A constitutive and drought-responsive mRNA undergoes long-distance transport in pear (Pyrus betulaefolia) phloem

Li, Hao; Zhang, Yi; Wang, Shengnan; Zhang, Wenna; Xu, Chaoran; Yu, Yunfei; Li, Tianzhong; Jiang, Feng; Wei, Li

doi:10.1016/j.plantsci.2020.110419

Cited by 30 publications

(13 citation statements)

References 62 publications

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“…In this study, we established a VIGS technique to functionally characterize genes in pear floral buds. Previous investigations have confirmed that tobacco rattle virus (TRV) can infect apple and pear 42 – 44 . Therefore, TRV was used to silence DAM genes in dormant pear buds to further clarify DAM functions during bud dormancy transition.…”

Section: Discussionmentioning

confidence: 95%

High-quality genome assembly of 'Cuiguan' pear (Pyrus pyrifolia) as a reference genome for identifying regulatory genes and epigenetic modifications responsible for bud dormancy

et al. 2021

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Dormancy-associated MADS-box (DAM) genes serve as crucial regulators of the endodormancy cycle in rosaceous plants. Although pear DAM genes have been identified previously, the lack of a high-quality reference genome and techniques to study gene function have prevented accurate genome-wide analysis and functional verification of such genes. Additionally, the contribution of other genes to the regulation of endodormancy release remains poorly understood. In this study, a high-quality genome assembly for 'Cuiguan' pear (Pyrus pyrifolia), which is a leading cultivar with a low chilling requirement cultivated in China, was constructed using PacBio and Hi-C technologies. Using this genome sequence, we revealed that pear DAM genes were tandemly clustered on Chr8 and Chr15 and were differentially expressed in the buds between 'Cuiguan' and the high-chilling-requirement cultivar 'Suli' during the dormancy cycle. Using a virus-induced gene silencing system, we determined the repressive effects of DAM genes on bud break. Several novel genes potentially involved in the regulation of endodormancy release were identified by RNA sequencing and H3K4me3 chromatin immunoprecipitation sequencing analyses of 'Suli' buds during artificial chilling using the new reference genome. Our findings enrich the knowledge of the regulatory mechanism underlying endodormancy release and chilling requirements and provide a foundation for the practical regulation of dormancy release in fruit trees as an adaptation to climate change.

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Section: Discussionmentioning

confidence: 95%

High-quality genome assembly of 'Cuiguan' pear (Pyrus pyrifolia) as a reference genome for identifying regulatory genes and epigenetic modifications responsible for bud dormancy

et al. 2021

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“…More thorough research found that the participation of systemic mobile mRNAs such as CmNACP , SHR , KNOTTED1 , StBEL5 , PFP-LET6 , and CmGAI in the development of roots, apical meristem, leaves, and underground tubers in various plant species involves their long-distance transport between organs, facilitated by companion cells and sieve elements of the phloem system, and constitutes an important aspect of non-cell-autonomous signaling in plants [ 33 – 37 ]; likewise, the movement of CmWRKYP , CmPP2 , Cmlec17 , CmPP16 , and SlSSI mRNAs is involved in stress responses [ 38 – 42 ]. With bioinformatic tools, numerous mRNAs and non-coding RNAs have been shown to move from source into sink organs in response to chilling, phosphorus deficiency, and salt stress resistance by using grafting [ 23 , 43 – 45 ]. Gene Ontology (GO) analyses have revealed a broad range of biological processes and molecular functions for mobile mRNAs [ 44 , 46 , 47 ]; however, little is known about how such mobile mRNAs, as rootstock–scion interactional signals, are involved in improving chilling tolerance in grafted cucumber.…”

Section: Introductionmentioning

confidence: 99%

Rootstock–scion exchanging mRNAs participate in the pathways of amino acid and fatty acid metabolism in cucumber under early chilling stress

