This research focuses on Wayanad District within the Nilgiri Biosphere Reserve of the Western Ghats, a renowned hotspot forlichen diversity. A thorough investigation documented 10 distinct Parmotrema species, with one newly identified species (Parmotremaclavuliferum). Each species was comprehensively described, encompassing their morphological, chemical, and biogeographicalcharacteristics. The core objective of this study revolves around conservation and sustainable utilization of this valuable bioresource.This research contributes to our understanding of lichen ecosystems, particularly in regions facing diverse threats, and underscores the importance of the Wayanad District within the broader context of biodiversity conservation.