The purpose of this study was to investigate the effect of application time of colloidal platinum nanoparticles (CPN) on bond strength. Dentin surfaces were subjected to one of the following treatments: (A) Etching with 10% citric acid-3% FeCl3 solution (10-3 solution); (B) Etching with 10-3 solution followed by applying CPN as a primer solution for 10, 20, 30, or 60 seconds; and (C) Priming with CPN for 10, 20, 30, or 60 seconds followed by etching with 10-3 solution. An acrylic rod was bonded to each treated dentin surface using 4-META/MMA-TBB resin. Bonded specimens were sectioned into beams for microtensile bond strength testing. In groups (B) and (C), highest bond strength was obtained when dentin surfaces were treated with CPN for 30 seconds. This meant that the CPN primer solution either enhanced the penetration of resin into dentin or the degree of conversion of 4-META/MMA-TBB resin. Within the limitations of this study, treatment with 0.1 mN CPN primer solution followed by 20 seconds of water rinsing resulted in high bond strength.