A conserved tooth resorption mechanism in modern and fossil snakes

LeBlanc, Aaron R. H.; Palci, Alessandro; Anthwal, Neal; Tucker, Abigail S.; Araújo, Ricardo; Pereira, Miguel

doi:10.1038/s41467-023-36422-2

Cited by 7 publications

(3 citation statements)

References 52 publications

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“…Note that these small‐scaled wavy patterns may also correspond to an erosion front (as in Ørvig 1976, 1978b; LeBlanc et al . 2023). It is thus unclear for now whether and how the presence and shape or orientation of these scallops can be used to favour one developmental hypothesis over another.…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, as cases of erosion can be observed in odontodes (Ørvig 1976, 1978b), teeth (LeBlanc et al . 2023), and conodont elements (Shirley et al . 2018), the vertical unconformities could also be formed via erosion of the top orthodentine and secondary deposition of the capping tissue (illustrated as an irregular erosion front in Fig.…”

Section: Discussionmentioning

confidence: 99%

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Developmental models shed light on the earliest dental tissues, using Astraspis as an example

Houée,

Bardin,

Germain

et al. 2023

Palaeontology

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Fossils of extinct jawless vertebrates are pivotal to deciphering the evolutionary paths that led to the various forms of the vertebrate skeleton. For example, Pteraspidomorphs (stem‐gnathostomes), such as the Ordovician Astraspis, display some of the oldest remains of bony and ‘dental’ (dentine and enameloid) tissues. However, the identification of the very nature of these early mineralized tissues has been hampered by a lack of unambiguous diagnostic characters. As development is key to identifying the derivation of these tissues, we developed an integrative and generic histogenetic model, testing several ontogenetic scenarios. We illustrate our approach on the basis of the well‐preserved Astraspis samples and show how this can be used to infer key developmental features from extinct species. This study suggests that in the odontodes of Astraspis: (1) the initial curvature of the epithelium was close to the shape of the final external surface; (2) the mesenchymal cells differentiate synchronously in the whole inner periphery; and (3) the capping tissue was produced by both mesenchymal and epithelial cells (enameloid rather than enamel). Astraspis specimens also provide evidence of a dual growth periodicity, possibly homologous to Andresen and von Ebner growth lines observed in amniotes, suggesting this type of dual periodicity may be shared by most vertebrates. We estimated that an Astraspis odontode grew up in around 60–70 days at a rate of 0.5–5 μm/day. The new developmental approach proposed in this study could be a robust framework for critically evaluating the tissues of extinct taxa in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Developmental models shed light on the earliest dental tissues, using Astraspis as an example

Houée,

Bardin,

Germain

et al. 2023

Palaeontology

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“…Only 2 previous studies looked at the molecular basis of tooth shedding in reptiles (Fuenzalida et al 1999; LeBlanc et al 2023). These study employed Tartarate Acid Resistant Phosphatase staining (TRAP) to identify multinucleated, TRAP+ cells in Liolaemus gravenhorsti (lizard) and Pantherophis guttatus (snake).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic profiling of the adult reptilian dentition sheds light on the genes regulating indefinite tooth replacement

Henriquez

Flibotte

et al. 2022

Preprint

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The aim of this study is to profile the transcriptomes of teeth and the surrounding tissues of an adult lizard dentition (Eublepharis macularius) that is actively replacing teeth throughout life. Bulk RNAseq was used to compare teeth that are in function versus unerupted, developing teeth and single cell RNA-seq was carried out on jaw segments containing the dental forming tissues. In bulk RNAseq data, we found that functional teeth expressed genes involved in bone and tooth resorption. Indeed, multinucleated odontoclasts were abundant in tissue sections of functional teeth undergoing resorption. These are the first steps towards characterizing odontoclasts that participate in physiological resorption. Unexpectedly, chemotaxis gene SEMA3A was expressed within odontoblasts and in adjacent mesenchyme, confirmed using RNAscope. Semaphorins may be involved in regulating odontoclasts during tooth resorption. The scRNA-seq experiment successfully isolated dental mesenchyme and epithelial cells. We confirmed that some of these genes are expressed in the earliest tooth buds within the tooth forming field. In addition, we found evidence of convergent evolution in the tooth eruption trait. Geckos evolved a means for second generation teeth to communicate with the functional teeth. Instead of a dental follicle inducing an eruption pathway as in the mammal, the gecko and other squamate reptiles use the enamel organ of the successional teeth to trigger tooth resorption of the functional teeth, thus creating an eruption pathway. New molecules such as SEMA3A may also participate in this process. Future studies on the gecko will uncover the molecular basis of convergent evolution in the dentition.

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Tooth Formation

Berkovitz

Shellis

2023

The Teeth of Non-Mammalian Vertebrates

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A conserved tooth resorption mechanism in modern and fossil snakes

Cited by 7 publications

References 52 publications

Developmental models shed light on the earliest dental tissues, using Astraspis as an example

Developmental models shed light on the earliest dental tissues, using Astraspis as an example

Transcriptomic profiling of the adult reptilian dentition sheds light on the genes regulating indefinite tooth replacement

Tooth Formation

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