A consensus map for Cucurbita pepo

Zraidi, Amine; Stift, Gertraud; Pachner, Martin; Shojaeiyan, Abdolali; Gong, Li; Lelley, T.

doi:10.1007/s11032-007-9098-6

Cited by 50 publications

(46 citation statements)

References 56 publications

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“…Distorted segregation was observed in 30 SNPs and 2 SSRs, a larger number than in the Pumpkin x Crookneck cross (Zraidi et al, 2007;Gong et al, 2008b), but lower than that reported in maps constructed from interspecific crosses (Brown and Myers, 2002). Grouped markers were especially observed in LG2 and LG5 .…”

Section: Genetic Map Of the Zucchini X Scallop Populationcontrasting

confidence: 56%

“…Los trabajos incluidos en la presente Tesis referentes a C. pepo incluyen, por una parte, la validación de parte de esta colección de EST-SSRs y su aplicación en un estudio de variabilidad en comparación con los SSRs genómicos previamente desarrollados por : "Genetic diversity of Spanish Cucurbita pepo landraces: an unexploited resource for summer squash breeding") y, por otra parte, la aplicación de 384 EST-SNPs para la construcción del primer mapa genético en el género basado en este tipo de marcadores (Esteras et al, 2012a: "High-throughput SNP genotyping in Cucurbita pepo for map construction and quantitative trait loci mapping"). Los mapas previos estaban basados principalmente en marcadores dominantes y poco transferibles a otras poblaciones (Lee et al, 1995y Brown y Myers, 2002, basados en el cruce interespecífico C. pepo x C. moschata; Zraidi et al, 2007), o basados en cruces intraespecíficos e inter-subespecíficos dentro de C. pepo), y sólo el publicado por incluía gSSRs, algunos de los cuales se anclan al mapa presentado aquí (Esteras et al, 2012a). La aplicación de estos marcadores de alta calidad no sólo ha impulsado los estudios de diversidad en la especie, incluyendo tipos comerciales y tradicionales, sino que ha permitido la puesta a punto de técnicas de genotipado de alto rendimiento como la plataforma GoldenGate de Illumina y un primer análisis de QTLs (Esteras et al, 2012a).…”

Section: Desarrollo De Rils Marcadores Codominantes Ssr Caps Y Snp unclassified

“…pepo and C. moschata. These markers show high interspecies transferability and have been used to construct the first published C. pepo map (Zraidi et al, 2007;Gong et al, 2008a, b). Stift et al (2004) Straightneck, ovifera gourds and wild forms.…”

Section: I3113 Molecular Markers Used In Genetic Diversity Studiesmentioning

confidence: 99%

“…ovifera cross (oil-seed Pumpkin x Italian Crookneck, respectively). These maps consisted mainly of RAPDs and Amplified Fragment Length Polymorphisms (AFLPs) (Zraidi and Lelley, 2004;Zraidi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Desarrollo y aplicación de herramientas genómicas para la mejora de especies cucurbitáceas por calidad y resistencia a enfermedades.

Gómez¹

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Melon (Cucumis melo) and summer squash (Cucurbita pepo) are economically important cucurbits cultivated worldwide. New, better-adapted, cultivars, resistant to pests and diseases, and varieties displaying better quality traits are required to optimize their production and to meet market demands. Crop breeding must be carried out efficiently and competitively, using the existing genetic knowledge and the molecular tools available for each species. The development of genomic tools to speed up Cucurbits breeding programs is the main objective of this Doctoral Thesis.Molecular markers are essential tools for the construction of genetic maps, for an efficient MAS selection, for QTL (Quantitative Trait Loci) analysis and mapping and for the development of new breeding populations. Molecular markers are also necessary tools for biodiversity analysis. High-quality markers, such as microsatellites (Simple Sequence Repeats, SSRs) and SNPs (Single Nucleotide Polymorphisms) have been developed and/or validated for both species in this Thesis. The generation of sequence information, required for the development of SSRs and SNPs has dramatically changed in the last 6 years due to the advent of new technological advances. In fact, a fairly complete melon transcriptome has been produced using high-throughput 454 sequencing (NGS, Next Generation Sequencing) and combining the newly produced ESTs with the previously existing Sanger sequences. The melon transcriptome characterization presented is the most complete to date (53.252 unigenes with over 63% of them annotated). The generation of large SSRs and SNPs collections for these species, by mapping the ESTs (Expressed Sequence Tags) against the new version of the transcriptome has been a great achievement starting a new era in melon breeding.The obtained collection of molecular markers (more than 3.000 SSRs and 38.000 SNPs) has allowed the construction of melon maps with increased marker density. 6Similar resources have been developed for the first time in C. pepo. SSRs identified in the C. pepo transcriptome sequencing project has been applied to localize landraces with favourable commercial traits and whose alleles might be exploited in breeding programs. Likewise, the usefulness of both EST-SSRs and genomic SSRs for depicting genetic relationships has been proved. On the other hand, the employment of the first SNP set identified for Cucurbita through a massive genotyping platform, GoldenGate, has been a success (90%). The genetic map obtained, with more than 300 SNPs, has allowed to map a large set of QTLs related to early flowering, femaleness tendency, shape, size and fruit color, and other traits of interest in breeding.Therefore, these works report the validation and application of previously developed markers and the generation of new genomic tools to speed up the breeding process of these crops. The paper-based format of this Thesis confirms the interest of the obtained results. 7 RESUMENEl melón (Cucumis melo) y el calabacín (Cucurbita pepo) son especies cucurbi...

