A contribution to the maintenance of phytopathogenic virus strains on tissue culture plantlets

Schenk, G.; Neitzel, K.

doi:10.1007/bf02357534

Cited by 8 publications

(3 citation statements)

References 4 publications

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“…Virus yield from in vitro node cultures of N. benthamiana was 2 mg/ 100g. Schenk & Neitzel [19] reported comparable concentrations of PVX or PVY in tissue culture plantlets and in glasshouse-grown plants, based on ELISA results. The purification of a greater amount of GVA from in vitro cultures than from leaves of greenhouse-maintained N. benthamiana might reflect a higher concentration of GVA in the cultures than in the leaves, but this has not been investigated.…”

Section: Resultsmentioning

confidence: 99%

“…Numerous viruses have been detected in in vitro cultures by enzyme-linked immunosorbent assay (ELISA), immunosorbent electron microscopy (ISEM) or bioassays in susceptible herbaceous indicator plants [3,4,13,14,16,17,20] and it has been suggested that such cultures could be used for the maintenance of viruses and strains [15,19]. To the best of our knowledge, only one report has been published to date advocating the use of in vitro cultures for the purification of plant viruses [9].…”

Section: Introductionmentioning

confidence: 99%

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Use of in vitro cultures of Nicotiana benthamiana for the purification of grapevine virus A

Monette¹,

James²

1990

Plant Cell Tiss Organ Cult

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Seedlings of Nicotiana benthamiana were inoculated with grapevine virus A (GVA). Three weeks later, upon systemic symptom expression, cultures were established in vitro using single nodes from these plants. GVA was purified from these cultures and from leaves of GVA-infected N. benthamiana plants maintained in the greenhouse. More virus was obtained from the proliferating in vitro node cultures than from the leaves. The use of in vitro cultures for virus purification represents a valuable practical application of plant tissue culture techniques.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of in vitro cultures of Nicotiana benthamiana for the purification of grapevine virus A

Monette¹,

James²

1990

Plant Cell Tiss Organ Cult

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“…Typically, PVX-based vectors are used for the infection of greenhouse-grown plants. However, it was reported that plant viruses, including PVX, can persist in tissue cultures for years [ 34 , 35 ], thereby suggesting the possibility of using plant viral vectors for transient expression of recombinant proteins in plant tissue cultures.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro

Sindarovska

Кучук

2021

Plants

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Plant molecular farming has a great potential to produce valuable proteins. Transient expression technology provides high yields of recombinant proteins in greenhouse-grown plants, but every plant must be artificially agroinfiltrated, and open greenhouse systems are less controlled. Here, we propose to propagate agrobacteria-free plants with high-efficient long-term self-replicated transient gene expression in a well-controlled closed in vitro system. Nicotiana benthamiana plant tissue culture in vitro, with transient expression of recombinant GFP, was obtained through shoot induction from leaf explants infected by a PVX-based vector. The transient expression occurs in new tissues and regenerants due to the natural systemic distribution of viral RNA carrying the target gene. Gene silencing was delayed in plants grown in vitro, and GFP was detected in plants for five to six months. Agrobacteria-free, GFP-expressing plants can be micropropagated in vitro (avoiding an agroinfiltration step), “rejuvenated” through regeneration (maintaining culture for years), or transferred in soil. The mean GFP in the regenerants was 18% of the total soluble proteins (TSP) (0.52 mg/g of fresh leaf weight (FW). The highest value reached 47% TSP (2 mg/g FW). This study proposes a new method for recombinant protein production combining the advantages of transient expression technology and closed cultural systems.

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Breeding Potatoes for Long‐Day, Temperate Climates

Tarn¹,

Tai²,

Long³

et al. 1992

Plant Breeding Reviews

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A contribution to the maintenance of phytopathogenic virus strains on tissue culture plantlets

Cited by 8 publications

References 4 publications

Use of in vitro cultures of Nicotiana benthamiana for the purification of grapevine virus A

Use of in vitro cultures of Nicotiana benthamiana for the purification of grapevine virus A

Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro

Breeding Potatoes for Long‐Day, Temperate Climates

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