A consensus genetic map of sorghum that integrates multiple component maps and high-throughput Diversity Array Technology (DArT) markers

Abstract

Background: Sorghum genome mapping based on DNA markers began in the early 1990s and numerous genetic linkage maps of sorghum have been published in the last decade, based initially on RFLP markers with more recent maps including AFLPs and SSRs and very recently, Diversity Array Technology (DArT) markers. It is essential to integrate the rapidly growing body of genetic linkage data produced through DArT with the multiple genetic linkage maps for sorghum generated through other marker technologies. Here, we report on the colinearity of six independent sorghum component maps and on the integration of these component maps into a single reference resource that contains commonly utilized SSRs, AFLPs, and high-throughput DArT markers.

Towards a unified genetic map for diploid roses

“…Therefore, the marker number and density in the ICM is only slightly higher than in the 94/1 map of Yan et al (2005) but the marker order is signiWcantly more reliable, because a much higher number of individuals (Vezzulli et al 2008) and higher number of recombination events was taken into account in the combined four populations. The length of the ICM is 530 cM, which is about 17% larger than the values from the recalculated single maps for 94/1, 97/7 and HW populations (Table 2) (Mace et al 2009) is in the range of the single population maps. Part of this increase may be due to an improved coverage of the ends of the chromosomes.…”

“…The lack of bridge markers in telomeric regions was also observed in sorghum with as many as 251 bridge markers for 4 of the 10 chromosomes (Mace et al 2009), and in red clover with 260 bridge markers on 7…”

Striga

“…In pearl millet, although marker aided-selection (MAS) has not been practiced yet in breeding for Striga resistance, there have been extensive efforts towards development of DNA-based markers including simple sequence repeat (SSR) Budak et al, 2003;Mariac et al, 2006;Qi et al 2001Qi et al , 2004Senthilvel et al, 2004Senthilvel et al, , 2008Jia et al, 2007;Yadav et al, 2007Yadav et al, , 2008Rajaram et al, 2013), diversity array technology (DArT) (Mace et al, 2008(Mace et al, , 2009Supriya et al, 2011) and single nucleotide polymorphism (SNP) (Bertin et al, 2005;Feltus et al, 2006;Kountche, 2013;Moumouni et al, 2015). In most of these studies, a number of genetic maps have been constructed using different crosses, mostly with the aim of mapping specific traits.…”

Simple sequence repeat markers associated with anthracnose and <i>Turcicum</i> leaf blight resistance in sorghum

“…All data were analysed using GenStat 12 th Version (VSN International Ltd., UK) (Steel and Torrie, 1997). Polymorphic SSRs were located in linkage groups 1, 2, 4, 5, 6 and 7 based on the sorghum map reported by Mace et al (2009).…”

“…DNA was isolated from two week old leaf tissues of the plants (Edwards et al, 1991). Fifty eight SSRs, from the consensus genetic map of sorghum obtained through Diversity Array Technology (DArT) was used (Mace et al (2009). These SSRs were synthesized at the Department of Molecular and Cellular Biology, University of Cape Town, South Africa.…”