A Constraint-based modeling approach to reach an improved chemically defined minimal medium for recombinant antiEpEX-scFv production by Escherichia coli

Escherichia coli is an attractive and cost-effective cell factory for producing recombinant proteins such as single-chain variable fragments (scFvs). AntiEpEX-scFv is a small antibody fragment that has received considerable attention for its ability to target the epithelial cell adhesion molecule (EpCAM), a cancerassociated biomarker of solid tumors. Due to its metabolic burden, scFv recombinant expression causes a remarkable decrease in the maximum speci c growth rate of the scFv-producing strain. In the present study, a genome-scale metabolic model (GEM)-guided engineering strategy is proposed to identify gene targets for improved antiEpEX-scFv production in E. coli. For improving scFv production, ux balance analysis (FBA) and FVSEOF methods identi ed a number of potential genetic engineering targets localized in the glucose uptake system and pentose phosphate pathway. From the predicted targets, glk gene encoding glucokinase was chosen to be overexpressed in the parent strain Escherichia coli BW25113 (DE3). By overexpressing glk, the growth capacity of the recombinant E. coli strain was recovered. Moreover, the engineered strain with glk overexpression successfully lead to increased scFv production. This approach can be considered for the improvement of other recombinant proteins production.

show abstract

Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production

Behravan

Hashemi

Marashi

et al. 2022

Biotechnol Lett

Self Cite

View full text Add to dashboard Cite

show abstract

Identification and analysis of the key genes for Escherichia coli heterologous protein expression by transcriptomic profiling

Chen,

Dong,

Jiang

et al. 2024

Mol Biol Rep

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A Constraint-based modeling approach to reach an improved chemically defined minimal medium for recombinant antiEpEX-scFv production by Escherichia coli

Cited by 2 publications

References 45 publications

Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production

Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production

Identification and analysis of the key genes for Escherichia coli heterologous protein expression by transcriptomic profiling

Contact Info

Product

Resources

About