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Section: Genetic Map Of the Zucchini X Scallop Populationcontrasting

confidence: 56%

Section: Desarrollo De Rils Marcadores Codominantes Ssr Caps Y Snp unclassified

Section: I3113 Molecular Markers Used In Genetic Diversity Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Desarrollo y aplicación de herramientas genómicas para la mejora de especies cucurbitáceas por calidad y resistencia a enfermedades.

Gómez¹

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“…molecular markers have been used in assessing genetic variation, in distinguishing cultivars, and in creating genetic maps (Brown and Myers 2002;Gong et al 2008;Lee et al 1995;Paris et al 2003;Zraidi et al 2007). The most recently published map of C. pepo covers approximately 86.8% of the genome (2n = 2x = 40) and contains 20 linkage groups with a map density of 2.9 cM (Gong et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Identification of molecular markers associated with resistance to squash silverleaf disorder in summer squash (Cucurbita pepo)

Kabelka

Young

2009

Euphytica

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Squash silverleaf (SSL), caused by the silverleaf whitefly [Bemisia argentifolii (formerly known as Bemisia tabaci Gennadius, B strain)], is an important physiological disorder that affects squash (Cucurbita spp.) by reducing yield potential. Breeding squash with resistance to SSL disorder can be facilitated by using marker-assisted selection (MAS). Resistance to SSL disorder, in Cucurbita pepo, is conferred by a single recessive gene (sl). The objective of this study was to identify molecular markers associated with resistance. A zucchini squash, SSL disorder resistant breeding line, 'Zuc76' (sl/sl) and a SSL disorder susceptible zucchini cultivar 'Black Beauty' (Sl/Sl) were screened with 1,152 randomly amplified polymorphic DNA (RAPD) primers and 432 simple sequence repeat (SSR) markers to identify polymorphisms. Using F 2 and BC 1 progeny segregating for SSL disorder resistance, three RAPD (OPC07, OPL07 and OPBC16) primers and one SSR (M121) marker were found associated with sl. Fragments amplified by RAPD primer OPC07 was linked in coupling phase to sl, whereas RAPD primer OPL07 was linked in repulsion phase. RAPD primer OPBC16 and SSR marker M121 were co-dominant. The allelic order of these loci was found to be M121-sl-OPC07-OPL07-OPBC16. The closest marker to sl is M121 with an estimated genetic distance of 3.3 cM. The markers identified in this study will be useful for breeding summer squash (C. pepo) for SSL disorder resistance derived from zucchini squash breeding line 'Zuc76'.

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Genetic Resources of Pumpkins and Squash, Cucurbita spp.

Paris¹

2016

Plant Genetics and Genomics: Crops and Models

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A consensus map for Cucurbita pepo

Cited by 50 publications

References 56 publications

Desarrollo y aplicación de herramientas genómicas para la mejora de especies cucurbitáceas por calidad y resistencia a enfermedades.

Desarrollo y aplicación de herramientas genómicas para la mejora de especies cucurbitáceas por calidad y resistencia a enfermedades.

Identification of molecular markers associated with resistance to squash silverleaf disorder in summer squash (Cucurbita pepo)

Genetic Resources of Pumpkins and Squash, Cucurbita spp.

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