A conserved residue in the P2X4 receptor has a nonconserved function in ATP recognition

Abstract

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Structural insights into the allosteric inhibition of P2X4 receptors

“…Plasmids were transfected into HEK293 cells by calcium phosphate transfection. Nystatinperforated patch-clamp recordings were performed 24-48 h after transfection at room temperature (25 ± 2 °C) [48][49][50] . Patch pipettes were pulled from glass capillaries by a two-stage puller (PC-100, Narishige, Japan), and the resistance was 3-5 megaohms (mΩ) when filled with the pipette solution containing (in mM) 75 K 2 SO 4 , 55 KCl, 5 MgSO 4 and 10 HEPES adjusted to pH 7.2.…”