Liu

Wang

Zhang

et al. 2022

Horticulture Research

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Cucumber (Cucumis sativus L.) often experiences chilling stress that limits their growth and productivity. Grafting is widely used to improve abiotic stress resistance by alternating a vigorous root system, suggesting there exists systemic signals communication between distant organs. mRNAs are reported to be evolving in fortification strategies by long-distance signaling when plants suffering from chilling stress. However, the potential function of mobile mRNAs alleviating chilling stress in grafted cucumber is still unknown. Here, the physiological changes, mobile mRNAs profiling, transcriptomic and metabolomic changes in above- and underground tissues of all graft combinations of cucumber and pumpkin responding to chilling stress were established and analyzed comprehensively. The co-relationship between the cluster of chilling-induced pumpkin mobile mRNAs with Differentially Expressed Genes (DEGs) and Differentially Intensive Metabolites (DIMs) revealed that four key chilling-induced pumpkin mobile mRNAs were highly related to glycine, serine and threonine synthesis and fatty acid β-oxidative degradation metabolism in cucumber tissues of heterografts. The verification of mobile mRNAs, potential transport of metabolites and exogenous application of key metabolites of glycerophospholipid metabolism pathway in cucumber seedlings confirmed that the role of mobile mRNAs in regulating chilling responses in grafted cucumber. Our results build a link between the long-distance mRNAs of chilling-tolerant pumpkin and the fatty acid β-oxidative degradation metabolism of chilling-sensitive cucumber. It helps to uncover the mechanism of signaling interaction between scion and stock responding to chilling tolerant in grafted cucumber.

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“…Finally, the gene function can be assessed based on the plant phenotype and physiological index alteration [9]. VIGS can be developed rapidly and has been applied to functional gene identi cation in eshy fruits, such as tomato [8,10], strawberry [11][12][13], apple [14][15], grape [16], citrus [17], loquat [18], pear [19][20], peach [21][22] , and sweet cherry [23][24]. However, there are no reports on VIGS in banana.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a virus-induced gene silencing system and functional elucidation of MaBAM9b in postharvest banana fruits

liu

Zhang

et al. 2020

Preprint

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Abstract Background: The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be improved. The banana is a typical starch conversion fruit, and β-amylase is a key enzyme that may play an important role in starch degradation during the ripening process. Musa acuminata β-amylase 9b (MaBAM9b) is closely related to starch degradation. However, its exact function in starch degradation has not been demonstrated in banana. Stable genetic transformation to identify gene function is time- and energy-consuming. Thus, an efficient and rapid method is needed for functional identification. Virus-induced gene silencing (VIGS) is a reverse-genetics method based on RNA-mediated antiviral plant defense that has been used to rapidly identify gene functions in plants. The aim of this study is to optimize a VIGS system and functional elucidation of MaBAM9b in postharvest banana fruits. Results: Using 0.5% iodine-potassium-iodide (I2-KI) staining for 150 s, we found that 1:3 TRV1:TRV2-MaBAM9b cultivated at 30 mmHg for 30 s to an optical density (OD) of 0.8 at 600 nm, and kept on Murashige & Skoog (MS) media for 5 days produced the best silencing results. Under these conditions, the total starch content was greatly increased, whereas the β-amylase activity, soluble sugar content, and expression of endogenous MaBAM9b greatly decreased. Conclusions: The system described here is particularly useful for studying genes and networks involved in starch conversion in fruit, which alone would not produce a visual phenotype. This system will provide a platform for functional genomics and fruit quality improvement in the banana.

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A constitutive and drought-responsive mRNA undergoes long-distance transport in pear (Pyrus betulaefolia) phloem

Cited by 30 publications

References 62 publications

High-quality genome assembly of 'Cuiguan' pear (Pyrus pyrifolia) as a reference genome for identifying regulatory genes and epigenetic modifications responsible for bud dormancy

High-quality genome assembly of 'Cuiguan' pear (Pyrus pyrifolia) as a reference genome for identifying regulatory genes and epigenetic modifications responsible for bud dormancy

Rootstock–scion exchanging mRNAs participate in the pathways of amino acid and fatty acid metabolism in cucumber under early chilling stress

Optimization of a virus-induced gene silencing system and functional elucidation of MaBAM9b in postharvest banana fruits

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