A Consideration on the Role of HSP70 Appearing in the Periodontal Tissues due to Experimental Orthodontic Force

Abstract

We examined immunohistochemical expressions of HSP70 and p-HSP70 in the orthodontic periodontal tissues. In the control group, the HSP70 and p-HSP70 expression was observed in the periodontal ligament fibroblasts and that was kept in low levels. In the experimental group, the strong expression of HSP70 was detected according to over time. However, p-HSP70 expression was a bit delayed. The data suggests that HSP70 has been closely involved in the repair of tissue to maintain homeostasis of the periodontal tissues by the activation of periodontal ligament fibroblasts. Farthermore, the data also suggests that HSP70 act as a molecular chaperone of osteogenesis through an activation of osteoblasts.

HSP47 as a Possible Molecular Chaperone for the Collagen Synthesis in the Mouse Periodontal Ligament Cells due to Orthodontic Force

“…In the past, we carried out animal experiments to establish an immunohistological basis for orthodontic treatment. We investigated various proteins expressed in the mouse periodontal tissue after application of mechanical stress simulating orthodontic treatment [2][3][4][5][6][7][8][9][10][11]. However, cellular responses related to repair of the injurious effects of mechanical stress on PDL fibroblasts during corrective treatment have barely been investigated.…”

“…Expression of HSP peptides occurs within an extremely short time in PDL cells subjected to mechanical stress over time [2][3][4][5][6][8][9][10]. Accordingly, HSP47, which contributes to cell differentiation, is also expressed in a comparatively short time and changes in the intensity of HSP47 expression could be observed within 24 hours.…”

“…In orthodontic treatment, remodeling of the periodontal tissue is brought about by mechanical stress due to a correction device and, because of tissue responses to this mechanical stress, the tooth moves [1]. The periodontal tissue responds to different types of stimulus such as mechanical stress and inflammation, to maintain homeostasis, and expresses various proteins to bring about active remodeling of the periodontal tissue [2][3][4][5][6][7][8][9][10][11]. It is important to grasp the kinetics of various factors expressed in the periodontal tissue during orthodontic treatment, as a biochemical basis.…”

Immunohistochemical Expression of Osterix Appearing in the Mouse Orthodontic Periodontal Tension Sides

“…Furthermore, the osteogenesis related factors were also examined using immunohistochemical analysis, such as Runx2, Msx2 5-7) , BMP-2, BMP-4, Smad, p-Smad 8,9) and ALP. Furtheremore, HSPs and p-HSPs [10][11][12][13] were also examined on the orthodontic periodontal tension side.…”

“…Thus, some researchers have examined the immunohistochemical profile change of experimental orthodontic periodontal tissues using animal model. Examined data have been published regarding Runx2 and Msx2 [5][6][7] , BMPs and Smad 8,9) and HSPs [10][11][12][13] .…”