A tuning fork is a special tool made of metal and shaped like the letter U with one handle. Tuning forks can produce certain frequencies; usually, the value is written on the handle. This study aims to investigate the relationship between temperature and changes in tuning fork frequency and model it using polynomial regression. This research uses laboratory experiments with tuning forks that have frequencies of 341.3 Hz, 426.5 Hz, and 512 Hz, then the temperature on the tuning fork is varied from 30C to 220C with a difference of 10C. The study's results adjusted R-Square values sequentially 0.94745, 0.99565, and 0.97721, which stated the relationship between temperature and frequency changes. The Adjusted R-Square value close to 1 means that changes in temperature on the tuning fork greatly influence changes in the frequency produced by the tuning fork. The polynomial regression model used is very suitable