populations. We previously developed a transgenic strain of D. suzukii that produced only males on a diet that did not contain tetracycline. The strain carried a single copy of the FL19 construct on chromosome 3. Repeated releases of an excess of FL19 males led to suppression of D. suzukii populations in laboratory cage trials. Females died as a consequence of overexpression of the tetracycline transactivator (tTA) and tTA-activated expression of the head involution defective proapoptotic gene. The aim of this study was to generate additional male-only strains that carried two copies of the FL19 transgene through crossing the original line with a piggyBac jumpstarter strain. Males that carried either two chromosome 3 or a singleX-linked transgene were identified through stronger expression of the red fluorescent protein marker gene. The brighter fluorescence of the X-linked lines was likely due to dosage compensation of the red fluorescent protein gene. In total, four X-linked lines and eleven lines with two copies on chromosome 3 were obtained, of which five were further examined. All but one of the strains produced only males on a diet without tetracycline. When crossed with wild type virgin females, all of the five two copy autosomal strains examined produced only males. However, the single copy X-linked lines did not show dominant female lethality. Five of the autosomal lines were further evaluated for productivity (egg to adult) and male competition. Based on these results, the most promising lines have been selected for future population suppression experiments with strains from different geographical locations.

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Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

“…The original FL19 male-only strain was made by piggyBac transposase-mediated germline transformation (Li et al, 2021).…”

Section: Transposition Of the Fl19 Transgene To New Chromosomal Locat...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Additional Drosophila suzukii Male-Only Strains Generated Through Remobilization of an FL19 Transgene

Yamamoto

Yadav

Scott

2022

Front. Bioeng. Biotechnol.

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Radioactivity and GMO‐Free Sterile Insect Technology for the Sustainable Control of the Invasive Pest Drosophila suzukii

Abdelhafiz,

Gerth,

Claussen

et al. 2024

Advanced Biology

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Drosophila suzukii (D. suzukii), commonly known as the spotted wing drosophila, is a highly invasive crop pest that is difficult to control using chemical insecticides. To address the urgent need for alternative and more sustainable control strategies, the sterile insect technique (SIT) is improved, which involves the release of sterilized male insects to mate with fertile conspecifics, thereby reducing the size of the pest population in the subsequent generation. The three critical aspects that influence the success of SIT programs in D. suzukii are addressed. First, an accurate and nondestructive method is established to determine the sex of individual insects based on the differential weight of male and female pupae. Second, conditions for X‐ray sterilization are systematically tested and an optimal dose (90 kV/40 Gy) is identified that ensures the efficient production of sterile D. suzukii for release. Finally, the inherent thermosensitivity of D. suzukii males is exploited to develop a temperature‐based sterilization technique, offering an alternative or additional SIT method for this pest. These advances will contribute to the development of a comprehensive and effective strategy for the management of D. suzukii populations, reducing their impact on agriculture and helping to safeguard crop yields.

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Maleness-on-the-Y (MoY) orthologue is a key regulator of male sex determination in Zeugodacus cucurbitae (Diptera: Tephritidae)

FAN¹,

MA²,

MA³

et al. 2023

Journal of Integrative Agriculture

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A conditional female lethal system for genetic suppression of the global fruit crop pest Drosophila suzukii

Cited by 21 publications

References 51 publications

Evaluation of Additional Drosophila suzukii Male-Only Strains Generated Through Remobilization of an FL19 Transgene

Evaluation of Additional Drosophila suzukii Male-Only Strains Generated Through Remobilization of an FL19 Transgene

Radioactivity and GMO‐Free Sterile Insect Technology for the Sustainable Control of the Invasive Pest Drosophila suzukii

Maleness-on-the-Y (MoY) orthologue is a key regulator of male sex determination in Zeugodacus cucurbitae (Diptera: Tephritidae)

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